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Showing: 4601 - 4620 of 66047 results

  5. mCherry-Ezrin-C-14

    Plasmid
    #55042
    Purpose
    Localization: Focal Adhesions/Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NM_003379.4 (EZR Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. pIFU002

    Plasmid
    #174299
    Purpose
    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the N501Y mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination
    Depositor
    Timothy Whitehead
    Publication
    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:
    Insert
    Spike RBD N343Q, N501Y (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HA and cMyc
    Expression
    Yeast
    Mutation
    N343Q, N501Y
    Available since
    Sept. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    174299-plasmid-map-sequence-id-342196

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  7. pIFU001

    Plasmid
    #174298
    Purpose
    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the E484K mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination
    Depositor
    Timothy Whitehead
    Publication
    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:
    Insert
    Spike RBD N343Q, E484K (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HA and cMyc
    Expression
    Yeast
    Mutation
    N343Q, E484K
    Available since
    Sept. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    174298-plasmid-map-sequence-id-342193

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  8. pTub-alpha1-EB3-NeonGreen-T2A-iLifeact-mCherry

    Plasmid
    #175261
    Purpose
    labelling of microtubule plus end and F-actin structures
    Depositor
    Frank Bradke, Sebastián Dupraz
    Publication
    Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EB3-NeonGreen-T2A-iLifeact-mCherry (MAPRE3 Human)
    Tags
    mCherry (on iLifeact) and mNeonGreen (on EB3)
    Expression
    Mammalian
    Mutation
    None (wt)
    Promoter
    pTub-alpha1
    Available since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    175261-plasmid-map-sequence-id-349034

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  9. p426_Cas9_gRNA-ARS1622b

    Plasmid
    #87403
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1622b sequence GTCACGTTCCTGAGGTTACT in yeast chromosome 16.
    Depositor
    Aindrila Mukhopadhyay
    Publication
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1622b
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87403-plasmid-map-sequence-id-193446

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  10. p426_Cas9_gRNA-YPRCd15c

    Plasmid
    #87404
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting YPRCd15c sequence AATCCGAACAACAGAGCATA in yeast chromosome 14.
    Depositor
    Aindrila Mukhopadhyay
    Publication
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting YPRCd15c
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87404-plasmid-map-sequence-id-193447

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  11. p426_Cas9_gRNA-ARS106a

    Plasmid
    #87382
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS106a sequence ATACGGTCAGGGTAGCGCCC in yeast chromosome 1.
    Depositor
    Aindrila Mukhopadhyay
    Publication
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS106a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87382-plasmid-map-sequence-id-193424

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  12. p426_Cas9_gRNA-ARS308a

    Plasmid
    #87384
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS308a sequence CACTTGTCAAACAGAATATA in yeast chromosome 3
    Depositor
    Aindrila Mukhopadhyay
    Publication
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS308a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87384-plasmid-map-sequence-id-193426

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  15. Dendra2-ER-5

    Plasmid
    #57716
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 490 / 553, Emission: 507 / 573
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ER (CALR Human)
    Tags
    Dendra2
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available since
    Jan. 6, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  16. EGFP-ER-14

    Plasmid
    #56432
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Publication
    Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ER (CALR Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available since
    Dec. 23, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. mEmerald-Cofilin-C-10

    Plasmid
    #54047
    Purpose
    Localization: Actin, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NM_005507.2 (CFL1 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. HSPA5A

    Plasmid
    #42447
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSPA5A (HSPA5 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. tdTomato-Calreticulin-N-16

    Plasmid
    #58074
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Calreticulin (CALR Human)
    Tags
    tdTomato
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 4601 - 4620 of 66047 results