We narrowed to 7,670 results for: 11
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Plasmid#209906PurposeTo monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only
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STARR-seq luciferase validation vector_ORI_SV40
Plasmid#99309PurposeLuciferase validation vector with SV40 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorInsertSV40 enhancer
UseLuciferase; Starr-seq luciferase validation vectorExpressionMammalianAvailable SinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-U6-UL29-egfp-U3-UL8
Plasmid#166694PurposeTo express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (EGFP version).DepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 p37deltaSEP
Plasmid#113501PurposeBacterial expression of GST-tagged p37 lacking the SEP domainDepositorInsertp37 (Ubxn2b Mouse)
TagsGST-PreScission protease siteExpressionBacterialMutationdelta 140-206Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Cx26
Plasmid#69021PurposeExpresses rat Cx26 (Gjb2 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.DepositorAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AvicFP3
Plasmid#129506PurposeExpresses AvicFP3 constitutively in E. coli (most strains)DepositorInsertAvicFP3
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promote…Available SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Puro-sgNUAK2
Plasmid#138693PurposeExpresses a human NUAK2-targeting sgRNA and Cas9DepositorAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe-GW-HA-RINT1
Plasmid#39841DepositorAvailable SinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pN1-RnKIF5C(1-560)-Halo-Flag
Plasmid#188071PurposeMammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tagsDepositorAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFR D837A
Plasmid#11014PurposeRetroviral construct for expressing human EGFR with D837A mutation in human cellsDepositorAvailable SinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pHR-Hsp-Fluc-PGK-Rluc-mCherry
Plasmid#191863PurposeHeat inducible Fluc reporter with constitutive RlucDepositorInsertsFirefly luciferase Fluc
Renilla luciferase Rluc
UseLentiviralTagsP2A-mCherryExpressionMammalianPromoterHSPA7 promoter and PGK promoterAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-S81A
Plasmid#171241PurposeMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser81Ala (AR-S81A)DepositorInsert3xFlag-tagged human androgen receptor Ser81Ala mutant (AR Human)
TagsFlagExpressionMammalianMutationAR-Ser81Ala (AR-S81A)PromoterCMVAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-KRAS
Plasmid#13919DepositorAvailable SinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
CDK4-3XFlag-pBabe
Plasmid#73558Purposevector encoding a fusion protein between CDK4 and 3 Flag tagsDepositorAvailable SinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQHA-USP7 CS puroR
Plasmid#46754PurposeRetroviral vector that expresses catalytically inactive form of HA-tagged human USP7DepositorInsertUSP7 (USP7 Human)
UseRetroviralTagsHAExpressionMammalianMutationC223S--catalytically inactivePromoterCMVAvailable SinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCas9_sgRNA_0
Plasmid#70763Purposeexpresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicatingDepositorInsertsU6 promoter
cas9
tnos terminator
UseSelf-replicating in ustilago maydis, conferring c…TagsN-terminal NLS, C-terminal HA-tag +NLSMutationStreptococcus pyogenes gene codon-optimized for U…PromoterU6 from Ustilago maydis and synthetic otef promot…Available SinceNov. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2-MT mouse Axin (Axin MTFu1)
Plasmid#21287DepositorAvailable SinceDec. 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-Tlr4
Plasmid#27148DepositorInserttoll-like receptor 4 (Tlr4 Mouse)
Tags3x FLAGExpressionMammalianMutationNative signal peptide sequence (original amino ac…Available SinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-196, G175R)-Strep
Plasmid#196211PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G175R)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
TagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationG175RAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-196, G189E)-Strep
Plasmid#196212PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G189E)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
TagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationG189EAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only