We narrowed to 5,622 results for: crispr cas9 grna plasmid

1. pML107-VRG4

   Plasmid

   #232899

   Purpose

   Plasmid expressing Cas9 and gRNA TCGCATAGCCCAGTATACGT which targets the VRG4 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   VRG4 gRNA (VRG4 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   snR52

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pML104-HIS3-3

   Plasmid

   #232882

   Purpose

   Plasmid expressing Cas9 and gRNA AAACCTTTTTACTCCACGCA which targets the HIS3 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   HIS3 gRNA (HIS3 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   snR52

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pML104-HIS3-2

   Plasmid

   #232881

   Purpose

   Plasmid expressing Cas9 and gRNA CCAAGTTCGACAACTGCGTA which targets the HIS3 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   HIS3 gRNA (HIS3 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   snR52

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBUN411

   Plasmid

   #50581

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   3×FLAG-NLS-zCas9-NLS
   gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Tags

   3xFLAG and NLS

   Expression

   Mutation

   maize codon optimized

   Promoter

   OsU3p and Ubi1p

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHSN401

   Plasmid

   #50588

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   3×FLAG-NLS-zCas9-NLS
   gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Tags

   3xFLAG and NLS

   Expression

   Mutation

   maize codon optimized

   Promoter

   2×35Sp and AtU6-26p

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAS_gpyrG1

   Plasmid

   #90276

   Purpose

   (also pMST621-BB3_gpyrG1_cas9) CRISPR/Cas9 plasmid with gRNA for pyrG1, Cas9

   Depositor

   Michael Sauer

   Article

   Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

   Insert

   gRNA (pyrg1)
   

   Use

   A. niger

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAS_gpyrG2

   Plasmid

   #90277

   Purpose

   (also pMST620-BB3_gpyrG2_cas9) CRISPR/Cas9 plasmid with gRNA for site pyrG2, Cas9

   Depositor

   Michael Sauer

   Article

   Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

   Insert

   gRNA (pyrg2)
   

   Use

   A. niger

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDuRCC-K

   Plasmid

   #81193

   Purpose

   Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below., Please view…

   Promoter

   ROX3 and SNP52p

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDuRCC-N

   Plasmid

   #81194

   Purpose

   Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please view depositor comments below and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPR-cBEST-v2-SF14P

    Plasmid

    #209448

    Purpose

    improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the SF14P promoter

    Depositor

    Tilmann Weber

    Article

    Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

    Insert

    Codon optimized APOBEC1-nCas9-UGI fusion protein
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PtipA; sgRNA expression from SF14P promoter

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPR-cBEST-v2-kasOP*

    Plasmid

    #209447

    Purpose

    improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoter

    Depositor

    Tilmann Weber

    Article

    Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

    Insert

    Codon optimized APOBEC1-nCas9-UGI fusion protein
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PtipA; sgRNA expression from kasOP* promoter

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp

    Plasmid

    #99696

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAV

    Depositor

    George Church

    Insert

    dCas9 and gRNA targeting Afp (Afp Synthetic)
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSc1-puro

    Plasmid

    #80438

    Purpose

    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell, and also confers resistance to puromycin

    Depositor

    Ervin Welker

    Article

    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

    Inserts

    sp Cas9 gRNA
    eGFP
    Puromycin resistance
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6, and hPGK

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CMV-dSa VP64 Actc1

    Plasmid

    #99691

    Purpose

    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Actc1 promoter, vector allows for activation of mouse Actc1, can be packaged and delivered as AAV

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG) (Actc1 Synthetic, Mouse)
    

    Use

    AAV and CRISPR

    Tags

    VP64

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CMV-dSa VP64 Neurog2

    Plasmid

    #99695

    Purpose

    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Neurog2 promoter, vector allows for activation of mouse Neurog2, can be packaged and delivered as AAV

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9 and gRNA targeting Neurog2 (gRNA: GGTATATAAGGGGTTTTAAG) (Neurog2 Synthetic, Mouse)
    

    Use

    AAV and CRISPR

    Tags

    VP64

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Sept. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDB4282

    Plasmid

    #98701

    Purpose

    A plasmid containing the 3' portion of the ura4 marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-ura4 system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    gRNA PCR template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDB4283

    Plasmid

    #98702

    Purpose

    A plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    gRNA PCR template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PX459-CTNNA1(α-catenin CRISPR KO)

    Plasmid

    #229708

    Purpose

    Knockout of a-catenin in mammalian cells using CRISPR-Cas9 technology

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pROS17

    Plasmid

    #107931

    Purpose

    TRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pROS10

    Plasmid

    #107924

    Purpose

    URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge