We narrowed to 2,467 results for: PUC19
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Plasmid#91654PurposeExpress sgRNA targeting human PTNDepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only
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pCBS-5738
Plasmid#249324PurposeTo express the EPEC DarT2(DLAA)-nScCas9(D10A) fusion protein in experiments for Append EditingDepositorInsertEPEC DarT2(DLAA)-nScCas9(D10A)
ExpressionYeastMutationDarT2(G49D, M86L, R92A, R193A) and nScCas9(D10A)Available SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCBS-5753
Plasmid#249325PurposeTo express the EPEC dDarT2(DLAA)-nScCas9(D10A) fusion protein in experiments for Append EditingDepositorInsertEPEC dDarT2(DLAA)-nScCas9(D10A)
ExpressionYeastMutationDarT2(G49D, M86L, R92A, E,170A, R193A) and nScCas…Available SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pMT1375
Plasmid#228314PurposeConstitutive expression of mutant rTetTADepositorInsertrtetTA mutant
UseSynthetic BiologyTagsThree copies of VP16 (VP48)ExpressionYeastMutationK8NPromoterKpGAPDH promoterAvailable SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-MAP4
Plasmid#227296PurposeDonor template for mStayGold insertion into the N-terminus of the MAP4 locus. For microtubule visualization. To be co-transfected with sgRNAplasmid px330-PITCh-MAP4 (Addgene #227295)DepositorInsertMAP4 Homology Arms flanking a mStayGold Tag (MAP4 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-CEP192
Plasmid#227289PurposeDonor template for mStayGold insertion into the C-terminus of the CEP192 locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PITCh-CEP192 (Addgene #227288)DepositorInsertCEP192 Homology Arms flanking a mStayGold Tag (CEP192 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBV90
Plasmid#204601PurposeExpresses OsTir1-3X Myc from S. cerevisiae ADH1 promoterDepositorAvailable SinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJQR3
Plasmid#218289PurposeIntroduction of a Zif268-hER-VP16 expression cassette and a LmrA-Mig1C expression cassette, use together with pToxEXPR6DepositorInsertHO(-253,-1)-pADH1>LmrA>MIG1(aa123-504)>tMIG1-tYGL036WExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pToxAmp12
Plasmid#218286PurposeMulti-copy integration of heterologous genes (AeBlue and RelB) through co-transformation with ToxAmp (toxin-antitoxin-driven gene amplification) modulesDepositorInsertHO(-253, -1)-pCRT1>RelB>tPDC1-pTDH3>AeBlue>tsynth7-ARS712-HO(-731, -264)
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pToxAmp1
Plasmid#218285PurposeAmplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanismDepositorInsertHO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only