We narrowed to 12,292 results for: BASE

1. pDisplay-GRAB-gDA3h-IRES-mCherry-CAAX

   Plasmid

   #208693

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3h and a membrane-localized mcherry in mammalian cells

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_gDA3h
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pW5Y_Apr

   Plasmid

   #158211

   Purpose

   BAC-based, lacUV5 regulon and Cre recombinase in loxP and lox5171 sites

   Depositor

   Yasuo Yoshikuni

   Article

   Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. A3Bi-ctd-Cas9n-UGI-NLS

   Plasmid

   #109426

   Purpose

   Expresses the C-terminal catalytic domain of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Insertion of an L1 intron into the coding sequenc…

   Promoter

   CMV

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDisplay-GRAB-rDA3h-IRES-EGFP-CAAX

   Plasmid

   #208697

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h and a membrane-localized EGFP in mammalian cells

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA3h
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-GFAP-eGFP-shLacZ

   Plasmid

   #223222

   Purpose

   mir30 based shRNA strategy, control shRNA

   Depositor

   Li-Huei Tsai

   Article

   Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

   Insert

   shLacZ
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDisplay-GRAB-rDA2h-IRES-EGFP-CAAX

   Plasmid

   #208695

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2h and a membrane-localized EGFP in mammalian cells

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA2h
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-UBC-S2-cAMPr

   Plasmid

   #160723

   Purpose

   Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter cAMPr (cAMP indicator), HA tag, and S2 protein scaffold.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   S2-cAMPr
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human ubiquitin C (UBC) promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSBbi-moxGFP-P2A-T2A-Puro

   Plasmid

   #176893

   Purpose

   Sleeping Beauty transposon-based vector for mammalian polycistronic expression of moxGFP and two additional genes of interest

   Depositor

   Jorge Luis Arias-Arias

   Article

   Acon et al iScience. 2021 Nov 8;24(12):103407. doi:

   Insert

   moxGFP-P2A-T2A
   

   Use

   Transposon

   Tags

   moxGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKK-BI16-ORF1-3C-mRuby3_ORF2-TEV-mClover3

   Plasmid

   #105802

   Purpose

   Concomitant expression of two proteins. One protein expressed with mRuby3, cleavable by 3C; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   ORF1: 3C-mRuby3; ORF2: TEV-mClover3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-UBC-S3-ExRaiAKAR

    Plasmid

    #160724

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-AKAR (PKA indicator), V5 tag, and S3 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S3-ExRaiAKAR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-UBC-S4-ExRaiCKAR

    Plasmid

    #160725

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-CKAR (PKC indicator), OLLAS tag, and S4 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S4-ExRaiCKAR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCJ207

    Plasmid

    #162680

    Purpose

    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1(+)-FluoSTEP-ICUE

    Plasmid

    #181845

    Purpose

    Genetically encoded FRET-based sensor for monitoring cAMP dynamics near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    FluoSTEP-ICUE (RAPGEF3 Human)
    

    Use

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA

    Plasmid

    #136652

    Purpose

    Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-UBC-S2-RAB_EKARev

    Plasmid

    #160726

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S2 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S2-RAB_EKARev
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKK-BI16-FLAG-3C-ORF1_mClover3-TEV-ORF2

    Plasmid

    #105801

    Purpose

    Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C or enterokinase; second protein expressed with mClover3, cleavable by TEV protease; tags positions: N-termini.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: FLAG-3C; ORF2: mClover3-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. A3Ai E72A-Cas9n-UGI-NLS

    Plasmid

    #109430

    Purpose

    Expresses catalytically inactive human APOBEC3A with an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A E72A Catalytic Mutant (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Insertion of an L1 intron into the coding sequenc…

    Promoter

    CMV

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-UBC-S3-RAB_EKARev

    Plasmid

    #160727

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S3 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S3-RAB_EKARev
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Venus

    Plasmid

    #105805

    Purpose

    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Venus, cleavable by TEV. Useful for protein interaction analysis by FRET.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: 3C-Cerulean; ORF2: TEV-Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAav-TP53-T2A-BirA*

    Plasmid

    #115652

    Purpose

    rAAV-based donor template for genome engineering of the TP53 protein C-terminus containing a T2A-BirA* module and a selection cassette

    Depositor

    Sven Eyckerman

    Article

    Vandemoortele et al J Proteome Res. 2018 Dec 10. doi: 10.102

    Inserts

    TP53 (TP53 Human)
    TP53 (TP53 Human)
    T2A
    BirA*
    

    Use

    AAV

    Tags

    BirA* and T2A

    Expression

    Mutation

    Homology region 1 and Homology region 2

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-UBC-S1-jRCaMP1a

    Plasmid

    #160728

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter jRCaMP1a (calcium indicator), Xpress tag, and S1 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S1-jRCaMP1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGAPTrap-IRESMuro

    Plasmid

    #82336

    Purpose

    Base vector targeting genes to the GAPDH locus of human cells. Inserted genes are expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    IRESMuro
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAav-MDM2-PQS2-3xHA

    Plasmid

    #84886

    Purpose

    rAAV-based template for genome engineering of the MDM2 protein C-terminus containing PQS2 and 3xHA tags and a selection cassette

    Depositor

    Sven Eyckerman

    Article

    Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

    Inserts

    MDM2 genomic sequence HR1 (MDM2 Human)
    MDM2 genomic insert HR2 (MDM2 Human)
    

    Use

    AAV

    Tags

    PQS2 3xHA

    Expression

    Mutation

    Promoter

    no

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCJ199

    Plasmid

    #162672

    Purpose

    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with C-terminal His tag, codon optimized for expression in E. coli K12.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    Putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with signal peptide
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKK-BI16-ORF1-3C-FLAG_ORF2-TEV-mClover3

    Plasmid

    #105803

    Purpose

    Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C protease; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: 3C-FLAG; ORF2: TEV-mClover3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA

    Plasmid

    #136659

    Purpose

    Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD9:TH1477 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCJ203

    Plasmid

    #162678

    Purpose

    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    Putative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deltaF2-A75; codon optimized for expression in E.…

    Promoter

    T7/lac

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber

    Plasmid

    #105806

    Purpose

    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: 3C-Cerulean; ORF2: TEV-Amber

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3-Lyso-InPTapp

    Plasmid

    #236000

    Purpose

    Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4)P2. Targeted to lysosome surface.

    Depositor

    Jin Zhang

    Article

    Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

    Insert

    Lyso-InPTapp (PLEKHA1, LAMP1 Synthetic, Human)
    

    Use

    Tags

    Citrine, Full-length LAMP1, and truncECFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PB-2G-CNCB_sgRBBP6-68

    Plasmid

    #237555

    Purpose

    A piggybac-based vector containing mouse U6 promoter-driven RBBP6 sgRNA #6, human U6 promoter-driven RBBP6 sgRNA #8 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

    Depositor

    Kazutoshi Takahashi

    Article

    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

    Insert

    RBBP6 (RBBP6 Human)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6 promoter and mouse U6 promoter, human U6…

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLentiSIV hSyn HA-NLS-SpCas9-NLS WPRE

    Plasmid

    #236242

    Purpose

    Lentiviral expression of SpCas9

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    SpCas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCS2-MYC(Δ1-143)-GFP-IRES-mCherry (ΔTAD)

    Plasmid

    #231233

    Purpose

    MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) for transient expression in mammalian cells

    Depositor

    Michael Rape

    Article

    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TAD deleted (delta1-143); numbering based on sequ…

    Promoter

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1+FLAG-KrascomQ61R

    Plasmid

    #206845

    Purpose

    To express mouse Kras encoded by common mouse codons (to increase expression) and with a Q61R mutation with an N-terminal FLAG tag.

    Depositor

    Christopher Counter

    Article

    Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

    Insert

    Kras (Kras Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Codons altered to most optimum based on mouse cod…

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV2-UbC-3xNLS-mScarlet-I

    Plasmid

    #191099

    Purpose

    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

    Depositor

    Pantelis Tsoulfas, Murray Blackmore

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    3xNLS-mScarlet-I
    

    Use

    AAV and Synthetic Biology

    Tags

    Three repeats of the nuclear localizzation seque…

    Expression

    Mammalian

    Mutation

    optimized to human codon usage

    Promoter

    UbC

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. K-to-R mutant NPRL3(1-569) in pLJM60, codon-optimized

    Plasmid

    #184563

    Purpose

    CMV-driven expression of an untagged mutant NPRL3 (core subunit of GATOR1) — codon-optimized sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.

    Depositor

    Kacper Rogala

    Article

    Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

    Insert

    C16orf35, CGTHBA, FFEVF3, HS-40, MARE, NPR3, RMD11 (NPRL3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    All lysines were mutated to arginines. Codon-opti…

    Promoter

    CMV

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMG18311_2xUGI_3xHA

    Plasmid

    #136657

    Purpose

    Expresses St1BE4max-LMG18311 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD9:LMG18311 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_CNRZ1066_2xUGI_3xHA

    Plasmid

    #136654

    Purpose

    Expresses St1BE4max-CNRZ1066 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD9:CNRZ1066 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSBbi-Pur-Lyn-AktAR2

    Plasmid

    #125197

    Purpose

    SB-transposon plasmid for stable expression of lipid raft-targeted Lyn-AktAR2 variant of FRET-based AKT activity reporter

    Depositor

    Eric Davis

    Article

    Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

    Insert

    Lyn-AktAR2
    

    Use

    Transposon

    Tags

    Lyn tag (GCIKSKRKD), mCerulean3 and cpVenus[E172]

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. EF1a-EGFP (no signal control)

    Plasmid

    #170167

    Purpose

    Mammalian Expression of no signal control

    Depositor

    Christopher Moxham

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. EF1a-EGFP-SKL

    Plasmid

    #170166

    Purpose

    Mammalian Expression of Ef1a-EGFP-SKL

    Depositor

    Christopher Moxham

    Insert

    EGFP-SKL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SKL

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. EF1a-EGFP-RSKL

    Plasmid

    #170165

    Purpose

    Mammalian Expression of Ef1a-EGFP-RSKL

    Depositor

    Christopher Moxham

    Insert

    EGFP-RSKL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    RSKL

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. EF1a-EGFP-KANL

    Plasmid

    #170164

    Purpose

    Mammalian Expression of Ef1a-EGFP-KANL

    Depositor

    Christopher Moxham

    Insert

    EGFP-KANL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    KANL

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. EF1a-ABCD3-RFP-Puro

    Plasmid

    #170168

    Purpose

    Mammalian Expression of Ef1a-ABCD3-RFP-Puro

    Depositor

    Christopher Moxham

    Insert

    ABCD3-RFP-Puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. MS2392.EF1a.SLC6A1.WT.P2A.BLAST.JL.p159

    Plasmid

    #170170

    Purpose

    Lentiviral Expression of EF1a.SLC6A1.WT

    Depositor

    Christopher Moxham

    Insert

    SLC6A1 (SLC6A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    n/a

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. MS2392.EF1a.SLC6A1.WT.EGFP.P2A.BLAST.JL.p164

    Plasmid

    #170169

    Purpose

    Lentiviral Expression of EF1a.SLC6A1.WT.EGFP

    Depositor

    Christopher Moxham

    Insert

    SLC6A1 (SLC6A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    n/a

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. MS2592.EF1a.SLC6A1.S295L.RFP.P2A.PURO.JL.p163

    Plasmid

    #170174

    Purpose

    Lentiviral Expression of EF1a.SLC6A1.S295L.RFP

    Depositor

    Christopher Moxham

    Insert

    SLC6A1 (SLC6A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S295L

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. MS2592.EF1a.SLC6A1.A288V.P2A.PURO.JL.p166

    Plasmid

    #170171

    Purpose

    Lentiviral Expression of EF1a.SLC6A1.A288V

    Depositor

    Christopher Moxham

    Insert

    SLC6A1 (SLC6A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    A288V

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pInt_attP1_kanR

    Plasmid

    #205294

    Purpose

    Integrating plasmid for ORBIT in E. coli. Standard attP site (#1).

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pInt_attP2_kanR

    Plasmid

    #205297

    Purpose

    Integrating plasmid for ORBIT in E. coli. Orthogonal attP site (#2).

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pInt_attP3_chlorR

    Plasmid

    #205298

    Purpose

    Integrating plasmid for ORBIT in E. coli. Orthogonal attP site (#3).

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge