We narrowed to 8,140 results for: sars

1. pCMV-myc-mIFITM3

   Plasmid

   #58458

   Purpose

   Expresses murine IFITM3 with an N-terminal myc tag in mammalian cells.

   Depositor

   Jacob Yount

   Article

   Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

   Insert

   IFITM3 (Ifitm3 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available since

   Sept. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pHcPro (GB0067)

   Plasmid

   #68196

   Purpose

   Provides the CDS of the Tobacco echt virus HcPro protein as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   TEV HcPro coding region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-FLAG-eSpCas9

   Plasmid

   #126769

   Purpose

   Expression of increased fidelity eSpCas9 in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A

   Promoter

   T7

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTAtUbq3 (GB0219)

   Plasmid

   #68192

   Purpose

   Provides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtUbq3 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG415Gal_Gcn4 SATtoG KtoRHA

   Plasmid

   #232994

   Purpose

   Galactose iduced expression of Gcn4 SATtoG KtoRHAin yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 SATtoG KtoR
   

   Use

   Tags

   3xHA and TEV cleavage site

   Expression

   Yeast

   Mutation

   S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG415Gal_Gcn4 SATtoG KtoR

   Plasmid

   #232961

   Purpose

   Galactose iduced expression of Gcn4 ATtoG KtoR in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 SATtoG KtoR
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG415Gal_Gcn4 ILVtoED solvvol

   Plasmid

   #232958

   Purpose

   Galactose iduced expression of Gcn4 LVtoED solvvol in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 ILVtoED solvvol
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG415Gal_Gcn4 SATtoG KtoRGFP

   Plasmid

   #233006

   Purpose

   Galactose iduced expression of Gcn4 SATtoG KtoRGFP in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 SATtoG KtoR
   

   Use

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDest17_Gcn4 ILVtoED solvvol

   Plasmid

   #231858

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED solvvol

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 ILVtoED solvvol
   

   Use

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

   Promoter

   T7

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. GAL4(1-147)

    Plasmid

    #195029

    Purpose

    The control vector GAL4 (1-147) was generated by introducing a stop codon immediately after the GAL4 (1-147) sequence in the GAL4-Creb5 (1-128) vector

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    GAL4 (1-147)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. GL4.10 Prg4 promoter-luciferase

    Plasmid

    #195034

    Purpose

    Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    Prg4 promoter (PRG4 Bovine)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTC3cIfm

    Plasmid

    #193778

    Purpose

    Expresses frame-shifted cI under the control of PLTetO-1 promoter, p15A origin of replication, Chloramphenicol selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

    Insert

    Frame-shifted CI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLTetO-1

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. hCMV-IE1:RoLuc:bgH

    Plasmid

    #135290

    Purpose

    Transcriptional unit for constitutive expression of RoLuc in mammalian cells

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    CMV:RoLuc:bGH
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. hCMV-IE1:MetLuc:bgH

    Plasmid

    #135292

    Purpose

    Transcriptional unit for constitutive expression of MetLuc in mammalian cells

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    CMV:MetLuc:bGH
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-Flag-gp130dimer-stm2585

    Plasmid

    #174395

    Purpose

    Constitutively active gp130 dimer with codon-optimized stm2585 insert (replacing amino acids 140-179)

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    IL6ST cytosolic domain and sarA chimera
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. TB:3xDBE_RE:MiniP

    Plasmid

    #124529

    Purpose

    3 Copies of the Daf-16 family binding element

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    3 copies of the Daf-16 family binding element
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. myc-hIFITM3-P17A,P18A

    Plasmid

    #104364

    Purpose

    Expresses human IFITM3-P17A,P18A with an N-terminal myc tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Chesarino et al PLoS Pathog. 2015 Aug 11;11(8):e1005095.

    Insert

    IFITM3 (IFITM3 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    mutated amino acids as follows: P17A,P18A

    Promoter

    CMV

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDGB1alpha1_SF (GB0106)

    Plasmid

    #68232

    Purpose

    Twister plasmid to swap inserts from an alpha2 or alpha2R vector to any omega level vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Solanum lycopersicum intergenic region
    

    Use

    Synthetic Biology; Unspecified

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTAct2 (GB0210)

    Plasmid

    #68191

    Purpose

    Provides the Arabidopsis thaliana Actin2 gene terminator as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtAct2 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPMtb (GB0080)

    Plasmid

    #68167

    Purpose

    Provides the S. Lycopersicum metallothionein II-like protein (MTB) gene promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    SlMtb promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge