We narrowed to 9,363 results for: BLI

1. AAV-CAG-M-SPOTIT2(R279F)

   Plasmid

   #191493

   Purpose

   Green fluorescent-based opioid sensor for the mu opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter

   Depositor

   Wenjing Wang

   Insert

   M-SPOTIT2(R279F)
   

   Use

   AAV

   Mutation

   R279F in OR

   Promoter

   CAG

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-CAG-M-red-SPOTIT2(R279F)

   Plasmid

   #191495

   Purpose

   Red fluorescent-based opioid sensor for the mu opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter

   Depositor

   Wenjing Wang

   Insert

   M-red-SPOTIT2(R279F)
   

   Use

   AAV

   Mutation

   R279F in OR

   Promoter

   CAG

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PAS_HeSAHS4

   Plasmid

   #215648

   Purpose

   Expresses SUMO-tagged HeSAHS4 protein from tardigrade H. exemplaris in E. coli

   Depositor

   Pamela Silver

   Insert

   SAHS4
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PAS_RvSAHS4

   Plasmid

   #215646

   Purpose

   Expresses SUMO-tagged RvSAHS4 protein from tardigrade R. varieornatus in E. coli

   Depositor

   Pamela Silver

   Insert

   SAHS4
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX458-tRNA-SpCas9

   Plasmid

   #195183

   Purpose

   Vector for transient Cas9 and EGFP expression. Small tRNA promoter for sgRNA cloning by GoldenGate.

   Depositor

   Stephanie Roessler

   Insert

   Cas9
   

   Use

   CRISPR

   Promoter

   tRNA-Gln

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7CFE1_EVD68_VP12A_C107R

   Plasmid

   #212982

   Purpose

   Encodes EV-D68 MO/14/18947 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 VP12A C107R
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Mutation

   changed cysteine 107 from 2A to Arginine

   Promoter

   T7

   Available Since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7CFE1_EVD68_3ABC_C147R

   Plasmid

   #212978

   Purpose

   Encodes EV-D68 MO/14/18947 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 3ABC C147R
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Mutation

   changed Cysteine 147 in 3C to Arginine

   Promoter

   T7

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7CFE1_EVD68_3ABCD_C147R

   Plasmid

   #212980

   Purpose

   Encodes EV-D68 MO/14/18947 3ABCD with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 3ABCD C147R
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Mutation

   changed cysteine 147 from 3C to arginine

   Promoter

   T7

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7CFE1_EVD68_VP12A_N84T

   Plasmid

   #212983

   Purpose

   Encodes EV-D68 MO/14/18947 VP12A with telaprevir resistance mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 VP12A N84T
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Mutation

   changed Asparagine 84 from 2A to Threonine

   Promoter

   T7

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-Flag-RBM24-1~93

    Plasmid

    #208633

    Purpose

    for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa1~93

    Depositor

    Quanfu Ma

    Insert

    human RBM24 (ENST00000216146) aa 1~93 (RBM24 Human)
    

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    only aa 1~93, other residues not included

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-RPL3-Twstrep

    Plasmid

    #208623

    Purpose

    for E. coli expression of C-ter Twin Strep tagged human RPL3 (ENST00000216146)

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) (RPL3 Human)
    

    Tags

    6xHis and Twin Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAMW-Wnk

    Plasmid

    #203978

    Purpose

    Myc6 Wnk under actin promoter for cell culture

    Depositor

    Andreas Jenny

    Insert

    Wnk (Wnk Fly)
    

    Tags

    6xMyc

    Expression

    Insect

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. XBB.1.5+R403K

    Plasmid

    #208586

    Purpose

    Mammalian expression vector for expressing XBB.1.5+R403K SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation

    Depositor

    Ben Murrell

    Insert

    SARS-CoV-2 variant XBB.1.5+R403K spike (S )
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. XBB.1.5+F456L

    Plasmid

    #208585

    Purpose

    Mammalian expression vector for expressing XBB.1.5+F456L SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation

    Depositor

    Ben Murrell

    Insert

    SARS-CoV-2 variant XBB.1.5+F456L spike (S )
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. XBB.1.5+K478R

    Plasmid

    #208587

    Purpose

    Mammalian expression vector for expressing XBB.1.5+K478R SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation

    Depositor

    Ben Murrell

    Insert

    SARS-CoV-2 variant XBB.1.5+K478R spike (S )
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-Flag-hS1pr1-S5A_Neo

    Plasmid

    #203710

    Purpose

    Overexpression of FLAG-S1PR1-S5A mutant.

    Depositor

    Timothy Hla

    Insert

    Flag-hS1PR1-S5A (S1PR1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S5A

    Promoter

    CMV

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pALuWBB

    Plasmid

    #183202

    Purpose

    dsRed marked transgene for Crispr co-conversion with w+ gRNA and drop in cloning sites for 2 more gRNA

    Depositor

    Mitch McVey

    Insert

    w+ gRNA and scaffold followed by 2 gRNA with BbsI and BsaI cloning sites
    

    Use

    CRISPR; Recombines at attp landing site in fly ge…

    Expression

    Insect

    Promoter

    U6-3

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. PVALB mAb_BCN14.1.1A2_HC_Rat IgG2a

    Plasmid

    #182894

    Purpose

    Mammalian expression of PVALB rat mAb HC gene

    Depositor

    Heng Zhu

    Insert

    anti-PVALB (human) heavy chain (PVALB Rat)
    

    Use

    Affinity Reagent/ Antibody

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. PVALB mAb_BCN14.1.1A2_LC_Rat Ig Kappa

    Plasmid

    #182895

    Purpose

    Mammalian expression of PVALB rat mAb LC gene

    Depositor

    Heng Zhu

    Insert

    anti-PVALB (human) light chain (PVALB Rat)
    

    Use

    Affinity Reagent/ Antibody

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. TRE3GV-somBiPOLES-mKate2

    Plasmid

    #192579

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of tTA-positive neurons

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Promoter

    TRE3GV

    Available Since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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