We narrowed to 17,996 results for: puro

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pAc-sgRNA-Cas9.dora_3

Plasmid

#190702

Purpose

Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

dora (CG34401) sgRNA 3

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-sg-tdTomato166/892

Plasmid

#179919

Purpose

Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 166 and 892.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

tdTomato sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBMN-AS-FOXA1

Plasmid

#139304

Purpose

Knocking down of human FOXA1 through shRNA and amiRNA targeting FOXA1

Depositor

Claes Wadelius

Article

Pan et al iScience. 2020 Jan 9:100808. doi: 10.101

Insert

shRNA and amiRNA against FOXA1

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCF704

Plasmid

#121658

Purpose

U6-sgRNA EFS-ProCas9-Flavi-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9(C)

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCF712

Plasmid

#121663

Purpose

U6-sgRNA EF1a-ProCas9-Flavi-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9(C)

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAW12.lentiguide.GFP

Plasmid

#104374

Purpose

GFP vector for cloning in guides

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGAS-HA

Plasmid

#130910

Purpose

Expresses human cGAS-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGAS

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-KDEL_neomycin

Plasmid

#65307

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end

Use

Lentiviral

Tags

KDEL motif and Signal Sequence

Promoter

CMV

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-WT-STAT1

Plasmid

#71454

Purpose

Lentiviral expression of WT-STAT1

Depositor

George Stark

Article

Cheon et al Proc Natl Acad Sci U S A. 2009 Jun 9;106

Insert

STAT1 (STAT1 Human)

Use

Lentiviral

Tags

FLAG

Promoter

CMV

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE-SHH

Plasmid

#134350

Purpose

Targeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locus

Depositor

Lorenz Studer

Article

Cederquist et al Nat Biotechnol. 2019 Apr;37(4):436-444.

Insert

SHH (SHH Human)

Expression

Mammalian

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-Y701F-STAT1

Plasmid

#71453

Purpose

Lentiviral expression of Y701F-STAT1

Depositor

George Stark

Article

Cheon et al Proc Natl Acad Sci U S A. 2009 Jun 9;106

Insert

STAT1 (STAT1 Human)

Use

Lentiviral

Tags

FLAG

Mutation

Y701F

Promoter

CMV

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only
pHAGE-NLS-dPspCas13b-3xmRuby3-NLS

Plasmid

#132405

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Expression

Mammalian

Mutation

H133A; H1059A

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pgRNA-humanized

Plasmid

#44248

Purpose

Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

guide RNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

April 8, 2013

Availability

Academic Institutions and Nonprofits only

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pSicoR human Dicer1

Plasmid

#14763

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Dicer shRNA (DICER1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only

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pCRISPRia-v2

Plasmid

#84832

Purpose

CRISPRi/a V2 library parental plasmid

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Inserts

sgRNA
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCAIP-hXRCC1wt-eGFP

Plasmid

#206032

Purpose

Mammalian expression of hXRCC1wt coupled to eGFP under the control of a CAG promoter

Depositor

Primo Schaer

Article

Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

Insert

hXRCC1

Tags

eGFP

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO-p53-shRNA-941

Plasmid

#25637

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

Insert

p53-shRNA-941 (TP53 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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PLIN2-V5-APEX2

Plasmid

#170573

Purpose

A C-terminal APEX2 fusion to the mouse perilipin-2 protein PLIN2

Depositor

James Olzmann

Article

Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

Insert

PLIN2 (Plin2 Mouse)

Use

Lentiviral

Tags

V5-APEX2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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LZRS-IresGFP

Plasmid

#21961

Depositor

Lynda Chin

Article

Kim et al Cell. 2006 Jun 30. 125(7):1269-81.

Insert

Ires GFP

Use

Retroviral

Expression

Mammalian

Mutation

GACAGATGACTATGCAGAGAT

Available Since

Sept. 3, 2009

Availability

Academic Institutions and Nonprofits only
pAW13.lentiguide.mCherry

Plasmid

#104375

Purpose

mCherry vector for cloning in guides

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

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