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pFastBac1-ScVip1-186-1107

Plasmid

#238316

Purpose

ScVip1-186-1107 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-186-1107

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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1pBB3-ScVip1-536-1107-C793A

Plasmid

#238324

Purpose

1ScVip1-536-1107-C793A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-C793A

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-186-522

Plasmid

#238311

Purpose

ScVip1-186-522 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-186-522

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 8Y->S

Plasmid

#226606

Purpose

8 tyrosines to serine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 8Y->S (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

8 tyrosines to serine

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-H548A

Plasmid

#238319

Purpose

ScVip1-536-1107-H548A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-H548A

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107

Plasmid

#238312

Purpose

ScVip1-536-1107 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-K554A

Plasmid

#238329

Purpose

ScVip1-536-1107-K554A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-K554A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-R547A

Plasmid

#238318

Purpose

ScVip1-536-1107-R547A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-R547A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-R551A

Plasmid

#238320

Purpose

ScVip1-536-1107-R551A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-R551A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-E991A

Plasmid

#238321

Purpose

ScVip1-536-1107-E991A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-E991A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-H651A

Plasmid

#238323

Purpose

ScVip1-536-1107-H651A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-H651A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA376 - pBA904 Puro-T2A-GFP NTC guide (pRCA360 backbone)

Plasmid

#238167

Purpose

Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

Non-targeting sgRNA

Use

CRISPR and Lentiviral

Tags

GFP

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-MKI-P2A-GFP

Plasmid

#229405

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MKI-P2A-GFP

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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8_T7-PH-mScarlet3

Plasmid

#225935

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the PH membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

PH-mScarlet3

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1_dCas9-XTEN-KRAB-p2A-GFP

Plasmid

#222108

Purpose

Donor vector for CRISPRi integration at AAVS1 with a GFP fluorophore

Depositor

Jonathan Weissman

Article

She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

Insert

CRISPRi-kox1(KRAB) (cas9 Human, S. pyogenes)

Use

AAV and CRISPR

Tags

HA and P2A-GFP

Expression

Mammalian

Mutation

D10A, H840A

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCM0-109

Plasmid

#218200

Purpose

SV40 NLS for protein targeting to nucleus

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

SV40 NLS

Use

Synthetic Biology

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-858

Plasmid

#218008

Purpose

mVenus fluorescent protein

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

mVenus

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-853

Plasmid

#218011

Purpose

mCerulean fluorescent protein

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

mCerulean

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-854

Plasmid

#218012

Purpose

mVenus fluorescent protein

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

mVenus

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-855

Plasmid

#218013

Purpose

mCherry fluorescent protein

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

mCherry

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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