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pCHKU11.1-2.2

Plasmid

#115528

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4

Expression

Yeast

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCHSU71.2-2.2

Plasmid

#110685

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4

Expression

Yeast

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCHSU62.2-2.2

Plasmid

#110676

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3

Expression

Yeast

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCHSU65.2-2.2

Plasmid

#110678

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3

Expression

Yeast

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCHKU42.2-2.2

Plasmid

#110673

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3

Expression

Yeast

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCHSU70.2-2.2

Plasmid

#110683

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4

Expression

Yeast

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCHKU32.2-2.2

Plasmid

#110665

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4

Expression

Yeast

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCHSU72.2-2.2

Plasmid

#110687

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3

Expression

Yeast

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCHKU30.2-2.2

Plasmid

#110663

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4

Expression

Yeast

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pJSC119 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

Plasmid

#101207

Purpose

Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant N497A/R661A/Q695A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

N497A, R661A and Q695A

Promoter

T7

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variant

Plasmid

#101201

Purpose

Bacterial expression plasmid for SpCas9, REC3 FRET variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/S701C/S960C

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, S701C and S960C

Promoter

T7

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pJSC077 - Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

Plasmid

#101214

Purpose

Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/S355C/S867C/K855A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, S355C, S867C and K855A

Promoter

T7

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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HeFm1SpCas9

Plasmid

#92110

Purpose

Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

R661A, Q695A, K848A, Q926A, K1003A, R1060A

Promoter

Cbh

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant

Plasmid

#101200

Purpose

Bacterial expression plasmid for SpCas9, REC2 FRET variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/E60C/D273C

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, E60C and D273C

Promoter

T7

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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PatoM-OG241

Plasmid

#72842

Purpose

Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. OG241 backbone.

Depositor

Chris Barnes

Article

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoM

Expression

Bacterial

Promoter

PatoM

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP7.v2 puro

Plasmid

#87795

Purpose

Expresses an inducible short hairpin targeting human MKP7 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP7 (DUSP16 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRc2 3XFLAG-MKK4-EE-NLS

Plasmid

#87776

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK4 (MAP2K4 Human)

Tags

3xFLAG and NLS

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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KCC4_HUMAN_D0

Plasmid

#79744

Purpose

This plasmid encodes the kinase domain of KCC4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC4 (SLC12A7 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KS6A1_HUMAN_D1

Plasmid

#79720

Purpose

This plasmid encodes the kinase domain of KS6A1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KS6A1 (RPS6KA1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
STK10_HUMAN_D0

Plasmid

#79722

Purpose

This plasmid encodes the kinase domain of STK10. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

STK10 (STK10 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only