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We narrowed to 6,945 results for: pcDNA3.1+

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Showing: 4661 - 4680 of 6945 results

  3. pTBL680 CHYRON3 integration construct

    Plasmid
    #126448
    Purpose
    To integrate the CHYRON3 locus at AAVS1 in human cells.
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    pU6/3xLacO-CHYRON3 hgRNA
    pCMV-puro
    Expression
    Mammalian
    Mutation
    The SpCas9 sgRNA constant region is mutated to ma…
    Promoter
    CMV and human U6/3xLacO
    Available since
    June 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    126448-plasmid-map-sequence-id-246061

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  4. mApple-proα2(I)-L

    Plasmid
    #120161
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Publication
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    120161-plasmid-map-sequence-id-233911

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  5. mApple-proα2(I)

    Plasmid
    #119840
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with mApple between signal sequence and exon 6
    Depositor
    Sergey Leikin
    Publication
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-5 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119840-plasmid-map-sequence-id-233865

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  6. Opto-M2R

    Plasmid
    #106070
    Purpose
    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-M2R; Opto-M2R; F12; 723)
    Depositor
    Harald Janovjak
    Publication
    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Opto-M2R
    Tags
    Rhodopsin-1D4 and VSV-G
    Expression
    Mammalian
    Mutation
    Chimeric receptor protein
    Promoter
    CMV
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    106070-plasmid-map-sequence-id-209712

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  8. hHSF1 S303/7A

    Plasmid
    #118365
    Purpose
    This plasmid expresses human HSF1 S303A and S307A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.
    Depositor
    Lea Sistonen
    Publication
    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hHSF1 (HSF1 Human)
    Tags
    myc and 6xHIS
    Expression
    Mammalian
    Mutation
    S303A and S307A
    Available since
    Nov. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    118365-plasmid-map-sequence-id-227544

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  11. pcDNA-Flag1-3HABeta2-mEos2

    Plasmid
    #114187
    Purpose
    studying clustering of Beta2 receptors by superresolution microscopy (PALM)
    Depositor
    Aleksandra Radenovic
    Publication
    Annibale et al Nat Methods. 2011 Jun 12;8(7):527-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Adrenergic receptor beta 2 (ADRB2 Human)
    Tags
    3HA, FLAG, and mEOS2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    114187-plasmid-map-sequence-id-224260

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  12. PH-dL5**

    Plasmid
    #96946
    Purpose
    PLC pleckstrin homology domain linked to FAP for CALI validation
    Depositor
    Marcel Bruchez
    Publication
    He et al Nat Methods. 2016 Mar;13(3):263-268. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Pleckstrin homology domain
    Tags
    dL5 and mCerulean
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    96946-plasmid-map-sequence-id-210240

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  14. pTE4999

    Plasmid
    #107555
    Purpose
    Expresses human codon optimized inactive FnCpf1(dead1) in mammalian cells.
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hFnCpf1(dead1)
    Use
    CRISPR
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    D917A
    Promoter
    CMV
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    107555-plasmid-map-sequence-id-207200

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  18. VN-SOD1

    Plasmid
    #89757
    Purpose
    expresses human SOD1 fused to the N-terminal part of Venus
    Depositor
    Tiago Outeiro
    Publication
    Brasil et al Proc Natl Acad Sci U S A. 2019 Dec 17;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SOD1 (SOD1 Human)
    Tags
    N-terminal part of Venus
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    89757-plasmid-map-sequence-id-178830

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  19. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid
    #61364
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61364-plasmid-map-sequence-id-99800

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  20. VfAU1-LOV_136

    Plasmid
    #63632
    Purpose
    VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) preceded by a restriction site
    Depositor
    Harald Janovjak
    Insert
    VfAU1-LOV
    Expression
    Mammalian
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63632-plasmid-map-sequence-id-108793

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Showing: 4661 - 4680 of 6945 results