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pGST-TEV-yVms1(1-417,delta(38-69))

Plasmid

#128739

Purpose

Bacterial overexpression and crystallization

Depositor

Chris Hill

Article

Nielson et al Mol Cell. 2017 Nov 16;68(4):673-685.e6.

Insert

Vms1 (VCP/Cdc48-associated mitochondria stress-responsive 1) (VMS1 Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

residues 1-417 with deletion of 38-69

Promoter

T7

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pET-21a_2xNLS_FnCpf1_MBP_protein_expression

Plasmid

#120801

Purpose

Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

2xNLS_FnCpf1

Use

CRISPR

Tags

MBP and NLS

Expression

Bacterial

Promoter

T7

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pL59-Nat

Plasmid

#140465

Purpose

CRISPR/Cas9 engineering with nourseothricin selection

Depositor

Gianni Liti

Article

Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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M26V DJ-1 pET15b

Plasmid

#60688

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26V DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

M26V

Promoter

T7

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pWJPrP5

Plasmid

#22106

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

mouse PrP ORF insert (Prnp Mouse)

Use

Construction of targeting vector

Mutation

mutated codon 177 (D to N)

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only
pCas9_antiBFP

Plasmid

#220922

Purpose

Expresses Cas9 with a BFP targeting crRNA for use in targeting the BFP plasmid in a cell based assay

Depositor

Ryan Jackson

Article

Walters et al J Microbiol Biol Educ. 2026 Apr 30;27(1)

Insert

pCas9

Expression

Bacterial

Available Since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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pCas9_antiGFP

Plasmid

#220923

Purpose

Expresses Cas9 with a GFP targeting crRNA for use in targeting the GFP plasmid in a cell based assay

Depositor

Ryan Jackson

Article

Walters et al J Microbiol Biol Educ. 2026 Apr 30;27(1)

Insert

pCas9

Expression

Bacterial

Available Since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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pCas9_antiOFP

Plasmid

#220924

Purpose

Expresses Cas9 with a OFP targeting crRNA for use in targeting the OFP plasmid in a cell based assay

Depositor

Ryan Jackson

Article

Walters et al J Microbiol Biol Educ. 2026 Apr 30;27(1)

Insert

pCas9

Expression

Bacterial

Available Since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-sgStuffer-GFP-Puro

Plasmid

#208349

Purpose

Lentivirus sgRNA backbone with GFP and PuroR. Compatible with golden gate cloning (Esp3i/BsmBI)

Depositor

Peter S Nelson

Article

Frank et al eLife 2025

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-sgStuffer-mCherry-Puro

Plasmid

#208350

Purpose

Lentivirus sgRNA backbone with mCherry and PuroR. Compatible with golden gate cloning (Esp3i/BsmBI)

Depositor

Peter S Nelson

Article

Frank et al eLife 2025

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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