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Plasmid#17291Purpose3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, HygroDepositorTypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only
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pLG011 (DRT type 3)
Plasmid#157889PurposeExpresses the DRT type 3 defense systemDepositorInsertDRT type 3 (RT + RT)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
3TERM_Nos-35S
Plasmid#136116Purpose3TERM after CDS/ CDS12-CTAGDepositorInsert3TERM_Nos-35S (A. tumefaciens, CaMV)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMpGWB203
Plasmid#68594PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
PROM5_MpEF1a
Plasmid#136113PurposeStrong promoter from MpEF1a, that drives expression everywhere in Mp gemmae but stronger in the notchesDepositorInsertPROM5_MpEF1a Mapoly0024s0116
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLIC-SM-Myc
Plasmid#111189PurposeHigh affinity Myc tagging of weakly expressed genes (optimized for Toxoplasma gondii)DepositorTypeEmpty backboneUseToxoplasma gondii expressionTagsSpaghetti Monster Myc TagExpressionMutationPromoterEndogenousAvailable sinceAug. 2, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDestTol2pA2-U6:gRNA
Plasmid#63157PurposeDestination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.DepositorTypeEmpty backboneUseCRISPR; Tol2 destination vector for gatewayTagsExpressionMutationPromoterAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti-entry-puro
Plasmid#85745PurposeLentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
mNG-sfTq2ox
Plasmid#124219PurposeCytoplasmic mNeongreen superfolder mTurquoise2 ox tandem, ~60 % energy transfer. pNM045 - pSAV057-mNG-sfTq2oxDepositorInsertmNG-sfTq2ox
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
2HA-pDEST-C
Plasmid#118374PurposeGateway compatible mammalian expression vector with C-terminal 2HA to tag proteins of interest.DepositorTypeEmpty backboneUseDestinationTags2HAExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDestattB/CY (pCM327)
Plasmid#68341PurposeMultisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish, contains an attB site and alpha-cystallin:YFP (CY) transgenesis marker in backbone.DepositorTypeEmpty backboneUseZebrafish transgenesisTagsExpressionMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLIC-SM-HA
Plasmid#111188PurposeHigh affinity HA Tagging of weakly expressed genes (optimized for Toxoplasma gondii)DepositorTypeEmpty backboneUseToxoplasma gondii expressionTagsSpaghetti Monster HA TagExpressionMutationPromoterEndogenousAvailable sinceAug. 2, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMRE162
Plasmid#118510Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCKmatBC
Plasmid#138587PurposematBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, catDepositorInsertbicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
UseSynthetic BiologyTagsExpressionBacterialMutationmatB contains native GUG start codonPromoterPlac promoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZa pBAD csgA CBDcex pLac Ara csgGEF
Plasmid#226378PurposePlasmid carries csgA and csgG,E,F genes of curli operonDepositorInsertcsgA and csgG,E,F genes of curli operon
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGAN230
Plasmid#131122Purposereverse yTRAP, RNQ1-aTF reporting on tetR-mKate2DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterSUP35Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRPF215
Plasmid#106377PurposeC. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposonDepositorInsertPtet-himar1-Ter(slpA)-transposon
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationCodon optimised for C. difficilePromoterPtetAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLG012 (DRT type 4)
Plasmid#157890PurposeExpresses the DRT type 4 defense systemDepositorInsertDRT type 4
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAJT195
Plasmid#202294PurposeType IIS cloning plasmid for LPattB7 integrations w/ OriTDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTE5400_pMBP_CDS dropout vector
Plasmid#221189PurposeExpression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interestDepositorTypeEmpty backboneUseTags10x His-tag + E. coli maltose binding protein (MB…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only