We narrowed to 21,861 results for: Tes

1. Flag-NLS-MaSat ZF-18-mVenusN

   Plasmid

   #191392

   Purpose

   Zinc finger fused to N-terminal part of split mVenus

   Depositor

   Albert Jeltsch

   Article

   Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

   Insert

   Flag-NLS-MaSat ZF-18-mVenusN
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLV hUbC-Cas9-P2A-Puro_ST-sgRNA

   Plasmid

   #188705

   Purpose

   Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human safe targeting loci

   Depositor

   David Tobin

   Article

   Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

   Insert

   ST sgRNAs
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. UAS-GFP::NMN-Deamidase

   Plasmid

   #187868

   Purpose

   Gal4/UAS expression of GFP::NMN-Deamidase

   Depositor

   Lukas Neukomm

   Article

   Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

   Insert

   NMN-Deamidase
   

   Use

   Tags

   GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCRISPR3_BC6

   Plasmid

   #191861

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CCTAGT) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCRISPR3_BC5

   Plasmid

   #191860

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCRISPR3_BC4

   Plasmid

   #191859

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCRISPR3_BC3

   Plasmid

   #191858

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCRISPR3_BC1

   Plasmid

   #191856

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGAL1-VP1 (LEU2)

   Plasmid

   #182480

   Purpose

   Yeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains leucine auxotrophic marker (K. lactis LEU2).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Insert

   deltaVP1
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mRuby3 only VLP

    Plasmid

    #182429

    Purpose

    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and mRuby3 tagged with VP2C (GAL10 promoter). mRuby3 was codon-optimised for E. coli. Contains uracil marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-mRuby3
    deltaVP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. LLP728_L2_pV01_0I_FRT-TB-rLUC_TCTP-fLUC

    Plasmid

    #192371

    Purpose

    To test for the repressive potential of the Transcription Block (TB) with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-TB-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pENT-L3-eBra-L4

    Plasmid

    #186368

    Purpose

    Entry clone with ORF encoding Brachury cis-regulatory region flanked by Gateway recombination sequences

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    Brachury notochord enhancer
    

    Use

    Protein expression

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pENT-L3-Titf-L4

    Plasmid

    #186367

    Purpose

    Entry clone with ORF encoding Titf1 cis-regulatory region flanked by Gateway recombination sequences

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    Titf endoderm enhancer
    

    Use

    Protein expression

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pENT-L3-pSnail-L5

    Plasmid

    #186365

    Purpose

    Entry clone with ORF encoding Ci-Snail cis-regulatory region flanked by Gateway recombination sequences

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    Snail regulatory sequence
    

    Use

    Protein expression

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Full-length WT talin1

    Plasmid

    #182992

    Purpose

    express FL WT talin1 in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    Talin1 WT (Tln1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Full-length P229S talin1

    Plasmid

    #182993

    Purpose

    express FL variant P229S talin1 in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    Talin1 P229S (Tln1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    P229S

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT7-V5-SBP-C1-DmRoquin-ILLVtoEEEE_AE

    Plasmid

    #148636

    Purpose

    Mammalian Expression of DmRoquin-ILLVtoEEEE

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    DmRoquin-ILLVtoEEEE (roq Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT7-V5-SBP-C1-HsRoquin1_AE

    Plasmid

    #148637

    Purpose

    Mammalian Expression of HsRoquin1

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    HsRoquin1 (RC3H1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT7-V5-SBP-C1-HsRoquin1_501-1133_AE

    Plasmid

    #148638

    Purpose

    Mammalian Expression of HsRoquin1_501-1133

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    HsRoquin1_501-1133 (RC3H1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7-V5-SBP-C1-HsRoquin2_AE

    Plasmid

    #148639

    Purpose

    Mammalian Expression of HsRoquin2

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    HsRoquin2 (RC3H2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pT7-V5-SBP-C1-HsRoquin2_1-500_AE

    Plasmid

    #148640

    Purpose

    Mammalian Expression of HsRoquin2_1-500

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    HsRoquin2_1-500 (RC3H2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pT7-V5-SBP-C1-HsRoquin2_501-1191_AE

    Plasmid

    #148641

    Purpose

    Mammalian Expression of HsRoquin2_501-1191

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    HsRoquin2_501-1191 (RC3H2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pT7-V5-SBP-C1-HsRoquin_1-500_AE

    Plasmid

    #148642

    Purpose

    Mammalian Expression of HsRoquin1_1-500

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    HsRoquin1_1-500 (RC3H1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pT7-V5-SBP-C1-DmRoquin_AD

    Plasmid

    #148535

    Purpose

    Mammalian Expression of DmRoquin

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    DmRoquin (roq Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pT7-V5-SBP-C1-DmRoquin_1-500_AD

    Plasmid

    #148536

    Purpose

    Mammalian Expression of DmRoquin_1-500

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    DmRoquin_1-500 (roq Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pT7-V5-SBP-C1-DmRoquin_501-819_AD

    Plasmid

    #148537

    Purpose

    Mammalian Expression of DmRoquin_501-819

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    DmRoquin_501-819 (roq Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pENT-R4-bpFOG-L5

    Plasmid

    #186369

    Purpose

    Entry clone with ORF encoding Strong basal promoter of FOG flanked by Gateway recombination sequences

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    Basal promoter of the Friend Of Gata gene
    

    Use

    Protein expression

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pENT-L3-pFOGl-L5

    Plasmid

    #186364

    Purpose

    Entry clone with ORF encoding Ci-FOG cis-regulatory region flanked by Gateway recombination sequences

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    Friend Of Gata regulatory sequence
    

    Use

    Protein expression

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSEVA251::PpsbA2*::B0032::CYP110D1

    Plasmid

    #186707

    Purpose

    CYP110D1 coding sequence under the control of PpsbA2* promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

    Depositor

    Paula Tamagnini

    Article

    Mascia et al Green Chemistry, 24 (2022):6156

    Insert

    alr4766
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PpsbA2*

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSEVA251::Ptrc.x.tetO2::B0032::CYP110D1

    Plasmid

    #186709

    Purpose

    CYP110D1 coding sequence under the control of Ptrc.x.tetO2 promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

    Depositor

    Paula Tamagnini

    Article

    Mascia et al Green Chemistry, 24 (2022):6156

    Insert

    alr4766
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Ptrc.x.tetO2

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge