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Plasmid#201535PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE_RBS3_CB4
UseSynthetic BiologyTagsExpressionMutationrhaS ΔM1-H5, PrhaSR Δ179bpPromoterPrhaBAD (rhamnose)Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSC5GGv1_ShBle
Plasmid#188603PurposepSC5GGv1 with Sh ble insert for yeast selectionDepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C3
Plasmid#189620PurposeCloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertReduced Mitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationRemoved repeat region, 10-kbp deletion, and minor…PromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-1700029I15Rik-3×FLAG
Plasmid#199689PurposeExpression vector of mouse 1700029I15Rik. 3XFLAG tag at C-terminus.DepositorInsert1700029I15Rik (1700029I15Rik Mouse)
UseTags3xFLAGExpressionMammalianMutationPromoterCAGAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-KIF3B tail 391-747
Plasmid#193929PurposeMammalian expression of Halo-tagged Kif 3B tail 391-747DepositorInsertKif 3B (Kif3b Mouse)
UseTagsHaloExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
M2C5
Plasmid#188606PurposeMinimal Conjugative Plasmid 2DepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
M1C1
Plasmid#188609PurposeMinimal Conjugative Plasmid 1DepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGST-parallell2-GGA3(571-723)
Plasmid#199150PurposeExpresses GST-GGA3(571-723) in bacteriaDepositorInsertGGA3(571-723) (GGA3 Human)
UseTagsGST and TEV protease cleavage siteExpressionBacterialMutationPromotertacAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-A10
Plasmid#196081PurposeInducible CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.DepositorInsertgRNA targeting Adam10
UseCRISPRTagsExpressionMammalianMutationCloned using SapI sites - destroyed after inserti…PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-A10
Plasmid#196083PurposeInducible CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.DepositorInsertgRNA targeting Adam10
UseCRISPRTagsExpressionMammalianMutationCloned using SapI sites - destroyed after inserti…PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-A10
Plasmid#196075PurposeConstitutive CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.DepositorInsertgRNA targeting Adam10
UseCRISPRTagsExpressionMammalianMutationCloned using SapI sites - destroyed after inserti…PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-A10
Plasmid#196077PurposeConstitutive CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.DepositorInsertgRNA targeting Adam10
UseCRISPRTagsExpressionMammalianMutationCloned using SapI sites - destroyed after inserti…PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas4_1_Δ2_VA
Plasmid#186439PurposeEncodes Cas4, Cas1, leader, repeat and one spacer from F. Novicida (type V-A)DepositorInsertCas4, Cas1 and Cas2
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCasΔ4_1_2_VA
Plasmid#186440PurposeEncodes Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)DepositorInsertCas1 and Cas2
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas-mut4_1_2_VA
Plasmid#186441PurposeEncodes Cas4 (K70A), Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)DepositorInsertCas4, Cas1 and Cas2
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas-4/1_2_VB
Plasmid#186442PurposeEncodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)DepositorInsertCas4/1 and Cas2
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas-4/1Δ2_VB
Plasmid#186443PurposeEncodes Cas4/1, leader, repeat and one spacer from A. acidoterrestris (type V-B)DepositorInsertCas4/1
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas12(PI)
Plasmid#186448PurposeEncodes inactive Cas12a containing mutation in the PI domain (K613A, K617A) from F. novicida (type V-A)DepositorInsertCas12a (PI)
UseCRISPRTagsExpressionMutationK613A, K617APromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas12b
Plasmid#186449PurposeEncodes Cas12b from A. acidoterrestris (type V-B)DepositorInsertCas12b
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas12b(PI)
Plasmid#186451PurposeEncodes inactive Cas12b containing mutation in the PI domain (R122A, G143P) from A. acidoterrestris (type V-B)DepositorInsertCas12b (PI)
UseCRISPRTagsExpressionMutationR122A, G143PPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only