We narrowed to 21,861 results for: Tes

1. FnRPR9-10

   Plasmid

   #182568

   Purpose

   Human fibronectin fragment, type-III repeats 9-10, R1374A, P1376A, R1379A mutant

   Depositor

   Timothy Springer

   Article

   Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

   Insert

   Human fibronectin fragment, type-III repeats 9-10
   

   Use

   Tags

   His and TEV cleavage site after His tag

   Expression

   Bacterial

   Mutation

   R1374A, P1376A, R1379A

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. FnRPR8-10

   Plasmid

   #182570

   Purpose

   Human fibronectin fragment, type-III repeats 8-10, R1374A, P1376A, R1379A mutant

   Depositor

   Timothy Springer

   Article

   Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

   Insert

   Human fibronectin fragment, type-III repeats 8-10
   

   Use

   Tags

   His and TEV cleavage site after His tag

   Expression

   Bacterial

   Mutation

   R1374A, P1376A, R1379A

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET_His6-MBP-TEV-Cas6-RT-Cas1_WT

   Plasmid

   #186274

   Purpose

   Expresses Thiomicrospira Cas6-RT-Cas1 in E. coli

   Depositor

   Jennifer Doudna

   Article

   Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

   Insert

   Thiomicropira Cas6-RT-Cas1
   

   Use

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PlmCas12e

   Plasmid

   #188496

   Purpose

   Expresses FLAG tagged PlmCas12e

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas12e
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENT-L3-a-element-L4

   Plasmid

   #186366

   Purpose

   Entry clone with ORF encoding Otx cis-regulatory region flanked by Gateway recombination sequences

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   a-element early Otx neural enhancer
   

   Use

   Protein expression

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono3

   Plasmid

   #73542

   Purpose

   Expression of sgRNA targeting LINE-1 and TagBFP

   Depositor

   Constance Ciaudo

   Article

   Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

   Insert

   LINE-1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono1

   Plasmid

   #73543

   Purpose

   Expression of sgRNA targeting LINE-1 and TagBFP

   Depositor

   Constance Ciaudo

   Article

   Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

   Insert

   LINE-1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-T11HA-hMOV10-CD

   Plasmid

   #178908

   Purpose

   expression of catalytic dead hMOV10 K530A

   Depositor

   Constance Ciaudo

   Article

   Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

   Insert

   cDNA Catalytic dead hMOV10 (MOV10 Human)
   

   Use

   Tags

   T11 beta strand of GFP-HA

   Expression

   Mammalian

   Mutation

   K530A catalytic dead AAG>GCA

   Promoter

   CMV protmoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCfB9341 (pgRNA_IX-1_NatMX)

   Plasmid

   #161593

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IX-1

   Depositor

   Irina Borodina

   Article

   Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

   Insert

   guiding RNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCfB9343 (pgRNA_XV-1_NatMX)

    Plasmid

    #161595

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XV-1

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Insert

    guiding RNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCfB9358(VII-1_Markerfree_BackBone)

    Plasmid

    #161599

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VII-1, (Chr VII: 438509..438490)

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCfB9359(VIII-1_Markerfree_BackBone)

    Plasmid

    #161600

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VIII-1, (Chr VIII: 293615..293634)

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCfB9361(XIII-1_Markerfree_BackBone)

    Plasmid

    #161602

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XIII-1, (Chr XIII: 306804..306823)

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCfB9363(XVI-1_Markerfree_BackBone)

    Plasmid

    #161604

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XVI-1, (Chr XVI: 406267..406286)

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ppwd1.473.646.W601A

    Plasmid

    #137660

    Purpose

    Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

    Depositor

    Melissa Jurica, Tara Davis

    Article

    Adams et al Biochem J. 2015 Jul 15;469(2):223-33. do

    Insert

    ppwd1 (PPWD1 Human)
    

    Use

    Tags

    (His)6-thrombin

    Expression

    Bacterial

    Mutation

    473-646-W601A

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Phat2-dr-cPla2-C2-D34R-D87R-EGFP-6H

    Plasmid

    #187188

    Purpose

    Protein Expression

    Depositor

    Philipp Niethammer

    Article

    Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

    Insert

    ENSDARG00000024546 (pla2g4aa Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    D34R, D87R

    Promoter

    Available since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Phat2-dr-cPla2-C2-L32F-L33F-EGFP-6H

    Plasmid

    #187184

    Purpose

    Protein Expression

    Depositor

    Philipp Niethammer

    Article

    Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

    Insert

    ENSDARG00000024546 (pla2g4aa Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    L32F, L33F

    Promoter

    Available since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Phat2-dr-cPla2-C2-Y90A-V91A-EGFP-6H

    Plasmid

    #187185

    Purpose

    Protein Expression

    Depositor

    Philipp Niethammer

    Article

    Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

    Insert

    ENSDARG00000024546 (pla2g4aa Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Y90A, V91A

    Promoter

    Available since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Phat2-dr-cPla2-C2-L32A-L33A-EGFP-6H

    Plasmid

    #187183

    Purpose

    Protein Expression

    Depositor

    Philipp Niethammer

    Article

    Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

    Insert

    ENSDARG00000024546 (pla2g4aa Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    L32A, L33A

    Promoter

    Available since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Phat2-dr-Alox12-PLAT-EGFP-6H

    Plasmid

    #187190

    Purpose

    Protein Expression

    Depositor

    Philipp Niethammer

    Article

    Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

    Insert

    ENSDARG00000069463 (alox12 Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Phat2-hs-PKCgamma-C2-EGFP-6H

    Plasmid

    #187115

    Purpose

    Protein Expression

    Depositor

    Philipp Niethammer

    Article

    Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

    Insert

    ENSG00000126583 (PRKCG Human)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Phat2-dr-cPla2-C2-D34K-D87K-EGFP-6H

    Plasmid

    #187187

    Purpose

    Protein Expression

    Depositor

    Philipp Niethammer

    Article

    Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

    Insert

    ENSDARG00000024546 (pla2g4aa Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    D34K, D87K

    Promoter

    Available since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. membrane-HsPLK1-dead

    Plasmid

    #185572

    Purpose

    to see protein dynamics

    Depositor

    Mamiko Yajima

    Article

    Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

    Insert

    PLK1-dead (PLK1 Human)
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    membrane targeting sequence from Neuromodulin (ML…

    Expression

    Mutation

    K82R kinase-dead mutant

    Promoter

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. FAK C-term

    Plasmid

    #186147

    Purpose

    For expresseion of recombinant FAK c-terminal domain in E. coli

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Crimson/P2A-Control (pSLIK3)

    Plasmid

    #113858

    Purpose

    An isogenic synaptase-free control vector to evaluate recombination using endogenous functions for N-terminal Crimson/P2A fusion plasmids.

    Depositor

    Richard S Myers

    Article

    Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

    Insert

    E2-Crimson
    

    Use

    Lentiviral

    Tags

    P2A/Crimson

    Expression

    Mutation

    Promoter

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TRE-RARa-VP16

    Plasmid

    #185561

    Purpose

    Doxycycline inducible expression of constitutively active Retinoic Acid Receptor alpha

    Depositor

    Connie Cepko

    Article

    Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:

    Insert

    RARa-VP16 (Rara Mouse)
    

    Use

    Tags

    VP16

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MSCV-Arkadia

    Plasmid

    #180372

    Purpose

    expressing mouse Arkadia proteins

    Depositor

    Dan Littman

    Article

    Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

    Insert

    Arkadia (Rnf111 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR promoter

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p8291 MSCV-Neo C-HA 18E7 ΔDLLC

    Plasmid

    #184523

    Purpose

    Expression of HPV18 E7 ΔDLLC

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

    Insert

    HPV18 E7 ΔDLLC
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    deletion of DLLC

    Promoter

    MSCV LTR

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEF1α -NWM-Node 1

    Plasmid

    #154253

    Purpose

    expresses reconstructed New World monkey retroCHMP3 Node 1 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

    Insert

    reconstructed Node 1 of New World monkey retroCHMP3
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEF1α -NWM-Node 2

    Plasmid

    #154254

    Purpose

    expresses reconstructed New World monkey retroCHMP3 Node 2 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

    Insert

    reconstructed Node 2 of New World monkey retroCHMP3
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge