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pcDNA3.1-CMV-Nier1b-3NLS-P2A-EGFP-3NLS

Plasmid

#217479

Purpose

Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1b

Depositor

Peng Zou

Article

Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

Insert

Nier1b

Tags

3xNLS, P2A-EGFP-3xNLS

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMamCol1

Plasmid

#215184

Purpose

Allows for stable expression of recombinant (tagged/untagged) proteins in AAVS1 locus of Human cells and transient Protein Expression in E. coli

Depositor

Krishna Kishore Inampudi

Insert

tsPurple

Use

AAV, CRISPR, and Synthetic Biology ; Recombinant …

Tags

SNAC-StrepII tag

Expression

Bacterial and Mammalian

Promoter

TetON 3G Bidirectional for Mammalian and Trc with…

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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MCP-YPet

Plasmid

#215747

Purpose

Expresses MS2 coat protein (MCP) fused to YPet

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

YPet

Expression

Mammalian

Promoter

EF1a

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB602-po-mCerulean

Plasmid

#213861

Purpose

Binary vector for the expression of peroxisome transit peptide fused mCerulean in plants (for Agrobacterium-mediated genetic transformation and visualization of peroxisome.)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

Insert

Peroxisome transit peptide fused mCerulean

Expression

Bacterial and Plant

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST14-Cys-SpyCatcher (Min)

Plasmid

#216713

Purpose

Expresses truncated minimal form of SpyCatcher with free cysteine for labeling in bacteria. See also Li...Howarth, J Mol. Biol., 2014

Depositor

Alex Dunn

Article

Zhong et al Cell Rep Methods. 2022 Sep 2;2(9):100278

Insert

Cys-SpyCatcher (Min)

Tags

6xHis

Expression

Bacterial

Mutation

See Li...Howarth, J. Mol. Biol., 2014

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pFH2.18_TagBFP

Plasmid

#205533

Purpose

Express TagBFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

TagBFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLipoP1-NLS-3xmCitrine

Plasmid

#192522

Purpose

Fluorescent reporter for plant responses to bacterial infection

Depositor

Gitta Coaker

Article

Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

Insert

Putative membrane lipoprotein (AT3G18250 Mustard Weed)

Tags

3xmCitrine and NLS

Expression

Plant

Promoter

LipoP1

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-ASAP3ER

Plasmid

#195358

Purpose

ASAP3ER under control of human synapsin promoter

Depositor

Brenda Bloodgood

Article

Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

Insert

ASAP3ER

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

human synapsin

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pPB UbC Arrestin-uTEV1-V5; UAS-mCherry

Plasmid

#173122

Purpose

Expresses uTEV1 and reporter receiver construct in DIPG

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

Arrestin-uTEV1-V5; UAS-mCherry

Expression

Mammalian

Promoter

UbC

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pPB EF1a CCR6-hLOV-TEVcs-Gal4

Plasmid

#173120

Purpose

Expresses CCR6 hLOV receiver construct in DIPG

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCR6-hLOV-TEVcs-Gal4

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pPB UbC Arrestin-TEVp(WT)-V5; UAS-mCherry

Plasmid

#173121

Purpose

Expresses TEVp(WT) and reporter receiver construct in DIPG

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

Arrestin-TEVp(WT)-V5; UAS-mCherry

Expression

Mammalian

Promoter

UbC

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g1_CASH-1

Plasmid

#188975

Purpose

A human codon-optimized SpCas9 nickase and chimeric g1 guide RNA expression plasmid.

Depositor

Sangdun Choi

Article

Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

Insert

g1 guide RNA

Expression

Mammalian

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g2_CASH-1

Plasmid

#188976

Purpose

A human codon-optimized SpCas9 nickase and chimeric g2 guide RNA expression plasmid.

Depositor

Sangdun Choi

Article

Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

Insert

g2 guide RNA

Expression

Mammalian

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Plekhh1-FERM

Plasmid

#187371

Purpose

Expresses human Plekhh1-FERM labelled with EGFP

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

Insert

Plekhh1-FERM

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Plekhh1-CTer

Plasmid

#187369

Purpose

Expresses human Plekhh1-CTer labelled with EGFP

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

Insert

Plekhh1-CTer

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Plekhh1-MyTH-FERM

Plasmid

#187370

Purpose

Expresses human Plekhh1-MyTH-FERM labelled with EGFP

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

Insert

Plekhh1-MyTH-FERM

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Plekhh1-NTer

Plasmid

#187368

Purpose

Expresses human Plekhh1-NTer labelled with EGFP

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

Insert

Plekhh1-NTer

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Myo1-IQ-Tail

Plasmid

#187363

Purpose

Expresses rat myosin 1b isoform b IQTail domain labelled with EGFP

Depositor

Evelyne Coudrier

Article

Raposo et al Mol Biol Cell. 1999 May . 10(5):1477-94.

Insert

myosin 1b isoform b IQTail domain (Myo1b Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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CcCDP_p15A

Plasmid

#179272

Purpose

Expresses CcCDP in E. coli BL21(DE3)

Depositor

Bernd Nidetzky

Article

Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

Insert

cellodextrin phosphorylase

Use

Synthetic Biology

Tags

6xHIS

Expression

Bacterial

Promoter

T7 lacO

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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Xalb_I-F1_array_GG

Plasmid

#178770

Purpose

Golden Gate vector to generate X. albilineans type I-F1 single arrays

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

Golden Gate vector BaeI recognition site to generate X. albilineans type I-F1 single arrays

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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