We narrowed to 9,127 results for: Pol

1. pBP-T7_RBS

   Plasmid

   #72978

   Purpose

   T7 promoter and RBS - derived from pET3a (5' CTAT/3' CATA) fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   T7+RBS
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBP-lacZ

   Plasmid

   #72948

   Purpose

   Entry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloning

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 0 - Bioparts
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTU2-A-lacZ

   Plasmid

   #72950

   Purpose

   Level 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 2 - A site
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTU2-B-lacZ

   Plasmid

   #72951

   Purpose

   Level 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 2 - B site
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTU2-C-lacZ

   Plasmid

   #72952

   Purpose

   Level 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 2 - C site
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTU2-D-lacZ

   Plasmid

   #72953

   Purpose

   Level 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 2 - D site
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTU2-B-RFP

   Plasmid

   #72955

   Purpose

   Level 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 2 - B site
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTU2-C-RFP

   Plasmid

   #72956

   Purpose

   Level 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 2 - C site
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTU2-D-RFP

   Plasmid

   #72957

   Purpose

   Level 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 2 - D site
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTU2-b-RFP

    Plasmid

    #72959

    Purpose

    Level 2 Destination vector - Position B - Assembly of 3 TU's from Level 1-ABC

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - b site
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

11. CAG-mCherry-RORbshRNA2

    Plasmid

    #73984

    Purpose

    RORb shRNA2 (mir155 backbone) was inserted into the 3'UTR of mCherry.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    shRNA that targets the mouse RORb gene
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CAG-EGFP-RORbshRNA1

    Plasmid

    #73985

    Purpose

    RORb shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    shRNA that targets the mouse RORb gene
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CAG-EGFP-RORbshRNA2

    Plasmid

    #73986

    Purpose

    RORb shRNA2 (mir155 backbone) was inserted into the 3'UTR of EGFP.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    shRNA that targets the mouse RORb gene
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CAG-mCherry-Otx2shRNA2

    Plasmid

    #73980

    Purpose

    Otx2 shRNA2 (mir155 backbone) was inserted into the 3'UTR of mCherry.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    shRNA that targets the mouse Otx2 gene
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCellFree_G03 ZFYVE9

    Plasmid

    #67134

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    ZFYVE9 (ZFYVE9 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Mutation

    F1359I

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pCGS652

    Plasmid

    #62220

    Purpose

    Mammalian expression TALEN vector with N152, C+18 truncations

    Depositor

    Daniel Voytas

    Article

    Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

    Type

    Empty backbone

    Use

    TALEN

    Tags

    AcV5 and SV40 NLS

    Expression

    Mammalian

    Promoter

    miniCaggs

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET:CACT:GFP

    Plasmid

    #60737

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CACT' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RP185 pUAST EGFP:Or47b

    Plasmid

    #59525

    Purpose

    N-terminal fusion of GFP to Drosophila melanogaster Or47b

    Depositor

    Leslie Vosshall

    Article

    Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

    Insert

    EGFP:Or47b (Or47b Fly)
    

    Tags

    EGFP

    Expression

    Insect

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

19. Myc-CICf_AEA

    Plasmid

    #48188

    Purpose

    Expresses AEA mutated Cic

    Depositor

    Huda Zoghbi

    Article

    Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

    Insert

    Capicua (Cic Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-UNC60B

    Plasmid

    #48740

    Purpose

    Bacterial expression of UNC-60B (a C. elegans ADF/cofilin)

    Depositor

    Shoichiro Ono

    Article

    Ono et al J Biol Chem. 1998 Feb 6;273(6):3778-83.

    Insert

    C. elegans UNC-60B cDNA (unc-60 Nematode)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 1, 2013

    Availability

    Academic Institutions and Nonprofits only