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pMRS-AraC-dCas9

Plasmid

#220195

Purpose

mini-Tn7 dCas9 expression vector - AraC/pBAD promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9

Use

CRISPR

Expression

Bacterial

Mutation

Pseudomonas aeruginosa codon optimized

Promoter

AraC/pBAD

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMRS-BG35-GFP

Plasmid

#220201

Purpose

mini-Tn7 GFP expression vector - pBG35 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

monomeric superfolder green fluorescent protein

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG35

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-pDisplay-SNAP[C145A]

Plasmid

#223496

Purpose

Expression of dead-mutant SNAP-tags to outer surface in mammalian cells

Depositor

James Frank

Article

Tobias et al Chem Sci. 2021 Sep 15;12(40):13506-13512

Insert

dead-mutant SNAP-tag

Expression

Mammalian

Mutation

SNAP-tag C145A

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPU-HSA-miR30-MDA5

Plasmid

#174222

Purpose

All-in-one control rescue-MDA5 shRNA knockdown vector

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

HSA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Tol2_-87prox1a:basEGFP_Acry:GFP

Plasmid

#218213

Purpose

Zebrafish -87prox1a enhancer reporter in the lymphatics at 5 dpf. Acry_GFP as a control in the lens

Depositor

Katarzyna Koltowska

Article

Panara et al Development. 2024 May 1;151(9):dev202525

Insert

-87prox1a

Use

Zebrafish expression

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pWzyE-His

Plasmid

#196669

Purpose

Expression of C-terminally His10-tagged WzyE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzyE

Tags

TEV cleavable His10

Expression

Bacterial

Promoter

araBAD

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL391

Plasmid

#219501

Purpose

Express Ade2 and Faa1 editrons from a single Gal7 promoter, separated by a Csy4 recognition sequence

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

Eco1 editing ncRNAs and Cas9 gRNAs to edit ADE2 and FAA1

Expression

Yeast

Promoter

GAL7

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-Crym-eGFP

Plasmid

#200080

Purpose

To study u-crystallin protein interactors, control plasmid

Depositor

Baljit Khakh

Article

Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

Insert

GfaABC1D-Crym-eGFP

Use

AAV and Mouse Targeting

Tags

eGFP

Promoter

GfaABC1D

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFPnls barcode-210-SV40 polyA

Plasmid

#190876

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFPnls-barcode210

Use

AAV

Mutation

WT

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.H10L

Plasmid

#198683

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XI1_UP

Use

Synthetic Biology

Expression

Yeast

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.H10R

Plasmid

#198684

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XI1_DW

Use

Synthetic Biology

Expression

Yeast

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.H09R

Plasmid

#198681

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XII4_UP

Use

Synthetic Biology

Expression

Yeast

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.H09L

Plasmid

#198682

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XII4_DW

Use

Synthetic Biology

Expression

Yeast

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.H06R

Plasmid

#198676

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XII5_DW

Use

Synthetic Biology

Expression

Yeast

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.H03L

Plasmid

#198669

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XII2_UP

Use

Synthetic Biology

Expression

Yeast

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.H01R

Plasmid

#198666

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

X3_DW

Use

Synthetic Biology

Expression

Yeast

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/sh-FYN1-GFP_Seq_C

Plasmid

#201399

Purpose

Knockdown of FYN tyrosine kinase. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Neuro Oncol. 2020 Jun 9;22(6):806-818. d

Insert

FYN

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.H11R

Plasmid

#198686

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XII1_DW

Use

Synthetic Biology

Expression

Yeast

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.H11L

Plasmid

#198685

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XII1_UP

Use

Synthetic Biology

Expression

Yeast

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.H08R

Plasmid

#198680

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XI5_DW

Use

Synthetic Biology

Expression

Yeast

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge