We narrowed to 11,980 results for: cel.2;

1. LLP733_L2_pV01_0I_FRT-uORF-rLUC_TCTP-fLUC

   Plasmid

   #192376

   Purpose

   To test for the repressive potential of the conserved peptide uORF from AT1G36730 with the FRT sites around it.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-uORF-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LLP727_L2_pV01_0I_TMV-rLUC_TCTP-fLUC

   Plasmid

   #192370

   Purpose

   To control for variation introduced to the gene circuits, Rluc expression not affected by the introduction of terminators or recombination target sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LLP732_L2_pV01_0I_FRT-35Ster-Rluc_lacZ

   Plasmid

   #192375

   Purpose

   To test for the repressive potential of the 35S terminator from CaMV with the FRT sites around it.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-35Ster-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LLP731_L2_pV01_0I_FRT-OCStrunc-Rluc_lacZ

   Plasmid

   #192374

   Purpose

   To test for the repressive potential of the OCS variant (truncation) with the FRT sites around it.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCStrunc-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LLP741_L2pV1_CR_B3RT-Rluc_TCTP-FlpO_lacZ

   Plasmid

   #192385

   Purpose

   To test for if Flp is able to de-repress a circuit with B3RT, to rule out cross-reactivity between these recombinases.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::B3RT-OCS-B3RT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LLP742_L2pV1_CR_FRT-Rluc_TCTP-B3o_lacZ

   Plasmid

   #192386

   Purpose

   To test for if B3 is able to de-repress a circuit with FRT, to rule out cross-reactivity between these recombinases.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LLP757_L2pV3_NOT_B3RT_Rluc_0I_lacZ

   Plasmid

   #192404

   Purpose

   On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   B3RT-Act2-B3RT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LLP756_L2pV1_NOT_B3RT_Rluc_1IB_lacZ

   Plasmid

   #192401

   Purpose

   Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::B3RT-Rluc-B3RT
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LLP755_L2pV1_NOT_B3RT_Rluc_0I_lacZ

   Plasmid

   #192400

   Purpose

   On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::B3RT-Rluc-B3RT
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LLP714_L2pV3_NOT_FRT_Rluc_0I_lacZ

    Plasmid

    #192402

    Purpose

    On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    FRT-Act2-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LLP758_L2pV3_NOT_B3RT_Rluc_1IB_lacZ

    Plasmid

    #192405

    Purpose

    Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    B3RT-Act2-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LLP713_L2pV3_NOT_FRT_Rluc_1IF_lacZ

    Plasmid

    #192403

    Purpose

    Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    FRT-Act2-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LLP738_L2pV1_1I_Act2_FRT_OCS_s35S_Flp_lacZ

    Plasmid

    #192381

    Purpose

    To test if the 35S promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LLP736_L2pV1_1I_Act2_FRT_OCS_TCTP_Flp_lacZ

    Plasmid

    #192379

    Purpose

    To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output (despite the presence of the lacZ endlinker).

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mCerulean3-13aa-Venus-pEGFP-C3

    Plasmid

    #166753

    Purpose

    For expression of mCerulean3 linked to Venus by 13 amino acid linker

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    mCerulean3-13aa-Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mCerulean3-39aa-Venus-pEGFP-C3

    Plasmid

    #166755

    Purpose

    For expression of mCerulean3 linked to Venus by 39 amino acid linker

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    mCerulean3-39aa-Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mCerulean3-31aa-Venus-pEGFP-C3

    Plasmid

    #166754

    Purpose

    For expression of mCerulean3 linked to Venus by 31 amino acid linker

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    mCerulean3-31aa-Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTC366

    Plasmid

    #91215

    Purpose

    protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, ribozyme processing)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    2 gRNAs targeting tomato ARF8A
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWN10042

    Plasmid

    #89052

    Purpose

    GF-ori-FP plasmid for GFxFP assay

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    EGFP part 1
    EGFP part 2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and none

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Banshee-ShCit-A

    Plasmid

    #85216

    Purpose

    Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.

    Depositor

    Alex Minella

    Article

    Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

    Insert

    shCit-A
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge