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pCMV-CIB1-mCerulean-MP

Plasmid

#58366

Purpose

For use in light-induced protein inactivation through the interaction with CRY2

Depositor

Won Do Heo

Article

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Inserts

CIB1[1-147] (CIB1 Mustard Weed)
Camk2a[315-478] (Camk2a Rat)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-EYFP

Plasmid

#231927

Purpose

Cre-off/Flp-on EYFP under the Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV

Promoter

Ef1a

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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DRH334: FCK-QuasAr2-mO2

Plasmid

#51692

Purpose

Fluorescent reporter of membrane voltage with improved sensitivity

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

QuasAr2-mOrange

Use

Lentiviral

Tags

mOrange2

Expression

Mammalian

Promoter

CaMKIIa

Available Since

April 25, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-CoChR-GFP

Plasmid

#59070

Purpose

AAV-mediated expression of CoChR-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

CoChR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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FCK-ChrimsonR-GFP

Plasmid

#59049

Purpose

Lentiviral expression of ChrimsonR under the CaMKII promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-GFP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

K176R

Promoter

CaMKII

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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CD44S pWZL-Blast

Plasmid

#19126

Depositor

Bob Weinberg

Article

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

CD44 standard (CD44 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

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DRH296: FCK-Optopatch2

Plasmid

#51694

Purpose

Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

Optopatch2

Use

Lentiviral

Expression

Mammalian

Promoter

CaMKIIa

Available Since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

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TetO-dCas9-D3A

Plasmid

#78254

Purpose

Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoter

Depositor

Grant Challen

Article

McDonald et al Biol Open. 2016 May 11. pii: bio.019067.

Insert

DNMT3A CD aa 598-912 (DNMT3A Human)

Use

CRISPR

Tags

Flag epitope tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pLX304-ERBB2

Plasmid

#175846

Purpose

Expresses ERBB2

Depositor

Evan Braunstein

Article

Braunstein et al Leukemia. 2016 Nov;30(11):2242-2245. doi

Insert

ERBB2 (ERBB2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Chronos-GFP

Plasmid

#59170

Purpose

AAV-mediated expression of Chronos-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Has Service

AAV Retrograde, AAV1, and AAV5

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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FUW-ubiquitin-EphB3-SV40-GFP (B3G)

Plasmid

#65443

Purpose

Expression of EPHB3 and GFP in mammalian cells

Depositor

Eduard Batlle

Article

Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

Insert

EPHB3 (EPHB3 Human)

Use

Lentiviral

Tags

SV40-EGFP

Expression

Mammalian

Promoter

UBQ

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-vCre

Plasmid

#55638

Purpose

EF1a-driven expression of VCre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde and AAV8

Insert

vCre

Use

AAV

Expression

Mammalian

Promoter

Ef1a

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf6(WT)-HA

Plasmid

#79424

Purpose

Expresses C-terminally HA-tagged Arf6(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf6 (ARF6 Human)

Tags

HA

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn Coff/FonEYFP

Plasmid

#55652

Purpose

Cre-off/Flp-on EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV; Cre off/flp on eyfp

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-EGF-ScNeo

Plasmid

#209893

Purpose

To monitor the status of EGF, the plasmid encodes a recombinant EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

EGF-ScNeo (EGF Human)

Use

Lentiviral

Tags

GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-HBEGF-ScNeo

Plasmid

#209894

Purpose

To monitor the status of HB-EGF, the plasmid encodes a recombinant HB-EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

HBEGF-ScNeo (HBEGF Human)

Use

Lentiviral

Tags

mNeonGreen and mScarlet

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-G-AsCas12a crRNA-BsaI cassette (MSP3491)

Plasmid

#114067

Purpose

T7 promoter crRNA entry vector used for in vitro transcription of AsCas12a guides

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

entry vector for AsCas12a crRNAs

Use

In vitro transcription

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pFB2G

Plasmid

#195290

Purpose

Bicistronic HSV-TK and mTagBFP resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A mTagBFP

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.SF-iGluSnFR.A184S

Plasmid

#106198

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf1(WT)-mCherry

Plasmid

#79419

Purpose

Expresses C-terminally mCherry-tagged Arf1(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf1 (ARF1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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