We narrowed to 285 results for: pspcas9

1. PX458 V3

   Plasmid

   #226957

   Purpose

   CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. SaCas9v2-Puro

   Plasmid

   #178802

   Purpose

   SaCas9 with 2A-Puro, and a cloning backbone for sgRNA.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh (Cas9-2A Puro) and U6 (gRNA)

   Available since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. SOX17-3'gRNA

   Plasmid

   #210465

   Purpose

   gRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-in

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Insert

   SOX17 (SOX17 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX459-CTNNB1-S45

   Plasmid

   #164587

   Purpose

   Expresses a gRNA that overlaps the S45 codon of human CTNNB1

   Depositor

   Renee van Amerongen

   Article

   de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

   Insert

   CTNNB1 gRNA (CTNNB1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Px458-Cas9n-Trp63-Exon4-1sgRNA

   Plasmid

   #88848

   Purpose

   CRISPR KO of Trp63

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp63 (Trp63 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

6. lentiGuideFB-Puro-A

   Plasmid

   #192506

   Purpose

   For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a nd a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

   Insert

   hU6-sgRNA-CS1-BsmBI-EFS-Puro-WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. lentiGuideFB-Puro-B

   Plasmid

   #192507

   Purpose

   For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

   Insert

   bU6-sgRNACR2-CS-BsmBI-EFS-Puro-WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   bU6

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Px458-Cas9n-Trp63-Exon4-2sgRNA

   Plasmid

   #88849

   Purpose

   CRISPR KO of Trp63

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp63 (Trp63 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

9. Px458-Cas9n-Trp73-Exon4-3sgRNA

   Plasmid

   #88850

   Purpose

   CRISPR KO of Trp73

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp73 (Trp73 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

10. Px458-Cas9n-Trp73-Exon4-4sgRNA

    Plasmid

    #88851

    Purpose

    CRISPR KO of Trp73

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp73 (Trp73 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only