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pnEA-NpM-HsNot4_400-424_AG

Plasmid

#148790

Purpose

E. coli of HsNot4_400-424

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_400-424 (CNOT4 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot4_429-575-GB1His6_AG

Plasmid

#148791

Purpose

Bacterial Expression of HsNot4_429-575

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_429-575 (CNOT4 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4_275-376_AG

Plasmid

#148751

Purpose

Mammalian Expression of HsNot4_275-376

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-376 (CNOT4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4_275-575-FFTLtoDAEE_AG

Plasmid

#148756

Purpose

Mammalian Expression of HsNot4_275-575-FFTLtoDAEE

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-FFTLtoDAEE (CNOT4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4_429-575_AG

Plasmid

#148758

Purpose

Mammalian Expression of HsNot4_429-575

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_429-575 (CNOT4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4-del377-424_AG

Plasmid

#148760

Purpose

Mammalian Expression of HsNot4-del377-424

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4-del377-424 (CNOT4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4-FFTLtoDAEE_AG

Plasmid

#148762

Purpose

Mammalian Expression of HsNot4-FFTLtoDAEE

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4-FFTLtoDAEE (CNOT4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4_190-575_AG

Plasmid

#148768

Purpose

Mammalian Expression of HsNot4_190-575

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_190-575 (CNOT4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4_377-428_AG

Plasmid

#148757

Purpose

Mammalian Expression of HsNot4_377-428

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_377-428 (CNOT4 Human)

Expression

Mammalian

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-mut1_G

Plasmid

#146415

Purpose

Mammalian Expression of HsDCP1a-mut1

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1a-mut1 (DCP1A Human)

Expression

Mammalian

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4_275-575_AG

Plasmid

#148752

Purpose

Mammalian Expression of HsNot4_275-575

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575 (CNOT4 Human)

Expression

Mammalian

Available Since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pTMiniTrex-mCherry-Shark3

Plasmid

#233145

Purpose

Serves as a template plasmid for amplifying Shark3 (Theo-OFF) hammerhead aptazyme-3xTy-tagging cassette

Depositor

Ronald Etheridge

Article

Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

Inserts

mCherry
Shark3

Use

Prokaryotic expression

Tags

3x Ty tag

Mutation

Codon optimized for Trypanosoma cruzi and changed…

Available Since

March 20, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pTwist-FLAG-SRSF3_Full.Length_Codon.Optimized

Plasmid

#172345

Purpose

Expression of FLAG-tagged, codon-optimized, full-length SRSF3

Depositor

Sujatha Jagannathan

Article

Campbell et al Cell Rep. 2023 Jun 13;42(6):112642. doi:

Insert

serine and arginine rich splicing factor 3 (SRSF3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized

Promoter

EF-1a

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsEDC4

Plasmid

#66597

Purpose

Expresses lambdaN-HA-tagged EDC4 in mammalian cells

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

EDC4 (EDC4 Human)

Tags

lambdaN-HA

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pDual-eGFP(Y203)

Plasmid

#63559

Purpose

Expression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.

Depositor

Richard S Myers

Article

Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

Insert

His-T7-eGFP(Y203)

Use

Lentiviral

Tags

His6 and T7

Expression

Bacterial and Mammalian

Mutation

Changed threonine at position 203 with respect to…

Promoter

CMV-EF1α hybrid (CEF)

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only
pGAPTrap TALEN 2

Plasmid

#83369

Purpose

Partner to pGAPTrap-TALEN1, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

GAPDH_TALEN2

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only
pTMiniTrex-mCherry-Shark2

Plasmid

#233144

Purpose

Serves as a template plasmid for amplifying Shark2 (Tet-ON) hammerhead aptazyme-3xTy-tagging cassette

Depositor

Ronald Etheridge

Article

Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

Inserts

mCherry
Shark2

Use

Prokaryotic expression

Tags

3x Ty tag

Mutation

Codon optimized for Trypanosoma cruzi

Available Since

March 20, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pGAPTrap-TALEN1

Plasmid

#83368

Purpose

Partner to pGAPTrap-TALEN2, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

GAPDH_TALEN1

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-MS2-HA-HsNot4_AA

Plasmid

#148255

Purpose

Mammalian Expression of HsNot4

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4 (CNOT4 Human)

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Available Since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsDCP1a_G

Plasmid

#146400

Purpose

Mammalian Expression of HsDcp1a

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDcp1a (DCP1A Human)

Expression

Mammalian

Mutation

two silent mutations T237C and G1716T compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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