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pc3XB-ZF91

Plasmid

#13225

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF91

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF94

Plasmid

#13228

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF94

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF97

Plasmid

#13231

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF97

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF99

Plasmid

#13233

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF99

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF101

Plasmid

#13235

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF101

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF104

Plasmid

#13238

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF104

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pc3XB-ZF105

Plasmid

#13239

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF105

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
WT Ecm2

Plasmid

#169922

Purpose

Ecm2 +/-300bp of up- and downstream seq. cloned into the NotI and SalI sites of pRS416

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

Ecm2

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo FlucDM EGFP

Plasmid

#90172

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutation

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Use

Tags

Expression

Mammalian

Mutation

R188Q, R261Q in FLuc

Promoter

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-NR2B

Plasmid

#17925

Depositor

Stefano Vicini

Article

Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

Insert

N-methyl D-aspartate receptor 2B (Grin2b Rat)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
DR274

Plasmid

#42250

Purpose

guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR; Zebrafish expression

Tags

Expression

Worm

Mutation

Promoter

T7

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSC234

Plasmid

#216357

Purpose

xhNup93-Nb3t (+2Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup93-Nb3t (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSC168

Plasmid

#216350

Purpose

xhNup35-Nb1t (+2Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup35-Nb1t (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM6-vioABECD

Plasmid

#66535

Purpose

pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with consensus promoter

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Inserts

VioA
VioB
VioE
VioC
VioD

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Consensus T7

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-NR2A

Plasmid

#17924

Depositor

Stefano Vicini

Article

Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

Insert

NMDAR2A (Grin2a Rat)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-TetO-Sox9

Plasmid

#41080

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

Insert

Sox9 (Sox9 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TetO + minimal CMV

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSC226

Plasmid

#216375

Purpose

xhNup358-Nb2t (+3Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup358-Nb2t (+3Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 puro_shTBK1_3185

Plasmid

#167294

Purpose

Lentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene TBK1. Contains a puro resistance cassette.

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

shRNA TBK1 (TBK1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo FlucSM EGFP

Plasmid

#90171

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutation

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Use

Tags

Expression

Mammalian

Mutation

R188Q in FLuc

Promoter

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDG04439

Plasmid

#216365

Purpose

xhNup133-Nb2t (+2Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup133-Nb2t (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge