user-defined upper limit for the number of target sequences returned
Alignment
region of similarity between target and query sequences
E-value
a BLAST statistic representing the significance of an alignment, values close to zero
indicate high sequence similarity with low probability of the similarity occurring by chance
Identities
the number of exact nucleotide matches over the alignment, expressed as a fraction
and a percentage
Query Coverage
the length of the query sequence that matches the target sequence in the
alignment
Bit Score
a BLAST statistic measuring the quality of an alignment, higher values indicate a
more significant match
Span
the length of the alignment, including gaps
About Search by Sequence
Search by Sequence performs a nucleotide-nucleotide BLAST search against Addgene’s plasmid sequence database.
BLAST returns plasmids with similarity to the query sequence.
Results are sorted by E-value, a statistic from BLAST that describes the significance of a match.
Lower values are considered better matches.
FASTA headers and numbers at the beginning of each line will be removed.
The query should only contain DNA characters.
The minimum query length is 30 nucleotides. Support for short sequence queries is under development.
Tips for Success
Inspect the percent identity, query coverage, and alignment details to determine if a result match is satisfactory.
Visit the corresponding plasmid webpage to view additional details about a matching plasmid.
If no results are returned, try a different isoform or region of the desired sequence. There may not be a match in our database.
You can adjust the Max Results setting on the results page from 25 to 500. If many sequences share the same top E-value,
only a truncated set of equally high-scoring matches will be shown. Set the Max Results to 500 to see more matches.
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An integrating plasmid containing both the inducible enotetSW3 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.
An integrating plasmid containing both the inducible enotetSW1 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.
An integrating plasmid containing both the inducible enotetSW4 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.
A plasmid containing the codon-optimized OsTIR1, which is under the control of adh1 promoter. It served as one of the F-box protein expression plasmids in AID system.
To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with NGN2, Sox11, FGF2 and two small molecules, forskolin and dorsomorphin.
To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with ISL1, LHX3, FGF2 and two small molecules, forskolin and dorsomorphin.
A plasmid containing the ura4 genomic region,which flanked by a NotI site at the both ends. It served as the control plasmid or backbone for the ura4 integrated plasmid construction.