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pAc5.1B-lambdaN-HA-DmUPF1_92-1180-siRNAres-V5His6_N

Plasmid

#146990

Purpose

Insect Expression of DmUPF1_92-1180-siRNAres

Depositor

Elisa Izaurralde

Insert

DmUPF1_92-1180-siRNAres (Upf1 Fly)

Use

Tags

Expression

Insect

Mutation

1 non silent mutations: G995E compared to the seq…

Promoter

Available since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pRDAX-VALENTIA-016 NLSNoLS-Sirius//BsaI

Plasmid

#130197

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

NLSNoLS-Sirius

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-VALENTIA-021 Lamp1-Sirius//BsaI

Plasmid

#130202

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

Lamp1-Sirius

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-VALENTIA-011 Vimentin-Sirius//BsaI

Plasmid

#130192

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

Vimentin-Sirius

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-VALENTIA-006 EB3-Sirius//BsaI

Plasmid

#130187

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

EB3-Sirius

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-VALENTIA-001 LifeAct-Sirius//BsaI

Plasmid

#130182

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

LifeAct-Sirius

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3- SIRT1-NTerm Exon1-Exon2-Exon3

Plasmid

#105680

Purpose

Bacterial Expression construct of N terminus of SIRT1: Exon1-Exon2-Exon3

Depositor

Ullas Kolthur-Seetharam

Article

Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

Insert

SIRT1-NTerm: Exon1-Exon2-Exon3 (Sirt1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Synonymous base changes not affecting the protein…

Promoter

tac_Promoter

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-Cx43-M100/125/147L-siResist

Plasmid

#49856

Purpose

Encodes human connexin 43 with M100, M125, and M147 mutated to L and wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Methionine100, Methionine 125, and Methionine 147…

Promoter

CMV

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only
PB-CuO-V5 CASC3 siRes 1-480 188/218-mut

Plasmid

#158542

Purpose

For PiggyBac-mediated integration of V5-tagged siRNA-resistant EJC-binding deficient CASC3 1-480

Depositor

Niels Gehring

Article

Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

Insert

CASC3; BTZ; MLN51 (CASC3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Amino acids 1-480, C-terminal deletion, F188D, W2…

Promoter

CMV

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-Cx43-M100/125/147/213-siResist

Plasmid

#49858

Purpose

Encodes human connexin 43 with M100, M125, M147, and M213 mutated to L and wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Methionine100, Methionine 125, Methionine 147, an…

Promoter

CMV

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
pDGB3_alpha1_LIR1-35S-eGFP-T35S-SIR-LIR2 (GB4312)

Plasmid

#225435

Purpose

BeYDV LIR1 + TU 35S:eGFP:T35S + BeYDV SIR-LIR2.

Depositor

Diego Orzaez

Insert

LIR1:35S:eGFP:T35S-SIR:LIR2

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-M2WA-CIM1-2mut-siRNAres_S

Plasmid

#147517

Purpose

Insect Expression of HsTNRC6A-M2WA-CIM1-2mut-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A-M2WA-CIM1-2mut-siRNAres (TNRC6A Human)

Use

Tags

Expression

Insect

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-CIM1-2mut-F1359A-siRNAres_R

Plasmid

#147411

Purpose

Insect Expression of HsTNRC6A-CIM1-2mut-F1359A-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A-CIM1-2mut-F1359A-siRNAres (TNRC6A Human)

Use

Tags

Expression

Insect

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A_1-1609-del1384-1524-siRNAres_N

Plasmid

#147024

Purpose

Mammalian Expression of HsTNRC6A_1-1609-del1384-1542-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6A_1-1609-del1384-1542-siRNAres (TNRC6A Human)

Use

Tags

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-del183-258-siRNAres-V5His6_L

Plasmid

#146862

Purpose

Insect Expression of DmPABPC1_del183-258-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmPABPC1_del183-258-siRNAres (pAbp Fly)

Use

Tags

Expression

Insect

Mutation

one non silent mutation G26S, and seven silent mu…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-del288-359-siRNAres-V5His6_L

Plasmid

#146863

Purpose

Insect Expression of DmPABPC1_del288-359-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmPABPC1_del288-359-siRNAres (pAbp Fly)

Use

Tags

Expression

Insect

Mutation

one non silent mutation G26S, and six silent muta…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-del364-550-siRNAres-V5His6_L

Plasmid

#146864

Purpose

Insect Expression of DmPABPC1_del364-550-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmPABPC1_del364-550-siRNAres (pAbp Fly)

Use

Tags

Expression

Insect

Mutation

one non silent mutation G26S, and five silent mut…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-HsSmg6-del42-58-del136-152-siRNAres_K

Plasmid

#146748

Purpose

Mammalian Expression of HsSmg6-del42-58-del136-152-siRNAres

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6-del42-58-del136-152-siRNAres (SMG6 Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations T594C, A699G and two non sil…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pPW3761 : CMVd3-HsIRE1(WLLI-GSGS)[359-362]-HaloTag

Plasmid

#185683

Purpose

Low-level transient expression of IF2 mutant HsIRE1 with a C-terminal HaloTag.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 (ERN1 Synthetic, Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

(WLLI-GSGS)[359-362]

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-M2WA-CIM1-2mut-F1359A-siRNAres_S

Plasmid

#147516

Purpose

Insect Expression of HsTNRC6A-M2WA-CIM1-2mut-F1359A-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A-M2WA-CIM1-2mut-F1359A-siRNAres (TNRC6A Human)

Use

Tags

Expression

Insect

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge