We narrowed to 4,673 results for: pcr
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Plasmid#134436PurposePCR-based C-terminal S6 tagDepositorInsertS6
UsePcr-based tagging in yeastAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOliSpec5'
Plasmid#228551PurposeTemplate for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmidDepositorInsertTruncated 5' region of aadA
UseCRISPRAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOliSpec3'
Plasmid#228550PurposeTemplate for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.DepositorInsertTruncated 3' region of aadA
UseCRISPRAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-His3MX6-PGAL1
Plasmid#41607DepositorPublicationInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…Available sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1
Plasmid#41605DepositorPublicationInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…Available sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFA6a-6xGLY-S-tag-hphMX4
Plasmid#20767DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW/TOPO BMI
Plasmid#69796PurposeGateway entry vector for BMIDepositorInsertBMI (BMI1 Human)
ExpressionBacterialAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL1-pegRNA-attR3
Plasmid#213051PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR3DepositorInsertsgRNA
ExpressionBacterialPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo U6-SpAi9L-U6SpDMDL
Plasmid#78608PurposeExpresses gRNAs (for SpCas9) targeting 5’ of Ai9 stop cassette and Dmd intron 22DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertU6-SpAi9L-U6SpDMDL
UseCRISPRExpressionMammalianPromoterU6Available sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo U6-SpAi9R-U6-SpDMDR
Plasmid#78609PurposeExpresses gRNAs (for SpCas9) targeting 3’ of Ai9 stop cassette and Dmd intron 23DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertU6-SpAi9R-U6-SpDMDR
UseUnspecifiedPromoterU6Available sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-GFP
Plasmid#39289DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…Mutation-1163 to +6 of nmt1 locusAvailable sinceSept. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z21
Plasmid#120064PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 24xSunTagDepositorInsert24xSunTag
UsePcr templateTags24xSunTagPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z03
Plasmid#120046PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxCerulean3DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmoxCerulean3
UsePcr templateTagsmoxCerulean3PromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P10
Plasmid#120021PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKOkDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmKOk
UsePcr templateTagsmKOkPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P01
Plasmid#120012PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxBFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmoxBFP
UsePcr templateTagsmoxBFPPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-28
Plasmid#120007PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with 3xHADepositorInsert3xHA
UsePcr templateTags3xHAPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-17
Plasmid#119996PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with mEos4bDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmEos4b
UsePcr templateTagsmEos4bPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFPcR5
Plasmid#149484PurposeBroad-Host range expression vector, RSF1010 origin, PcaU(AM) repressor, P3B5 promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertPcaU(AM)-mRFP
UseSynthetic BiologyExpressionBacterialPromoterP3B5BAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRIS
Plasmid#120424PurposeThis plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.DepositorInsertCRISPR spacers targeting IS1, IS5, IS3, IS150
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterconstitutiveAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -