We narrowed to 2,464 results for: LARGE

1. Myc-gamma2/deltaILTM4

   Plasmid

   #119735

   Purpose

   GABAA receptor expression (chimeric rat rat gamma 2 subunit whose large intracellular loop, TM4 and C-term have been replaced with the equivalent domains of the rat delta subunit) C-myc near N-term

   Depositor

   Angel de Blas

   Article

   Wu et al J Biol Chem. 2012 Aug 10;287(33):27417-3

   Insert

   GABRG2/GABRD (Gabrg2, Gabrd Rat)
   

   Use

   Tags

   cMyc epitope EQKLISEEDL inserted between fourth a…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PB-TO-hNGN2

   Plasmid

   #172115

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Article

   Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

   Inserts

   hNGN2 (NEUROG2 Human)
   puroR-T2A-mycNLS-mTagBFP2
   

   Use

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and TRE3G

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PB-PG13-mCherry-EF1a-PuroR-EGFP

   Plasmid

   #195292

   Purpose

   piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

   Depositor

   Hideyuki Oguro

   Article

   Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

   Inserts

   mCherry
   PuroR-P2A-EGFP
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PB-TO-hNIL

   Plasmid

   #172113

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Article

   Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

   Inserts

   hNGN2 (NEUROG2 Human)
   hISL1 (ISL1 Human)
   hLHX3 (LHX3 Human)
   puroR-T2A-mycNLS-mTagBFP2
   

   Use

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and TRE3G

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTKA001

   Plasmid

   #164577

   Purpose

   Expresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expression

   Depositor

   Robert Kirchdoerfer

   Article

   Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

   Insert

   nsp7 (ORF1ab )
   

   Use

   Tags

   6xHis, Ek, TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTKA002

   Plasmid

   #164578

   Purpose

   Expresses SARS-CoV-2 nsp8 from a pET46 backbone for IPTG-inducible bacterial expression

   Depositor

   Robert Kirchdoerfer

   Article

   Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

   Insert

   nsp8
   

   Use

   Tags

   6xHis, Ek, TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-SV40LT-IRES-KRABoff-PA-TRE-loxP257-RIR

   Plasmid

   #67277

   Purpose

   inducbile color change and cancer regulation: FlpO recombinase dependent expression of KrasG12D cMyc and SV40 large T antigen with doxycycline inducible downregulation through tetKRAB system.

   Depositor

   Jannik Elverløv-Jakobsen

   Article

   Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do

   Inserts

   first casette contain a red fluorescent protein (katushka) linked to puromycin resitance gene through an E2A site
   second cassette contains mVENUS-kras-cmyc-SV40LT-KRABoff
   

   Use

   Tags

   mTQ2 blue fluorescent and red flourescent gene ka…

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR223-LATS1

   Plasmid

   #23665

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   LATS1 (LATS1 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR223-LATS2

   Plasmid

   #23485

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   LATS2 (LATS2 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   aa182-762 deleted

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pDONR223-DLG3

    Plasmid

    #23451

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    DLG3 (DLG3 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    G381A,G629T

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only