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pAAV.hSynap.(mem).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244098

Purpose

Plasma membrane targeted expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

IgG kappa leader and mIRFP670nano3

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pEMS2393

Plasmid

#246092

Purpose

For preclinical gene therapy: ss-AAV expression of N-term FLAG-tagged human PAX6 under control of Ple331 (PAX6 MiniPromoter); contains WPRE.

Depositor

Elizabeth Simpson

Article

Djaksigulova et al Gene Ther. 2026 Mar 9. doi: 10.1038/s414

Insert

ss-AAV-Ple331-3XFLAG/PAX6-WPRE (PAX6 Human)

Use

AAV

Tags

3XFLAG

Expression

Mammalian

Promoter

Ple311

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-ON-ArgiNLS-AausFP1

Plasmid

#252222

Purpose

Flp-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

AausFP1

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-ON-ArgiNLS-oScarlet

Plasmid

#252221

Purpose

Flp-dependent expression of a single-cell discriminating version of oScarlet fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

oScarlet

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMVp_eGFP

Plasmid

#241002

Purpose

For targeted gene knock-in at NHP IGH locus and constitutive expression of GFP

Depositor

Jennifer Adair

Article

Castelli et al Mol Ther. 2026 Feb 2:S1525-0016(26)00081

Insert

eGFP (eGFP Synthetic)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pVH4p_eGFP

Plasmid

#241003

Purpose

For targeted gene knock-in at NHP IGH locus and B cell-specific expression of GFP

Depositor

Jennifer Adair

Article

Castelli et al Mol Ther. 2026 Feb 2:S1525-0016(26)00081

Insert

eGFP (eGFP )

Use

AAV and CRISPR

Expression

Mammalian

Promoter

VH4

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pOTTC2147 - pAAV nEF ConFon hChR2(H134R)-mCherry

Plasmid

#202545

Purpose

An INTRSECT-ready adeno-associated viral vector expressing channelrhodopsin2-mCherry fusion (Cre-On and Flp-On)

Depositor

Marisela Morales, Christopher Richie

Article

Barbano et al Nat Commun. 2025 Sep 26;16(1):8500. doi:

Insert

hChR2(H134R)-mCherry

Use

AAV

Promoter

nEF

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iGlucoSnFR2.mRuby3

Plasmid

#244071

Purpose

Cytosolic expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

mRuby3

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-mHevin-Myc-His

Plasmid

#249178

Purpose

AAV mediated overexpression of full length mouse Sparcl1

Depositor

Cagla Eroglu

Article

Ramirez et al Neuron. 2026 Feb 19:S0896-6273(25)00984-

Insert

Sparcl1 (Sparcl1 Mouse)

Use

AAV

Tags

6xHis and Myc

Expression

Mammalian

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Gluc-feRMA-IRES-EGFP

Plasmid

#248190

Purpose

Expresses Gluc-feRMA and EGFP under the hSyn promoter. Gluc-feRMA enables monitoring of neuronal transduction and includes a TEV cs, allowing inactivation of it upon interaction with TEV protease.

Depositor

Jerzy Szablowski

Article

Nouraein et al Proc Natl Acad Sci U S A. 2025 Dec 9;122

Inserts

Gaussia luciferase with TEV cleavage site fused to IgG1 Fc
IRES-EGFP

Use

AAV; Luciferase

Tags

Gluc (N terminal on insert) and IgG1 Fc (C termin…

Expression

Mammalian

Promoter

IRES and hSyn

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pFB-EF1a-DIO-Rpl22l1-myc-Flag-2A-tdTomato-WPRE-hGHpA

Plasmid

#246243

Purpose

Cre dependent flag-tagged Rpl22l1.

Depositor

D. James Surmeier

Article

Tanimura et al Neuron. 2019 Feb 6;101(3):444-458.e6. do

Insert

Rpl22l1 (Rpl22l1 Mouse)

Use

AAV

Tags

Myc-FLAG-T2A-tdTomato

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-Pgc-1alpha-EYFP

Plasmid

#241793

Purpose

Expression vector for mouse Pgc-1alpha with EYFP

Depositor

Mary Kay Lobo

Article

Chandra et al Biol Psychiatry. 2017 Apr 1;81(7):564-57

Insert

PGC-1alpha (Ppargc1a Mouse)

Use

AAV

Tags

EYFP

Promoter

Ef1a

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(mem).iGlucoSnFR2.mRuby3

Plasmid

#244082

Purpose

Plasma membrane targeted expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

IgG kappa leader and mRuby3

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-mStayGold

Plasmid

#234549

Purpose

To produce AAV with the P3 promoter to express Alb(mouse)-mStayGold(Ando) fusion protein

Depositor

Hajime Hirase

Article

Knak et al Neurophotonics. 2025 Jun;12(Suppl 2):S22

Insert

Alb-mStayGold

Use

AAV

Mutation

None

Promoter

P3

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-StayGold-P2A-FusionRed

Plasmid

#229563

Purpose

Expresses StayGold and FusionRed in the cytoplasm of mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

StayGold

Use

AAV

Expression

Mammalian

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(ER).iGlucoSnFR2.HaloTag

Plasmid

#244076

Purpose

ER targeted expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Use

AAV

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapFLEX.(cyto).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244100

Purpose

Cytosolic expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

mIRFP670nano3

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).iGlucoSnFR2.H348H.HaloTag

Plasmid

#244086

Purpose

Cytosolic expression of green glucose sensor (high affinity) with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.H348H.HaloTag

Use

AAV

Tags

HaloTag

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244087

Purpose

Cytosolic expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

mIRFP670nano3

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(cyto).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244080

Purpose

Cytosolic expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

mIRFP670nano3

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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