We narrowed to 43,407 results for: cha;
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Plasmid#214410PurposeLipid droplet localization of 6xHp of Spastin fused to mAppleDepositorInsertSpastin aa 43-92 (SPAST Human)
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSG5-HA-ERR3 S45A
Plasmid#37849DepositorInsertEstrogen-related receptor gamma (Esrrg Mouse)
UseTagsHAExpressionMammalianMutationchanged Serine 45 to Alanine (AGC to GCC)PromoterSV40Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChRger3-TS-EYFP
Plasmid#127240PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.DepositorInsertChRger3-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianMutationPromoterhSyn1Available sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
N-BLa 1.2
Plasmid#88999PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.DepositorInsertN-BLa 1.2 fusion protein
UseTagsFlagExpressionBacterialMutationPromoteraraBADAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
C-BLa 1.2
Plasmid#89000PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the C-terminal fragment of the split β-lactamase.DepositorInsertC-BLa 1.2 fusion protein
UseTagsFlagExpressionBacterialMutationPromoteraraBADAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCas9-HE-Geminin
Plasmid#109402PurposeExpresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein and Geminin degronDepositorInsertHE-Geminin
UseTagsExpressionMutationPromoterAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRG/III-hs-MBP-CB2-HF
Plasmid#121682PurposeBacterial expression of cannabinoid receptor CB2 fusion proteinDepositorInsertperipheral cannabinoid receptor (CNR2 Human)
UseTagsHis6 Flag tagExpressionBacterialMutationPromoterlacAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907)
Plasmid#101299PurposeFRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.DepositorInsertalpha-Catenin
UseTagsExpressionMammalianMutationContains amino acids 1-698 and 698-906 of alpha-C…PromoterAvailable sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMax_mOrange_A215C
Plasmid#177828PurposeSite-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).DepositorInsertmOrange_A215C
UseTagsExpressionMammalianMutationA215CPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28-Strep-OPA1(253-960) (SB263)
Plasmid#227612PurposeInducible expression of Strep-tagged Opa1(253-960) in E.coliDepositorInsertOPA1 (OPA1 Human)
UseTagsTwin-StrepTagExpressionBacterialMutationcontains aa 253-960 onlyPromoterT7Available sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
SNX10-PX (1-201)
Plasmid#119091PurposeBacterial expression of human phox homology (PX) domain, SNX10-PX (1-201)DepositorInsertSNX10-PX (1-201) (SNX10 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-FAB(ER-LD)
Plasmid#214420PurposeDetection of ER-lipid droplet contact siteDepositorInsertRspAN-FRB-V5-ER_IRES_CFAST-20nm-Halo-6xHp
UseLentiviralTagsCFAST-Halo and RspAN-FRB-V5ExpressionMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Sc++
Plasmid#155011PurposeExpresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cellsDepositorInsertSc++
UseTagsExpressionMammalianMutationT1227K, Loop Substitution from S. anginosusPromoterEF1ɑAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-eGFP-beta 2 CP
Plasmid#13298DepositorInsertcapping protein beta 2 subunit (Capzb Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMax_GFP_C289T
Plasmid#177826PurposeSite-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).DepositorInsertGFP_C289T
UseTagsExpressionMammalianMutationC289TPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-mMaple3-V157I
Plasmid#98577Purposephotoconvertible fluorescent protein mMaple3-V157I in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coliDepositorInsertmMaple3-V157I
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28-Strep-OPA1(MGD) (SB264)
Plasmid#227613PurposeInducible expression of Strep-tagged Opa1(MGD) in E.coliDepositorInsertOPA1 (OPA1 Human)
UseTagsTwin-StrepTagExpressionBacterialMutationcontains aa 253-580 (MGD) and aa 938-960PromoterT7Available sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSP64-hERG1a
Plasmid#53051PurposeExpresses alpha subunit of hERG1a K channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
UseXenopus oocyte expressionTagsExpressionMutationPromoterSP6Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TRAIL-R2
Plasmid#61383PurposeExpresses TRAIL receptor 2DepositorInsertTRAIL receptor 2 (TNFRSF10B Human)
UseTagsnoneExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
SNX29-PX (269-391)
Plasmid#119108PurposeBacterial expression of human phox homology (PX) domain, SNX29-PX (269-391)DepositorInsertSNX29-PX (269-391)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)
Plasmid#101297PurposeFRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.DepositorInsertalpha-Catenin
UseTagsExpressionMammalianMutationContains amino acids 1-906 of alpha-CateninPromoterAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TEV-TBP6.9F34MF37MF77M
Plasmid#168268PurposeExpresses human TBP6.9 with the F34M/F37M/F77M triple mutantDepositorInsertTBP6.9
UseTags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F34M, F37M and F77MPromoterT7 promotorAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEC3111 (pSpy1C-Plac(3)_ffluc)
Plasmid#218778Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseTagsExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(3)Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
FAB(PX-LD)
Plasmid#214426PurposeDetection of peroxisome-lipid droplet contact siteDepositorInsertFKBP-V5
UseTagsCFAST-Halo and FKBP-V5-RspANExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
mouse Drp1(K38A)
Plasmid#26049DepositorInsertDrp1 (Dnm1l Mouse)
UseTagsExpressionMammalianMutationK38A GTPase mutantPromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pExodus CMV.Trex2
Plasmid#40210PurposeMammalian expression of codon-optimized Trex2.DepositorInsertThree prime repair exonuclease 2 (Trex2 Mouse)
UseTagsExpressionBacterial and MammalianMutationPromoterCMV and T7Available sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMax_BFP_A191G
Plasmid#177823PurposeSite-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).DepositorInserttagBFP_A191G
UseTagsExpressionMammalianMutationA191GPromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChRger1-TS-EYFP
Plasmid#127243PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.DepositorInsertChRger1-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianMutationPromoterhSyn1Available sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pclbw-eGFPomp25
Plasmid#58424PurposeMammalian expression of GFP targeted to the mitochondrial outer membraneDepositorInserteGFP-omp25 (Synj2bp Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationcontains aa's 109-145 of mouse omp25PromoterCMV/b-actinAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-IRAlus-Lin28
Plasmid#92347PurposePlasmid for expression of GFP gene with a Lin28 gene derived IR Alu element containing 3'UTR.DepositorInsertLin28 gene derived IR Alu element containing 3'UTR (LIN28A Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCry2PHR-mCh-RhoA
Plasmid#42958PurposeExpresses optogenetically controlled RhoADepositorInsertCry2PHR-mCherry-RhoA (RHOA Mustard Weed, Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-mKate2.5f
Plasmid#99129PurposeAlso known as pAAV-GFAP-mKate2.5-f. An AAV genome that express the farnesylated fluorescent protein mKate2.5 from the GFAP promoterDepositorInsertmKate2.5f
UseAAVTagsExpressionMammalianMutationPromoterGFAPAvailable sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-MBP-4xDQNAT
Plasmid#128398PurposePeriplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coliDepositorInsertMaltose binding protein
UseTags4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…ExpressionBacterialMutationY342H- please see depositor commentsPromotertrcAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Alu-Lin28
Plasmid#92346PurposePlasmid for expression of GFP gene with a Lin28 gene derived Alu element containing 3'UTR.DepositorInsertLin28 gene derived Alu element containing 3'UTR (LIN28A Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 E-cadherin shRNA/Ecad-tdTomato
Plasmid#101278PurposeLentiviral expression of shRNA targeting Ecad and Ecad-tdtomatoDepositorInsertE-cadherin
UseLentiviralTagstdtomatoExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA pcDNA3
Plasmid#206051Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain IIDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRR-ERbeta-5Z
Plasmid#23062DepositorInsertERbeta (ESR2 Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pQFmcs-2H12.D11-scarREF
Plasmid#221184PurposecdGreen2 expressed from a cumate-inducible promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11240)DepositorInsertscdGreen2
mScarlet-I
UseTagsExpressionBacterialMutationN-terminus changed: starts with MSKKYGEAVIKE ...PromoterNone (same as cdGreen2) and PQ5 (cumate-inducible)Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-VdC9BV
Plasmid#62195PurposeExpresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous GenesDepositorInsertVP64dCas9BFPVP64
UseLentiviralTagsTwo VP64s tagged to dCas9 fused to BFPExpressionMammalianMutationD10A H840A (catalytically inactive) Cas9 (dCas9)PromoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-FHC-POLQ-DM
Plasmid#64880Purposelentiviral expression of human POLQ double mutant (in POL and HDL domains)DepositorInsertPOLQ (POLQ Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationK121M and D2330A,Y2331APromoterEF1alphaAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only