We narrowed to 22,255 results for: SCR

1. pRS_1N_400

   Plasmid

   #170476

   Purpose

   To amplify transcription template containing LacI operators

   Depositor

   Laura Finzi

   Article

   Xu et al Nucleic Acids Res. 2022 Mar 21;50(5):282

   Insert

   T7A1, O1, O-, Lambda t1 in lacZ fragment
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7A1

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pELF4.1.0-gDNA

   Plasmid

   #112467

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ELF4

   Depositor

   Kevin White

   Insert

   ELF4 (ELF4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pE2F5.1.0-gDNA

   Plasmid

   #112447

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor E2F5

   Depositor

   Kevin White

   Insert

   E2F5 (E2F5 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHOXC13-donor

   Plasmid

   #113827

   Purpose

   CRISPR donor plasmid to tag human transcription factor HOXC13 with GFP

   Depositor

   Kevin White

   Insert

   HOXC13 homology arms flanking EGFP-IRES-Neo cassette (HOXC13 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGLIS3-donor

   Plasmid

   #113812

   Purpose

   CRISPR donor plasmid to tag human transcription factor GLIS3 with GFP

   Depositor

   Kevin White

   Insert

   GLIS3 homology arms flanking EGFP-IRES-Neo cassette (GLIS3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   rs10118031, rs10973702, rs912257

   Promoter

   Available since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSRF-donor

   Plasmid

   #112362

   Purpose

   CRISPR donor plasmid to tag human transcription factor SRF with GFP

   Depositor

   Kevin White

   Insert

   SRF homology arms flanking EGFP-IRES-Neo cassette (SRF Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   hg38:6:43178470:C>A, hg38:6:43178514:G>A

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHBP1-donor

   Plasmid

   #112336

   Purpose

   CRISPR donor plasmid to tag human transcription factor HBP1 with GFP

   Depositor

   Kevin White

   Insert

   HBP1 homology arms flanking EGFP-IRES-Neo cassette (HBP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZNF785-donor

   Plasmid

   #112317

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF785 with GFP

   Depositor

   Kevin White

   Insert

   ZNF785 homology arms flanking EGFP-IRES-Neo cassette (ZNF785 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   hg38:16:30582292:A>T, hg38:16:30582302:A>T,…

   Promoter

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZNF354B-donor

   Plasmid

   #112309

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF354B with GFP

   Depositor

   Kevin White

   Insert

   ZNF354B homology arms flanking EGFP-IRES-Neo cassette (ZNF354B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   hg38:5:178884427:G>A, hg38:5:178883445:C>T,…

   Promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTOPO-rDNA-NTS

    Plasmid

    #23992

    Depositor

    Susan Baserga

    Article

    Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

    Insert

    rDNA NTS
    

    Use

    Bacterial cloning - topo

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pDD2045

    Plasmid

    #101379

    Purpose

    Name: pBluescript-empty vector. pBluescript-LANApi,K14 (pDD2000) bidirectional empty vector for backbone only.

    Depositor

    Dirk Dittmer

    Article

    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI152-pLenti-SiT-Cas12a

    Plasmid

    #128405

    Purpose

    Express both AsCas12a and CRISPR arrays on single transcripts

    Depositor

    Randall Platt

    Article

    Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

    Insert

    AsCas12a-Triplex
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-6xHis-MBP-TEV site-TadA8.20

    Plasmid

    #194702

    Purpose

    Expresses N-terminal MBP fused TadA8.20 in bacteria

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    TadA8.20
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    mutations in TadA are described in the paper

    Promoter

    T7 promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tet-inducible Luciferase reporter

    Plasmid

    #64127

    Purpose

    Photoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetOx13-CMV

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

    Plasmid

    #225756

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTPGI_dCas9_VP64

    Plasmid

    #49013

    Purpose

    encodes yeast-optimized dCas9_VP64 synthetic transcription factor

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    pTPGI (galactose+aTc inducible)

    Available since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. GAL4UAS-Luciferase reporter

    Plasmid

    #64125

    Purpose

    Photoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    GAL4UAS
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    GAL4UAS

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX856

    Plasmid

    #62888

    Purpose

    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa536-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS, NLS SV40, and VP64

    Expression

    Mammalian

    Mutation

    Asparagine 863 to Alanine (N863A)

    Promoter

    CBh

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX855

    Plasmid

    #62887

    Purpose

    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Aspartic acide 10 to Alanine (D10A)

    Promoter

    CBh

    Available since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pG12-SEAP

    Plasmid

    #170271

    Purpose

    Secreted alkaline phosphatase (SEAP) expression in mammalian cells. Reporter plasmid.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    SEAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    12xUAS with TATA-box minimal promoter

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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