We narrowed to 23,619 results for: CHI

1. pEYmRD3A

   Plasmid

   #127756

   Purpose

   Research the effect of gene orientation of transcription

   Depositor

   Richard Murray

   Article

   Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

   Insert

   mSpinach,RFP Divergent Orientation
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. mNG2(1-10)_tdTomato

   Plasmid

   #184169

   Purpose

   Lentiviral vector for expression and selection of the mNG2(1-10) split fluorescent protein.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Inserts

   mNG2(1-10)
   tdTomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpyCatcher003

   Plasmid

   #133447

   Purpose

   Expresses SpyCatcher003 in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyCatcher003
   

   Use

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJH2930

   Plasmid

   #175283

   Purpose

   Pges-1 DAF-16a unc-54 3' UTR C.elegans gut expression of isoform a of DAF-16

   Depositor

   Mei Zhen

   Article

   Hung et al Development. 2014 Apr;141(8):1767-79. do

   Insert

   DAF-16a (daf-16 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Pges-1

   Available since

   Dec. 3, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pTRKH3-ermGFP

   Plasmid

   #27169

   Depositor

   Michela Lizier

   Article

   Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

   Insert

   erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   presence of an additional 183-bp region at N term…

   Promoter

   Available since

   Feb. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. p4455 FLAG-hPLIC-2

   Plasmid

   #8661

   Depositor

   Peter Howley

   Article

   Kleijnen et al Mol Cell 2000 Aug;6(2):409-19.

   Insert

   hPLIC-2 (UBQLN2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-Aurora A

   Plasmid

   #198402

   Purpose

   Expression of EGFP-tagged Aurora A in mammalian cells

   Depositor

   Kenji Sugimoto

   Article

   Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

   Insert

   Aurora A
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTW1169

   Plasmid

   #236583

   Purpose

   TRV1

   Depositor

   Steven Jacobsen

   Article

   Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

   Insert

   TRV1
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX6P1-GFP-Nanobody

   Plasmid

   #61838

   Purpose

   Expresses GST-tagged anti-GFP Nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

   Insert

   GFP-Nanobody
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pComb3XSS

    Plasmid

    #63890

    Purpose

    3rd generation plasmid for phage display on modified geneIII, contains stuffer fragment

    Depositor

    Carlos Barbas

    Article

    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

    Inserts

    SS Stuffer
    Light Chain Stuffer
    Heavy Chain Stuffer
    

    Use

    Phage display

    Tags

    6x His, HA tag, and geneIII

    Expression

    Bacterial

    Mutation

    Promoter

    lacZ

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hsyn-NE2m

    Plasmid

    #208686

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m in neurons

    Depositor

    Yulong Li

    Insert

    GPCR activation based norepinephrine (NE) sensor GRAB_NE2m
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEC3135 (pERASE)

    Plasmid

    #220306

    Purpose

    Enables scarless gene deletions in Streptococcus pyogenes using the counterselection marker pheS*

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBTK622

    Plasmid

    #180592

    Purpose

    tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1

    Depositor

    Jeffrey Barrick

    Article

    Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585

    Inserts

    tdk
    kanR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC19-TgKI-MCS-eGFP_Y40N-MCS-polyA

    Plasmid

    #174025

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    eGFP
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Y40N

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJCX4

    Plasmid

    #170756

    Purpose

    Express in mammalian cells a target protein C-terminally fused to the Mxe GryA intein - chitin binding domain (CBD) tag.

    Depositor

    Roberto Dominguez

    Article

    Carman et al J Biol Chem. 2021 Oct;297(4):101154. doi

    Type

    Empty backbone

    Use

    Tags

    Mxe GyrA intein - chitin binding domain (CBD)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLsCas13a-gRNA-NT

    Plasmid

    #164857

    Purpose

    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    LsCas13a-gRNA-NT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GLUT1-eGFP/pcDNA-DEST47

    Plasmid

    #18729

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    pcDNA-DEST47 /hGLUT1-eGFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted TAA

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mito-mScarlet

    Plasmid

    #141153

    Purpose

    Fluorescent labeling of the mitochondrial matrix in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    COX4 (COX4 Budding Yeast)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFA6a-kanMX6

    Plasmid

    #39296

    Depositor

    John Pringle, Jurg Bahler

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGateway 3XFlag Prickle1 flag tagged

    Plasmid

    #24644

    Depositor

    Jeff Wrana

    Article

    Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

    Insert

    Prickle 1 (PRICKLE1 Human)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

21. pGateway 3XFlag Prickle2 flag tagged

    Plasmid

    #24645

    Depositor

    Jeff Wrana

    Article

    Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

    Insert

    Prickle 2 (PRICKLE2 Human)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

22. pUC19-TgKI-MCS-p2A-mScarlet-MCS-polyA

    Plasmid

    #174029

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    p2A-mScarlet
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV4-Tax WT

    Plasmid

    #23284

    Depositor

    Warner Greene

    Article

    Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

    Insert

    Tax
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. SpyCatcher003-sfGFP

    Plasmid

    #133449

    Purpose

    Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyCatcher003-sfGFP
    

    Use

    Tags

    His6 and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-MBP-mSA2

    Plasmid

    #52319

    Purpose

    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

    Depositor

    Sheldon Park

    Article

    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

    Insert

    monomeric streptavidin 2
    

    Use

    Tags

    FLAG, His, MBP, and TEV

    Expression

    Bacterial

    Mutation

    S25H mutation compared to streptavidin

    Promoter

    T7

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPP441

    Plasmid

    #158801

    Purpose

    Plasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-2
    

    Use

    Tags

    2xSV40NLS-2xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mPiezo2-CMV-Sport6

    Plasmid

    #81073

    Purpose

    mouse Piezo2

    Depositor

    Ardem Patapoutian

    Article

    Coste et al Nat Commun. 2015 May 26;6:7223. doi: 10.

    Insert

    Piezo2 (please see depositor comments below) (Piezo2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDV002

    Plasmid

    #191823

    Purpose

    Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the NS1 locus. No promoter. No terminator. Golden Gate compatible. Selection: in A. platensis: aadA; E. coli: AmpR

    Depositor

    Jim Roberts

    Article

    Tabakh et al STAR Protoc. 2023 Feb 8;4(1):102087. doi

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSR58.6

    Plasmid

    #63176

    Purpose

    Expresses CcaR constitutively and sfGFP under the PcpcG2-172 promoter

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    CcaR
    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSR43.6r

    Plasmid

    #63197

    Purpose

    Expresses CcaS, Ho1, and PcyA constitutively

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    CcaS
    Ho1
    PcyA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge