We narrowed to 5,822 results for: pCas

1. GB4401

   Plasmid

   #215252

   Purpose

   Module for the expression of the N. nambi HISPS gene under the regulation of PNOS and TNOS and the constitutive expression of CPH, LUZ, H3H, EGFP and p19.

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   SF-PNOS:HISPS:TNOS-CPH-LUZ-H3H-EGFP-p19
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC57-Amp_CRISPIE donor B2

   Plasmid

   #172843

   Purpose

   CRISPIE donor B2 (Zhong et al, eLife 2021), mRuby3 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 or DRS-2 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   CRISPIE designer exon (phase 0-0) encoding mRuby3
   

   Use

   CRISPR

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pREDIT_Cas9-MS2-BB_BbsI

   Plasmid

   #164802

   Purpose

   REDIT backbone for wildtype Cas9, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9-T2A-EBFP

   Depositor

   Le Cong

   Article

   Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. T/brachyury(2A-Dre)

   Plasmid

   #140761

   Purpose

   Targeting vector to T/brachyury gene locus for induction of P2A-fused Dre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F3) sequence.

   Depositor

   Hideyuki Okano

   Article

   Serizawa et al Development. 2019 Nov 4;146(21). pii: de

   Insert

   Dre
   

   Use

   Mouse Targeting

   Promoter

   Endogenous T/brachyury

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXEE401B

   Plasmid

   #91716

   Purpose

   CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Bar resistance

   Depositor

   Qi-Jun Chen

   Article

   Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV8-cPE-C

   Plasmid

   #183539

   Purpose

   Deliver a split compact Prime Editor in vivo

   Depositor

   Wen Xue

   Article

   Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

   Insert

   C-terminal fragment of SpCas9 nickase (H840A), Reverse Transcriptase
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U1A

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAdEasy Flag GCN5

   Plasmid

   #14106

   Depositor

   Pere Puigserver

   Article

   Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

   Insert

   GCN5 (KAT2A Human)
   

   Use

   Adenoviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   E189G and K195N

   Available Since

   Feb. 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS315e_pGal-nCas9(D10A)-PmCDA1

   Plasmid

   #79617

   Purpose

   Expresses nCas9(D10A)-PmCDA1 in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Tags

   PmCDA1

   Expression

   Yeast

   Mutation

   D10A for nickase Cas9

   Promoter

   pGal1

   Available Since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pREDIT_Cas9n-MS2-BB_BbsI

   Plasmid

   #164804

   Purpose

   REDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFP

   Depositor

   Le Cong

   Article

   Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. ABE8e-dimer

    Plasmid

    #138490

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-nSpCas9
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pROF417

    Plasmid

    #155335

    Purpose

    Constitutive expression LFY-VP16-2A-RLuc in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-LFY-VP16-NLS-2A-RLuc-Tnos
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLC133

    Plasmid

    #198639

    Purpose

    Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

    Depositor

    David Bikard

    Article

    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

    Insert

    dCas9
    

    Tags

    3x-FLAG

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMH-Cas9-gate

    Plasmid

    #113742

    Purpose

    Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving hygromycin for selection in plants.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    Cas9
    

    Use

    CRISPR; Gateway destination vector

    Promoter

    Z. Mays ubiquitin promoter, 35S promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPPEM

    Plasmid

    #162467

    Purpose

    Destination Expression vector for monocot plant primer editing

    Depositor

    Adam Bogdanove

    Article

    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

    Insert

    Cas9H840A
    

    Tags

    MLV reverse transcriptase

    Expression

    Plant

    Mutation

    Change histidine 840 to Alanine

    Promoter

    maize Ubi

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. TDP43 NOTAG2

    Plasmid

    #28207

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    aa86-414 only

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

16. AAV8-cPE-N

    Plasmid

    #183538

    Purpose

    Deliver a split compact Prime Editor in vivo

    Depositor

    Wen Xue

    Article

    Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

    Insert

    N-terminal fragment of SpCas9 nickase (H840A)
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCLV3Cas-LT1

    Plasmid

    #210549

    Purpose

    Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT1::mCherrySSM
    

    Expression

    Plant

    Mutation

    2bp insertion after AAVS1 target sequence that di…

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS315e_pGal-dCas9-PmCDA1

    Plasmid

    #79615

    Purpose

    Expresses dCas9-PmCDA1 in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Tags

    PmCDA1

    Expression

    Yeast

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pGal1

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1_Cas9-hGem_gRNA2

    Plasmid

    #217659

    Purpose

    expresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locus

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    sgRNA2 targeting the human AAVS1 safe harbour locus
    

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    U6

    Available Since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. PX459-sgRNA048_Grb10

    Plasmid

    #232934

    Purpose

    Expression vector for a sgRNA against the mouse Grb10 locus and SpCas9 with T2A-Puromycin resistance gene.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    Cas9-T2A-PuroR (Grb10 S. pyogenes)
    

    Expression

    Mammalian

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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