Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 5,537 results for: PID

Showing: 4861 - 4880 of 5537 results

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pAG416GAL10-POWV_NS2B-GS-NS3

Plasmid

#203471

Purpose

Expresses POWV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

POWV NS2B-GS-NS3 (POLY )

Expression

Yeast

Promoter

GAL10

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-LGTV_NS2B-GS-NS3

Plasmid

#203468

Purpose

Expresses LGTV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

LGTV NS2B-GS-NS3 (POLY )

Expression

Yeast

Promoter

GAL10

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-YES1-6stop

Plasmid

#203476

Purpose

Expresses human YES1 kinase from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

YES1 (YES1 Human)

Expression

Yeast

Promoter

GAL10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-SARS-CoV_PLP

Plasmid

#201942

Purpose

Expresses SARS-CoV papain-like protease from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

SARS-CoV PLP (ORF1ab )

Expression

Yeast

Promoter

GAL1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GALL-SARS-CoV-2_3CL

Plasmid

#203496

Purpose

Expresses SARS-CoV-2 3CL protease from a GALL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

SARS-CoV-2 3CL protease (ORF1ab )

Expression

Yeast

Promoter

GALL

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-MERS-CoV_PLP

Plasmid

#203508

Purpose

Expresses MERS-CoV papain-like protease from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

MERS-CoV PLP (orf1ab )

Expression

Yeast

Promoter

GAL1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-CCHFV_OTU

Plasmid

#201945

Purpose

Expresses CCHFV OTU domain of L protein from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

CCHFV OTU domain of L protein (RdRp )

Expression

Yeast

Promoter

GAL10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GALL-SARS-CoV_3CL

Plasmid

#203495

Purpose

Expresses SARS-CoV 3CL protease from a GALL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

SARS-CoV 3CL protease (ORF1ab )

Expression

Yeast

Promoter

GALL

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-2x-uA-BWCoV-SW1_3CL

Plasmid

#201936

Purpose

Expresses Beluga Whale-CoV SW1 3CL protease from a GAL-2x promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

Beluga Whale-CoV SW1 3CL protease

Expression

Yeast

Promoter

GAL1

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-DUGV_OTU

Plasmid

#203463

Purpose

Expresses DUGV OTU domain of L protein from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

DUGV OTU domain of L protein (DUGVsLgp1 )

Expression

Yeast

Promoter

GAL10

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-3x-uC-HCoV-NL63_3CL

Plasmid

#201938

Purpose

Expresses HCoV-NL63 3CL protease from a GAL-3x promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HCoV-NL63 3CL protease (ORF1ab )

Expression

Yeast

Promoter

GAL1

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-HCoV-NL63_PLP2

Plasmid

#203502

Purpose

Expresses HCoV-NL63 PLP2 from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HCoV-NL63 PLP2 (ORF1ab )

Expression

Yeast

Promoter

GAL1

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-ZIKV_NS2B-GS-NS3

Plasmid

#203477

Purpose

Expresses ZIKV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

ZIKV NS2B-GS-NS3 (POLY )

Expression

Yeast

Promoter

GAL10

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-HCoV-OC43_3CL

Plasmid

#203503

Purpose

Expresses HCoV-OC43 3CL protease from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HCoV-OC43 3CL protease (ORF1ab )

Expression

Yeast

Promoter

GAL1

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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mKate2-DrCavin1a in pT3TS-Dest

Plasmid

#194294

Purpose

In vitro transcription of mKate2 tagged zebrafish cavin1a from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAI

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

cavin1a (cavin1a Zebrafish)

Use

In vitro transcription of mrna

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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p3E-HsNRF2 (NFE2L2)

Plasmid

#194302

Purpose

Multisite gateway entry clone for expression of human NRF2 (NFE2L2) with fusion tag at the N-terminus. Parton lab clone KRS

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

NFE2L2 (NFE2L2 Human)

Use

Multisite gateway entry vector

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET15b MOSPD2 [315-445]

Plasmid

#186474

Purpose

Expression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteria

Depositor

Catherine Tomasetto

Article

Di Mattia et al EMBO J. 2020 Dec 1;39(23):e104369. doi:

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Tags

6HIS

Expression

Bacterial

Mutation

MSP domain [315-445]

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pPICZ-His6-K2P4.1A-mCherry-StreptagII

Plasmid

#158743

Purpose

Expresses K2P4.1 isoform A with TEV protease cleavable N- and C-terminal affinity tags.

Depositor

Arthur Laganowsky

Article

Schrecke et al Nat Chem Biol. 2021 Jan;17(1):89-95. doi

Insert

K2P4.1-A (KCNK4 Human)

Tags

His6 and mCherry with Streptag II

Expression

Yeast

Mutation

Residues 1-264 with N-linked glycosylation sites …

Promoter

AOX1

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pPICZ-His6-K2P4.1B-mCherry-StreptagII

Plasmid

#158744

Purpose

Expresses K2P4.1 isoform B with TEV protease cleavable N- and C-terminal affinity tags.

Depositor

Arthur Laganowsky

Article

Schrecke et al Nat Chem Biol. 2021 Jan;17(1):89-95. doi

Insert

K2P4.1-B (KCNK4 Human)

Tags

His6 and mCherry with Streptag II

Expression

Yeast

Mutation

Residues 1-290 and N-linked glycosylation sites r…

Promoter

AOX1

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_mCherry_Luciferase_sgRNA

Plasmid

#155109

Purpose

lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting Luciferase

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

Luciferase_sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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