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pNL1 Ecarin

Plasmid

#106289

Purpose

Expresses Ecarin in Ecoli off a T7 promoter in the NEB control cloning vector

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

Ecarin

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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907K

Plasmid

#160236

Purpose

FlightN-PipR-VP16-Sv40-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

pristinamycin repressor
Flightin promoter

Tags

VP16

Expression

Insect

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSO7

Plasmid

#106365

Purpose

To insert a single copy of a construct at a site on Chr I, corresponding to the Shigella flexneri protein OspF (a MAP kinase inhibitor) expressed specifically in the epidermis.

Depositor

Jonathan Ewbank

Article

Lee et al Virulence. 2018 Dec 31;9(1):648-658. doi

Insert

ttTi4348_ SEC _pcol-19_OspF_3'unc54UTR

Expression

Worm

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-GAP1IP4BPdeltaC2

Plasmid

#20199

Depositor

Robin Irvine

Article

Hammond et al J Cell Biol. 2009 Jan 26;184(2):297-308.

Insert

GAP1IP4BP-detlaC2 (RASA3 Human)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of C2 domains

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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Oncostatin M toehold switch sensor

Plasmid

#111909

Purpose

Toehold switch sensor to detect Oncostatin M mRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

Oncostatin M toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pAP1680

Plasmid

#137088

Purpose

Plasmid for PCR-generated donor DNA for rapid tagging of proteins with tagRFP

Depositor

Geraldine Seydoux

Article

Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

Insert

tagRFP

Use

Pcr template for donor dna

Expression

Bacterial

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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Cav1-miniSOG

Plasmid

#67667

Purpose

Mammalian expression vector driving dog Caveolin1-minSOG

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

Dog Cav1-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 Y292C

Plasmid

#87291

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 Y292C

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

Y292C

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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CBP-Bromo-HAT-SP65

Plasmid

#21090

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Use

In vitro tnt

Tags

Flag

Mutation

Bromo+HAT domains aa 1107-1621

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pMCh-56

Plasmid

#92260

Purpose

Plasmid for expression of HA-tagged Drosophila melanogaster GW182 in D. melanogaster cells

Depositor

Marina Chekulaeva, Roy Parker

Article

Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

Insert

dGW182 (gw Fly)

Tags

HA

Expression

Insect

Promoter

Ac5

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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Pr-delRBS-deGFP

Plasmid

#67744

Purpose

Pr promoter expressing a deGFP that is missing a ribosomal binding site

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

deGFP-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pMT mir-1-1 reporter cel-mir-40

Plasmid

#46739

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-40

Use

RNAi

Tags

His and hsa-mir-1-1

Expression

Insect

Promoter

Metallothionein (MT)

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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MAGE-A2Pro pGL4basic

Plasmid

#78137

Purpose

luciferase reporter plasmid

Depositor

Heidi Schwarzenbach

Article

Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

Insert

MAGE-A2 (MAGEA2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

contains -549/+332 of promoter

Promoter

MAGE-A2

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only
MAGE-A1Pro pGL4basic

Plasmid

#78136

Purpose

luciferase reporter plasmid

Depositor

Heidi Schwarzenbach

Article

Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

Insert

MAGE-A1 (MAGEA1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

contains -82/+116 of promoter

Promoter

MAGE-A1

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pDD401

Plasmid

#91828

Purpose

Pmyo-2::GFP FP-slot donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Pmyo-2::GFP

Use

CRISPR

Expression

Worm

Mutation

Nucleotide 489 of the myo-2 promoter was changed …

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-R1ACR_653-EYFP

Plasmid

#111079

Purpose

Anion channelrhodopsin from Rhodomonas sp. for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin R1ACR_653

Tags

EYFP

Expression

Mammalian

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pNP1 AND gate sfGFP

Plasmid

#107361

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 AND input A and pNP1 AND input B are co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

AND gate + sfGFP

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pattB-3xFlag-Loqs-PB

Plasmid

#41109

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

Loquacious-PB (loqs Fly)

Tags

3xFlag

Expression

Insect

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
PHMcc(delta16 PHM 356s)

Plasmid

#25455

Depositor

Betty Eipper

Article

Kolhekar et al Biochemistry. 1997 Sep 9. 36(36):10901-9

Insert

PHM (Pam Rat)

Expression

Mammalian

Mutation

Contains the catalytic core of PHM, including aa …

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pAct:MCP-VP64

Plasmid

#78904

Purpose

Expresses MCP:VP64 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

MS2:VP64

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

March 25, 2017

Availability

Academic Institutions and Nonprofits only

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