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pRO16Sh2

Plasmid

#180604

Purpose

Expression of ShCAST under control of Plac with pRO1600 origin.

Depositor

Christopher Reisch

Publication

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLac

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCBP60g-NLS-3xmCitrine

Plasmid

#192521

Purpose

Fluorescent reporter for plant responses to bacterial infection

Depositor

Gitta Coaker

Publication

Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

Insert

CALMODULIN BINDING PROTEIN 60g

Tags

3xmCitrine and NLS

Expression

Plant

Promoter

CBP60g

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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YFP-Stim1-D76A

Plasmid

#186622

Purpose

Encodes Stim1 mutant D76A fused to YFP

Depositor

Gia Voeltz

Publication

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Stromal Interaction Molecule 1 (STIM1 Human)

Tags

YFP

Expression

Mammalian

Mutation

D76A

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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YFP-Stim1-ΔCTD

Plasmid

#186621

Purpose

Encodes Stim1 with C-terminal domain deletion fused to YFP

Depositor

Gia Voeltz

Publication

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Stromal Interaction Molecule 1 (STIM1 Human)

Tags

YFP

Expression

Mammalian

Mutation

Deletion of C-terminal domain 601 to 685

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pSelect-6

Plasmid

#190243

Purpose

Encodes lacI- and GFP-targeting gRNAs for positive and negative selections of dCas9 activity.

Depositor

Marc Ostermeier

Publication

Spisak et al PLoS One. 2022 Jun 3;17(6):e0269270. doi

Insert

LacI

Expression

Bacterial

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEA106

Plasmid

#187872

Purpose

Expresses mCherry under the virB promoter.

Depositor

Kan Wang

Publication

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Insert

mCherry

Expression

Bacterial

Promoter

Agrobacterium virB promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEA183

Plasmid

#187873

Purpose

Expresses eGFP under the virB promoter.

Depositor

Kan Wang

Publication

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Insert

eGFP

Expression

Bacterial

Promoter

Agrobacterium virB promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pFTR5

Plasmid

#167507

Purpose

Broad-Host range expression vector, RSF1010 origin, TetR repressor, PTetA promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Publication

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

TetR-RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTetA

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Gdf9

Plasmid

#163599

Purpose

Expresses GDF-9 in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf9 (Gdf9 Mouse)

Expression

Mammalian

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pFVK5

Plasmid

#167522

Purpose

Broad-Host range expression vector, RSF1010 origin, VanRAM repressor, PVanCC promoter, mKelly2, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Publication

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanRAM-mKelly2

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pBVR5

Plasmid

#167511

Purpose

Broad-Host range expression vector, pBBR origin, VanRAM repressor, PVanCC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Publication

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanRAM-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFTtR5

Plasmid

#167508

Purpose

Broad-Host range expression vector, RSF1010 origin, TtgRAM repressor, PTtg promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Publication

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

TtgRAM-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTtg

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFRG5

Plasmid

#149486

Purpose

Broad-Host range expression vector, RSF1010 origin, RhaR-RhaS activator, PrhaBAD promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Publication

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

RhaS-RhaR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrhaBAD

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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OA-1050E (Ubiq-CasRx)

Plasmid

#132416

Purpose

Express CasRx under Ubiquitin-63E promoter

Depositor

Omar Akbari

Publication

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

CasRx

Expression

Insect

Promoter

Ubiq

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDCB014

Plasmid

#135433

Purpose

Yeast integrating plasmid to express MCP-ABI and PYL1-VP64

Depositor

Jesse Zalatan

Publication

Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

Inserts

MCP-ABI
PYL1-VP64

Expression

Yeast

Promoter

pAdh1 and pHHF2

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pNM3749

Plasmid

#154108

Purpose

loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

Depositor

Michael Nonet

Publication

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Inserts

LoxP-SEC-LoxP
FRT::rpl-28p::GFP::his-58::FRT3

Use

Cre/Lox; Rmce

Expression

Worm

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJZC533

Plasmid

#135435

Purpose

Yeast integrating plasmid for pTEF1-mCherry reporter gene

Depositor

Jesse Zalatan

Publication

Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

Insert

mCherry

Expression

Yeast

Mutation

codon optimized for S. cerevisiae

Promoter

pTEF1

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJFRC7-UAS-NPRR_dTK

Plasmid

#133244

Purpose

Fluorescent reporter for neuropeptide release imaging

Depositor

David Anderson

Publication

Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

Insert

drosophila tachykinin-fused codon-optimized GCaMP6s

Expression

Insect

Promoter

hsp70

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pKIF1C-BioID2-HA

Plasmid

#130980

Purpose

Expression of HA-tagged KIF1C-BioID2

Depositor

Anne Straube

Publication

Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

Insert

KIF1C-BioID2-HA (KIF1C Human)

Tags

BioID2-HA

Expression

Mammalian

Mutation

5 silent mutations that make this construct RNAi …

Promoter

CMV

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1820_pCR8-DR-RG6-SA-DR

Plasmid

#118654

Purpose

Transient transfection; Expressed DR-RG6-SA-DR CasRx gRNA with preprocessed DR 3'; Gateway Donor

Depositor

Albert Cheng

Publication

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

DR-RG6-SA-DR

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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