We narrowed to 5,822 results for: pCas

1. pX330-UGCG-KO

   Plasmid

   #80010

   Purpose

   gRNA to knock out expression of UGCG gene. The product of this gene catalyzes the first step in synthesis of glycosphingolipids.

   Depositor

   Sriram Neelamegham

   Article

   Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

   Insert

   UGCG (UGCG Human)
   

   Use

   CRISPR

   Available Since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. BPK1179

   Plasmid

   #179296

   Purpose

   Expresses dCas9 fused to four DmrA domains

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

   Insert

   dCas9-DmrA(x4)
   

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   CAG

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRB14

   Plasmid

   #52522

   Purpose

   expresses a myc-tagged version of hCas9 in Drosophila

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   S. pyogenes cas9 with humanized codon bias
   

   Tags

   myc

   Expression

   Insect

   Promoter

   tubulin

   Available Since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPBT-PE2-PGK-Blast

   Plasmid

   #173219

   Purpose

   piggyBac transposon containing PE2 and Blasticidin expression cassettes

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

   Inserts

   PE2
   Blast
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   CMV and hPGK

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. iCAP-BI155-KanR

   Plasmid

   #209527

   Purpose

   RepCap for AAV production

   Depositor

   Ben Deverman

   Article

   Eid et al Nat Commun. 2024 Aug 4;15(1):6602. doi:

   Insert

   AAV-BI155 Cap
   

   Expression

   Mammalian

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Fyn-Fyn Biosensor

   Plasmid

   #124655

   Purpose

   FRET biosensor. Fyn-tagged biosensor targeting to membrane

   Depositor

   Yingxiao Wang

   Article

   Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

   Insert

   Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
   

   Expression

   Mammalian

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMK-Cas9-gate

   Plasmid

   #113743

   Purpose

   Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving aminoglycoside phosphotransferase for G418 selection in plants.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   Cas9
   

   Use

   CRISPR; Gateway destination vector

   Promoter

   Z. Mays ubiquitin promoter, 35S promoter

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-eCas9-sgMIB1

   Plasmid

   #140239

   Purpose

   Lentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1

   Depositor

   Urs Greber

   Article

   Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

   Insert

   sgRNA targeting human MIB1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. EE-BE4max

   Plasmid

   #138158

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   rAPOBEC1(EE)-nSpCas9-UGI-UGI
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Within rAPOBEC1 R126E + R132E; within Cas9 D10A

   Promoter

   CMV

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-DIO_EGFP-RandE3

    Plasmid

    #79872

    Purpose

    For generating adeno-associated virus to express RandE3 under the EF1a promoter in a 'Cre-on' dependent manner.

    Depositor

    Don Arnold

    Article

    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

    Insert

    GFP-RandE3
    

    Use

    AAV, Cre/Lox, and Synthetic Biology

    Tags

    GFP and HA

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry

    Plasmid

    #170182

    Purpose

    Mammalian expression vector for sgRNAs cloned into the BbsI sites and for expression of spCas9 linked to mCherry via a T2A peptide

    Depositor

    Hunter Fraser

    Article

    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

    Insert

    mCherry
    

    Tags

    3XFlag and T2A

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. px330-UFM1 sgRNA2

    Plasmid

    #134634

    Purpose

    contains sgRNA targeting human UFM1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFM1 sgRNA2 (UFM1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. px330-UFM1 sgRNA1

    Plasmid

    #134633

    Purpose

    contains sgRNA targeting human UFM1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFM1 sgRNA1 (UFM1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEn-C1.1

    Plasmid

    #61479

    Purpose

    Encodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPC

    Depositor

    Holger Puchta

    Article

    Schiml et al Plant J. 2014 Oct 18. doi: 10.1111/tpj.1

    Insert

    AtU6-26:sgRNA
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    U6-26

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. BPK1169: CAG-DmrC-NLS-3xFLAG-P65

    Plasmid

    #104564

    Purpose

    Mammalian expression vector for DmrC fused to p65 activation domain

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    DmrC-p65
    

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC1300_pUB10_pcoCASphi_E9t_version2_U6_AtPDS3_gRNA10

    Plasmid

    #197952

    Purpose

    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal , and the AtPDS3 guide RNA 10 driven by U6 promoter.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Inserts

    pcoCasphi-2
    AtPDS3 gRNA10
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU6-26 and pUBQ10

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. TDP43 NOTAG6

    Plasmid

    #28209

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    aa1-314 only

    Available Since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pUC57-Amp_CRISPIE donor B3

    Plasmid

    #172844

    Purpose

    CRISPIE donor B3 (Zhong et al, eLife 2021), mTurquoise2 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    CRISPIE designer exon (phase 0-0) encoding mTurquoise2
    

    Use

    CRISPR

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC57-Amp_CRISPIE donor B4

    Plasmid

    #172845

    Purpose

    CRISPIE donor B4 (Zhong et al, eLife 2021), mNeonGreen translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    CRISPIE designer exon (phase 0-0) encoding mNeonGreen
    

    Use

    CRISPR

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. px330-UFL1 sgRNA2

    Plasmid

    #134636

    Purpose

    contains sgRNA targeting human UFL1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFL1 sgRNA2 (UFL1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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