Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 28,960 results for: Tat

Showing: 9761 - 9800 of 28960 results

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mCherry-FKBP-PIP4K2AD273K

Plasmid

#202733

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:PIP4K2A (D266K) (PIP4K2A, FKBP1A Human)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-RPE_sgRNA1

Plasmid

#201618

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

RPE (RPE Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(703-868, P801G)

Plasmid

#180029

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G). P801G mutation facilitates expression in E. coli.

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-ALIX(703-868, P801G) (PDCD6IP Human)

Tags

GB1-His6-TEV

Expression

Bacterial

Mutation

P801G

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK BirA* 3xFLAG-CSE1L zeo

Plasmid

#192334

Purpose

Lentiviral expression vector for an inducible 3xFLAG-BirA 3xFLAG-CSE1L

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

BirA*-3xFLAG-CSE1L (CSE1L Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT BirA*-3xFLAG-CSE1L

Plasmid

#192312

Purpose

Gateway entry vector for an inducible 3xFLAG-BirA* 3xFLAG-CSE1L

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

BirA*-3xFLAG-CSE1L (CSE1L Human)

Use

Gateway entry vector

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe NGF-EGFR-FRB-GluGlu neo

Plasmid

#192293

Purpose

Retroviral expression vector for NGF-EGFR-FRB-GluGlu construct

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

NGF-EGFR-FRB-GluGlu (EGFR Human)

Use

Retroviral

Tags

GluGlu

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe NGF-Erbb2-2xFKBP-HA hygro

Plasmid

#192291

Purpose

Retroviral expression vector for NGF-Erbb2-2xFKBP-HA construct

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

NGF-Erbb2-2xFKBP-HA (Erbb2 Rat)

Use

Retroviral

Tags

HA

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_hCOL4A5-Nluc_S1632X

Plasmid

#198122

Purpose

Express hCOL4A5_S1632X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Tags

Nluc

Expression

Mammalian

Mutation

S1632X

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_hCOL4A5-Nluc_Q1016X

Plasmid

#198119

Purpose

Express hCOL4A5_Q1016X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Tags

Nluc

Expression

Mammalian

Mutation

Q1016X

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_hCOL4A5-Nluc_G5X

Plasmid

#198114

Purpose

Express hCOL4A5_G5X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Tags

Nluc

Expression

Mammalian

Mutation

G5X

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_hCOL4A5-Nluc_C29X

Plasmid

#198115

Purpose

Express hCOL4A5_C29X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Tags

Nluc

Expression

Mammalian

Mutation

C29X

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_hCOL4A5-Nluc_E130X

Plasmid

#198117

Purpose

Express hCOL4A5_E130X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Tags

Nluc

Expression

Mammalian

Mutation

E130X

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_hCOL4A5-Nluc_S36X

Plasmid

#198116

Purpose

Express hCOL4A5_S36X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Tags

Nluc

Expression

Mammalian

Mutation

S36X

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_hCOL4A5-Nluc_S1661X

Plasmid

#198123

Purpose

Express hCOL4A5_S1661X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Tags

Nluc

Expression

Mammalian

Mutation

S1661X

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 PSK (745-1235)

Plasmid

#197863

Purpose

Expression of C-terminal PSK1 / TAOK2 (aa745-1235) in mammalian cells (microtubule-binding domain)

Depositor

Jonathan Morris

Article

Mitsopoulos et al J Biol Chem. 2003 May 16;278(20):18085-9

Insert

PSK1 (745-1235) (TAOK2 Human)

Tags

Myc

Expression

Mammalian

Mutation

The N terminus of PSK1/TAOK2 has been deleted

Promoter

SV40

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-PSK2 (K57A)

Plasmid

#197118

Purpose

Expression of kinase-defective MYC-tagged PSK2 (K57A) / TAOK1 (K57A) in mammalian cells

Depositor

Jonathan Morris

Article

Zihni et al J Biol Chem. 2006 Mar 17;281(11):7317-23

Insert

TAOK1 (K57A) (TAOK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Changed K 57 to A

Promoter

SV40

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-mut4/1_2_VB

Plasmid

#186444

Purpose

Encodes Cas4(K81A)/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1 and Cas2

Use

CRISPR

Mutation

Cas4 domain (K81A)

Promoter

T7

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsNot1iso1_1093-2376_AE

Plasmid

#148629

Purpose

Bacterial Expression of HsNot1iso1_1093-2376

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsNot1iso1_1093-2376 (CNOT1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsNot1iso1_1607-1815_AE

Plasmid

#148631

Purpose

Bacterial Expression of HsNot1iso1_1607-1815

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsNot1iso1_1607-1815 (CNOT1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpH-HsNot1_1093-1317_AF

Plasmid

#148699

Purpose

Bacterial Expression of HsNot1_1093-1317

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsNot1_1093-1317 (CNOT1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-GST-DmRoquin_702-819-del725-755-del790-812_AE

Plasmid

#148606

Purpose

Insect Expression of DmRoquin_702-819-del725-755-del790-812

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin_702-819-del725-755-del790-812 (roq Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-DmRoquin_702-819-del725-755-del790-812-GB1His6_AE

Plasmid

#148621

Purpose

Bacterial Expression of DmRoquin_702-819-del725-755-del790-812

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin_702-819-del725-755-del790-812 (roq Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpH-HsNot3_607-748-NpM-HsNot2_350-540_W

Plasmid

#147909

Purpose

Bacterial Expression of HsNot3_607-748-HsNot2_350-540

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsNot3_607-748-HsNot2_350-540 (CNOT3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCCR4_168-552-D412AN414A_R

Plasmid

#147427

Purpose

Insect Expression of DmCCR4_168-552-D412AN414A

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4_168-552-D412AN414A (twin Fly)

Expression

Insect

Mutation

4 silent mutations and 1 non silent mutations com…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmGW182_539-830_O

Plasmid

#147150

Purpose

Insect Expression of DmGW182_539-830

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182_539-830 (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmGW182_539-1115_O

Plasmid

#147151

Purpose

Insect Expression of DmGW182_539-1115

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182_539-1115 (gw Fly)

Expression

Insect

Mutation

two non silent mutations V704A and N799S, and one…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmGW182-SD6Wmut_O

Plasmid

#147155

Purpose

Insect Expression of DmGW182-SD6Wmut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182-SD6Wmut (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmGW182_604-1115_N

Plasmid

#147060

Purpose

Insect Expression of DmGW182_604-1115

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182_604-1115 (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmGW182_604-1384_N

Plasmid

#147061

Purpose

Insect Expression of DmGW182_604-1384

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182_604-1384 (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmGW182_635-1384_N

Plasmid

#147062

Purpose

Insect Expression of DmGW182_635-1384

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182_635-1384 (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM-41P-DmGW182_744-817_N

Plasmid

#147064

Purpose

Bacterial Expression of DmGW182_744-817

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182_744-817 (gw Fly)

Expression

Bacterial

Mutation

one non silent mutations N799S and one silent mut…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmGW182_1-824_N

Plasmid

#147059

Purpose

Insect Expression of DmGW182_1-824

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182_1-824 (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmCCR4_168-552_I

Plasmid

#146612

Purpose

Insect Expression of DmCCR4_168-552

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4_168-552 (twin Fly)

Expression

Insect

Mutation

4 silent mutations and 1 non silent mutations com…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmCCR4_168-552_I

Plasmid

#146603

Purpose

Insect Expression of DmCCR4_168-552

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4_168-552 (twin Fly)

Expression

Insect

Mutation

4 silent mutations and 1 non silent mutations com…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmGW182_1-830-dsRNAres_G

Plasmid

#146388

Purpose

Insect Expression of DmGW182_1-830-dsRNAres

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmGW182_1-830-dsRNAres (gw Fly)

Expression

Insect

Mutation

two non silent mutations V704A and N799S, and one…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmGW182_1-1115-dsRNAres_G

Plasmid

#146389

Purpose

Insect Expression of DmGW182_1-1115-dsRNAres

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmGW182_1-1115-dsRNAres (gw Fly)

Expression

Insect

Mutation

two non silent mutations V704A and N799S, and one…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmMe31B-Q281AH284A_E

Plasmid

#146204

Purpose

Insect Expression of DmMe31B-Q281AH284A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmMe31B-Q281AH284A (me31B Fly)

Expression

Insect

Mutation

one silent mutation A1101G and two non silent mut…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmMe31B-T288AK292A_E

Plasmid

#146205

Purpose

Insect Expression of DmMe31B-T288AK292A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmMe31B-T288AK292A (me31B Fly)

Expression

Insect

Mutation

one silent mutation A1101G and two non silent mut…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsSmg7-G100E-siRNAres-shRNAres_S

Plasmid

#147461

Purpose

Mammalian Expression of HsSmg7-G100E-siRNAres-shRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-G100E-siRNAres-shRNAres (SMG7 Human)

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C and A1521G an…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsSmg7-K66ER163E-siRNAres-shRNAres_S

Plasmid

#147462

Purpose

Mammalian Expression of HsSmg7-K66ER163E-siRNAres-shRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-K66ER163E-siRNAres-shRNAres (SMG7 Human)

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C and A1521G an…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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