We narrowed to 13,725 results for: cas9

1. pCSW24

   Plasmid

   #86837

   Purpose

   bacterial expression of anti-CRISPR protein AcrIIA3

   Depositor

   Joseph Bondy-Denomy

   Article

   Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

   Insert

   acrIIA3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Oct. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCSW65

   Plasmid

   #86838

   Purpose

   bacterial expression of anti-CRISPR protein AcrIIA3

   Depositor

   Joseph Bondy-Denomy

   Article

   Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

   Insert

   acrIIA3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAP639-2F

   Plasmid

   #99486

   Purpose

   3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAEF6

   Plasmid

   #136303

   Purpose

   pAEF5 plasmid with two gRNA inserted

   Depositor

   Gilles Fischer

   Article

   Agier et al Methods Mol Biol. 2021;2196:181-198. doi

   Insert

   double cassette coding for gRNA
   

   Use

   CRISPR; Template for pcr to insert double grna co…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAP683-2

   Plasmid

   #99488

   Purpose

   TEV::eGFP::ER::HA::linker::3XFlag

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   TEV::eGFP::RE::HA::linker::3XFlag
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAP607-2

   Plasmid

   #99483

   Purpose

   Myc::TEV::3XFlag::GFP::3XFlag::TEV::Myc

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   Myc::TEV::3XFlag::GFP::3XFlag::TEV::Myc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pWT019b

   Plasmid

   #107894

   Purpose

   W1.2 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1, PRha-sgRNA2 (sgRNA1-G19T-G20C) and is part of CAMERA 1.2

   Depositor

   David Liu

   Article

   Tang et al Science. 2018 Feb 15. pii: science.aap89

   Insert

   PTetO-sd2U-Cas9, PLacO-sgRNA1, PRha-sgRNA2 (sgRNA1-G19T-G20C)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BCJJ223

   Plasmid

   #117509

   Purpose

   AtMGE1 promoter Golden Gate Level 0

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BsaI:GGAG:pMGE1:AATG:BsaI
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtMGE1

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pICSL12021

   Plasmid

   #117511

   Purpose

   AtICU2 promoter Golden Gate Level 0

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BsaI:GGAG:pICU2:AATG:BsaI
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtICU2

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAP684-3

    Plasmid

    #99489

    Purpose

    TEV::eGFP::ER::Ollas::linker::3XFlag

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    TEV::eGFP::RE::Ollas::linker::3XFlag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJAT22

    Plasmid

    #204311

    Purpose

    For generating targeting inversions. N-terminal split GFP, expressed in eyes.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-GFPn
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    3XP3

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. psBIG1a ActBT2ATetP2AP - mAGH

    Plasmid

    #229973

    Purpose

    Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and mAGNLS-T2A-Hygro

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    ActBT2ATetP2AP - mAGH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. psBIG1a ActBT2ATetP2AP - H

    Plasmid

    #229972

    Purpose

    Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and Hygro

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    ActBT2ATetP2AP - H
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. psBIG1a ActBT2AmcherryP2AP -TagBFPH

    Plasmid

    #229971

    Purpose

    Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains Last ActB exon fused T2A-mCherry-P2A-puro and mTagBFPnls-T2A-Hygro

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    ActBT2AmcherryP2AP -TagBFPH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-CMV-dSa VP64 Afp

    Plasmid

    #99697

    Purpose

    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Afp promoter, vector allows for activation of mouse Afp, can be packaged and delivered as AAV

    Depositor

    George Church

    Insert

    dCas9 and gRNA targeting Afp (Afp Synthetic)
    

    Use

    AAV and CRISPR

    Tags

    VP64

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC57-ITR2-H1-sgCTG-SlugD10A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #222857

    Purpose

    This plasmid codes for the Slug-KH nickase with a sg(CTG)6

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    Slug-KHD10A+sgCTG
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, ITR2 and ITR2

    Expression

    Mammalian

    Mutation

    KH variant and D10A mutation

    Promoter

    H1 and eCMV

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC57-ITR2-H1-sgAGC-SlugD10A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #222859

    Purpose

    This plasmid codes for the Slug-KH nickase with a sg(AGC)6

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    Slug-KHD10A+sgAGC
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, ITR2 and ITR2

    Expression

    Mammalian

    Mutation

    KH variant and D10A mutation

    Promoter

    H1 and eCMV

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC57-ITR2-H1-sgCAG-SlugD10A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #222858

    Purpose

    This plasmid codes for the Slug-KH nickase with a sg(CAG)6

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    Slug-KHD10A+sgCAG
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, ITR2 and ITR2

    Expression

    Mammalian

    Mutation

    KH variant and D10A mutation

    Promoter

    H1 and eCMV

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC57-ITR2-H1-sgGCA-SlugD10A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #222860

    Purpose

    This plasmid codes for the Slug-KH nickase with a sg(GCA)6

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    Slug-KHD10A+sgGCA
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, ITR2 and ITR2

    Expression

    Mammalian

    Mutation

    KH variant and D10A mutation

    Promoter

    H1 and eCMV

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMLS713

    Plasmid

    #188885

    Purpose

    Multisite Gateway [4-3] Destination vector; miniMos transposon with lox2272-flanked Cbr-unc-119 marker

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Type

    Empty backbone

    Use

    Multisite gateway [4-3] destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge