We narrowed to 19,963 results for: Kin

1. pHHM.X513-iNicSnFR2-(CC90)

   Plasmid

   #124836

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

   Depositor

   Henry Lester

   Article

   Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

   Insert

   iNicSnFR2-(CC90)
   

   Use

   Tags

   HA tags and His Tag

   Expression

   Bacterial

   Mutation

   NA

   Promoter

   T7

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PAK4-FL-3'UTR-MT2

   Plasmid

   #113093

   Purpose

   Luciferase reporter containing PAK4-FL- 3' UTR with the second predicted MiR-199a-3p binding site mutated

   Depositor

   James Donahue

   Article

   Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

   Insert

   p21 Activated kinase 4 (PAK4 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Second predicted miR-199a-3p binding site mutated…

   Promoter

   PGK

   Available Since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. PAK4-FL-3'UTR-MT-both

   Plasmid

   #113094

   Purpose

   Luciferase reporter containing PAK4-FL- 3' UTR with both the predicted MiR-199a-3p binding sites mutated

   Depositor

   James Donahue

   Article

   Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

   Insert

   p21 Activated kinase 4 (PAK4 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Both the predicted miR-199a-3p binding site muta…

   Promoter

   PGK

   Available Since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a_6HT-TBP6.7(R47A)

   Plasmid

   #112722

   Purpose

   Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R47A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R47A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a_6HT-TBP6.7(R47K)

   Plasmid

   #112723

   Purpose

   Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R47K)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R47K

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a_6HT-TBP6.7(Q48A)

   Plasmid

   #112724

   Purpose

   Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q48A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q48A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a_6HT-TBP6.7(T50A)

   Plasmid

   #112727

   Purpose

   Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(T50A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   T50A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a_6HT-TBP6.7(R52A)

   Plasmid

   #112729

   Purpose

   52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R52A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R52A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-30a(+)-C-SH2

   Plasmid

   #111419

   Purpose

   express murine Syk (C)SH2 domain (His 162 to Gln 264), in E.coli strain Rosetta 2 (DE3)

   Depositor

   Carol Post

   Article

   Feng et al Protein Sci. 2018 Jul 27. doi: 10.1002/p

   Insert

   murine Syk (C)SH2 domain (His 162 to Gln 264), without any tag (Syk Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-30a(+)-C-SH2+IA

    Plasmid

    #111418

    Purpose

    express murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), in E.coli strain Rosetta 2 (DE3)

    Depositor

    Carol Post

    Article

    Feng et al Protein Sci. 2018 Jul 27. doi: 10.1002/p

    Insert

    murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), without any tag (Syk Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. NDUFAB1 C3.3 gRNA

    Plasmid

    #90791

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NDUFAB1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    NDUFAB1 (Guide Designation C3.3) (NDUFAB1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. NDUFAB1 C4.3 gRNA

    Plasmid

    #90793

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NDUFAB1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    NDUFAB1 (Guide Designation C4.3) (NDUFAB1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcdna3 T7 Jip1b(471-707)(C478S and C699T)

    Plasmid

    #52132

    Purpose

    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

    Insert

    JNK interacting protein-1b (JIP-1b)(471-707)(M1+6 ) (Mapk8ip1 Mouse)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted amino acid 1-470 and changed C478S and C6…

    Promoter

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pcdna3 T7 Jip1b(282-470)

    Plasmid

    #52125

    Purpose

    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

    Insert

    JNK interacting protein-1b (JIP-1b)(282-470) (Mapk8ip1 Mouse)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted amino acid 1-281 and 471-707

    Promoter

    Available Since

    April 18, 2014

    Availability

    Academic Institutions and Nonprofits only

15. RpH-ILV-3xALFA

    Plasmid

    #225135

    Purpose

    RpH-ILV probe strongly colocalises with lysosomal markers.

    Depositor

    Massimiliano Stagi

    Article

    Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

    Insert

    TMEM192 (TMEM192 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMG046 pETARA-CK1α-His

    Plasmid

    #154074

    Purpose

    Expresses GST_CK1α (human CK1α as a fusion protein with GST and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    CSNK1A1 (CSNK1A1 Human)
    

    Use

    Tags

    GST and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. MtPT4-p3

    Plasmid

    #160003

    Purpose

    Multigene construct containing the betalain pigment biosynthetic genes under the control of the MtPT4 promoter for visualisation of arbuscular mycorrhizal colonisation in Medicago truncatula roots.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Inserts

    DsRed (reverse orientation)
    DODAα1
    CYP76AD1
    cDOPA5GT
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Arabidopsis thaliana Ubiquitin 10 Promoter (AtUb1…

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiPGK Blast DEST JNKKTRmRuby2

    Plasmid

    #59154

    Purpose

    Lentiviral vector to express JNK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    JNK Kinase Translocation Reporter (MAPK8 Human)
    

    Use

    Lentiviral

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIG-602_NY-ESO-1_TCR_RFP

    Plasmid

    #207483

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    RFP, NY-ESO-1_TCR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn1-DIO-ExRai-AKAR2

    Plasmid

    #171846

    Purpose

    Cre-dependent, synapsin promoter-driven expression of the green fluorescent ExRai-AKAR2 PKA biosensor in neurons.

    Depositor

    Richard Huganir

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    ExRai-AKAR2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available Since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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