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pAAV-Linker-hSyn::mCherry.3xFLAG-WPRE

Plasmid

#120391

Purpose

pAAV plasmid expressing mCherry.3XFLAG under the hSyn promoter with a Linker containing BstBI, BspQI and SacI sites upstream of the hSyn promoter

Depositor

Florian Freudenberg

Publication

Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

Type

Empty backbone

Use

AAV

Tags

mCherry.3XFLAG

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pORANGE GFP-Camk2a KI

Plasmid

#131484

Purpose

Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA and GFP donor (Camk2a, Camk2a Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PX429 SpCas9 N863A nickase

Plasmid

#68706

Purpose

SpCas9 N863A nickase

Depositor

Feng Zhang

Publication

Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9 N863A nicking mutant

Use

CRISPR

Expression

Mammalian

Promoter

Cbh

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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GCEPIA1-SNAPER

Plasmid

#172659

Purpose

Detect the real-time ER calcium store and local calcium microdomains in live cells.

Depositor

Pingyong Xu

Publication

Luo et al Biophys Rep (2019) 5, 31–42

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

calmodulin

Expression

Mammalian

Mutation

Please see depositor comments

Available since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-CMV-GFP-pA U6 shRNA (Rat Nurr1 #1)

Plasmid

#233273

Purpose

To express GFP from a CMV promoter and to express an shRNA targeting Rat Nurr1

Depositor

Jonathan Ploski, Glenn Schafe

Insert

GFP and shRNA targeting Rat Nurr1 (Nr4a2 Rat)

Use

AAV

Available since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-GFP-pA U6 shRNA (Rat Nurr1 #5)

Plasmid

#233274

Purpose

To express GFP from a CMV promoter and to express an shRNA targeting Rat Nurr1

Depositor

Jonathan Ploski, Glenn Schafe

Insert

GFP and shRNA targeting Rat Nurr1 (Nr4a2 Rat)

Use

AAV

Available since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-tsC2ProT-mScarlet3-Cre

Plasmid

#248522

Purpose

This vector co-expresses Red Fluorescent Protein mScarlet3 and Cre Recombinase under the control of the truncated Tree Shrew Cerebellin 2 Promoter.

Depositor

Alev Erisir

Publication

Kipcak et al Cell Rep Methods. 2026 Mar 23;6(3):10130

Insert

Truncated Tree Shrew Cerebellin 2 Promoter + mScarlet3 + Cre

Use

AAV

Expression

Mammalian

Promoter

Truncated Tree shrew Cerebellin 2 Promoter

Available since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-DIO-LifeCamp

Plasmid

#251726

Purpose

Expression of Cre-dependent calcium lifetime sensor LifeCamp under hSyn promoter in pAAV backbone

Depositor

Bernardo Sabatini

Publication

Lodder et al bioRxiv 2025.12.23.696288

Insert

LifeCamp

Use

AAV

Expression

Mammalian

Available since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-RiboL1-LifeCamp

Plasmid

#251728

Purpose

Expression of soma-targeted calcium lifetime sensor LifeCamp under hSyn promoter in pAAV backbone

Depositor

Bernardo Sabatini

Publication

Lodder et al bioRxiv 2025.12.23.696288

Insert

RiboL1-LifeCamp

Use

AAV

Expression

Mammalian

Available since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-HyPerFLEX-Sec61b

Plasmid

#238940

Purpose

Mammalian expression of HyPerFLEX targeted to the cytosolic side of the endoplasmic reticulum membranes

Depositor

Vsevolod Belousov

Publication

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-Sec61b

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-pAceR-Kv2.1PR

Plasmid

#195531

Purpose

Red fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

Depositor

Vincent Pieribone

Publication

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAceR-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

VARNAM 78E, 81D, 92N; SI linker

Promoter

CaMKII

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-pCaMKII-EBFP-tTAn

Plasmid

#172124

Purpose

Expression of EBFP and tTA-InteinN in CaMKII positive cells

Depositor

Chun Xu

Publication

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

pCaMKII, EBFP, tTAn-InteinN

Use

AAV

Promoter

EF1a

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-GFP-mirNega

Plasmid

#163705

Purpose

pAAV plasmid to induce expression of universal negative control micro-RNA and EGFP

Depositor

Michisuke Yuzaki

Publication

Ito-Ishida et al J Neurosci. 2020 Nov 4;40(45):8746-8766.

Insert

mirNega and EGFP

Use

AAV

Promoter

CAG

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1alpha-soCoChR-GFP

Plasmid

#107709

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter

Depositor

Edward Boyden

Publication

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Insert

soCoChR-GFP

Use

AAV

Tags

EGFP and KA2

Expression

Mammalian

Promoter

EF1alpha

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-REX-GECO0.9

Plasmid

#61249

Purpose

Expresses REX-GECO0.9 in neurons

Depositor

Robert Campbell

Publication

Wu et al Nat Commun. 2014 Oct 31;5:5262. doi: 10.

Insert

REX-GECO0.9

Use

AAV

Expression

Mammalian

Mutation

Substitutions relative to R-GECO1: P60R, V61W, R6…

Promoter

hSyn

Available since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV GFP

Plasmid

#213685

Purpose

Expresses Green Fluorescent Protein (GFP) with an N-terminal twin-Strep tag

Depositor

Thomas Kukar

Publication

Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

Insert

Twin-Strep GFP

Use

AAV

Tags

Twin-Strep

Promoter

cytomegalovirus enhancer/chicken β-actin promoter

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn-FLEX.iGABASnFR.F102Y.Y137L.mRuby3

Plasmid

#112171

Purpose

GABA Sensor

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102Y.Y137L

Use

AAV

Tags

mRuby3

Mutation

F102Y.Y137L

Promoter

Synapsin

Available since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-sCreDIO hChR2(H134R)-EYFP

Plasmid

#55642

Purpose

sCre-Dependent ChR2-EYFP

Depositor

Karl Deisseroth

Publication

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

hChR2(H134R)-EYFP

Use

AAV; Scre-dependent

Tags

EYFP

Expression

Mammalian

Promoter

Ef1a

Available since

Aug. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-F-RAM-CytoTape-V5

Plasmid

#239427

Purpose

CytoTape signal monomer for recording Fos protein activity

Depositor

Changyang Linghu

Publication

Zheng et al Nature. 2026 Jan 26. doi: 10.1038/s41586

Insert

CytoTape-V5

Use

AAV

Tags

V5-dMBP

Expression

Mammalian

Promoter

F-RAM promoter

Available since

Jan. 7, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-UbC-CytoTape-HA

Plasmid

#239423

Purpose

CytoTape structural monomer for CytoTape fiber formation and elongation for cell culture applications

Depositor

Changyang Linghu

Publication

Zheng et al Nature. 2026 Jan 26. doi: 10.1038/s41586

Insert

CytoTape-HA

Use

AAV

Tags

HA-dMBP

Expression

Mammalian

Promoter

UbC promoter

Available since

Nov. 12, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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