We narrowed to 8,319 results for: construct

1. CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

   Plasmid

   #225756

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC18-mini-Tn7T-Gm-LacZ10

   Plasmid

   #65026

   Purpose

   GmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 1. See Depositor Comments below for information regarding the lacZ sequence.

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Type

   Empty backbone

   Use

   Tags

   lacZ

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. LT1_33_pTEFmut8-mCherry

   Plasmid

   #177706

   Purpose

   Construct for genome integration expressing constitutively mCherry under pTEFmut8 promoter

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Insert

   pTEFmut8-mCherry
   

   Use

   Used as donor dna in genome integration after lin…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBPGUw-HACK-G4>QF2

   Plasmid

   #80277

   Purpose

   GAL4->QF2 HACK construct compatible with GMR attP integrated insertions. Contains 5' and 3' GAL4 homology arms and gRNAs.

   Depositor

   Christopher Potter

   Article

   Lin et al Genetics. 2016 Jun 22. pii: genetics.116

   Insert

   QF2
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMAH2-intein/mCherry

   Plasmid

   #71405

   Purpose

   Expresses suppression tRNA and mCherry inserted with a Npu DnaE intein

   Depositor

   Huiwang Ai

   Article

   Ren et al J Am Chem Soc. 2015 Feb 18;137(6):2155-8

   Insert

   Npu DnaE Intein insertion in mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

6. zCREST3:redCas9; control_gRNA

   Plasmid

   #199334

   Purpose

   pDEL157; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous control sgRNA

   Depositor

   William Talbot

   Article

   Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

   Inserts

   zCREST3
   Cas9
   control sgRNA
   

   Use

   Tol2 destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmNFL

   Plasmid

   #83127

   Purpose

   Mouse neurofilament protein L cDNA mammalian expression construct

   Depositor

   Anthony Brown

   Article

   Yan et al Cell Motil Cytoskeleton. 2007 Apr;64(4):

   Insert

   mouse NFL (Nefl Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)

   Plasmid

   #225755

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)

   Plasmid

   #225754

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pQCXIP-GFP-LacR

    Plasmid

    #59418

    Purpose

    retroviral construct expressing the Lac repressor (LacR) tagged with GFP

    Depositor

    Tom Misteli

    Article

    Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

    Insert

    Lac repressor (LacR)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pSB_syb25

    Plasmid

    #197992

    Purpose

    Expressed sybody 25 as a C-terminal myc-His-tagged construct

    Depositor

    Simon Newstead

    Article

    Parker et al Nat Struct Mol Biol. 2023 Jul 23. doi: 1

    Insert

    Sybody 25
    

    Use

    Tags

    Myc-His

    Expression

    Bacterial

    Mutation

    Promoter

    Arabinose

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLC-284

    Plasmid

    #124294

    Purpose

    A low copy plasmid constitutively expressing vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.

    Depositor

    Swaine Chen

    Article

    Eshaghi et al Pathogens. 2016 Jan 5;5(1). pii: pathoge

    Insert

    vsfGFP-9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCI-myc-USP10

    Plasmid

    #184034

    Purpose

    Expresses myc-USP10 construct in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Jia et al J Biol Chem. 2021 Jan-Jun;296:100405. do

    Insert

    USP10 (USP10 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMVf

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQE-60NA-proinsulin-mEGFP

    Plasmid

    #160464

    Purpose

    Encodes a fusion of human proinsulin and the monomeric enhanced EGFP. This construct can be used as a negative control for superfolder activity in bacteria

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    proinsulin-mEGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTol2_LSEC:Cas9green; erbb2_gRNA171

    Plasmid

    #199338

    Purpose

    pDEL135; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous erbb2 sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    LSEC
    Cas9
    erbb2 sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SCP1-CMVenh-luc

    Plasmid

    #22216

    Depositor

    James Kadonaga

    Article

    Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

    Insert

    SCP1 (super core promoter 1)-CMVenh luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMTU-DO-G418-C4

    Plasmid

    #160342

    Purpose

    High-copy expression vector for K. marxianus, G418 selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPd4E-ISH

    Plasmid

    #61700

    Purpose

    Plum eIF4E ihp-RNA construct

    Depositor

    Lining Tian

    Article

    Wang et al PLoS One. 2013;8(1):e50627. doi: 10.1371

    Insert

    PdeIF4E
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    35S

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCI-NLS-BirA-2A-mCherry

    Plasmid

    #127781

    Purpose

    BirA construct in a PCI nls backbone driving mCherry activity

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    BirA
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZE31-DsRed-ms2x2

    Plasmid

    #26248

    Depositor

    Philippe Cluzel

    Article

    Guet et al Nucleic Acids Res. 2008 Jul . 36(12):e73

    Insert

    ms2 binding sites (x2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge