We narrowed to 17,708 results for: SHE

1. tRECs/3xUAS-FP attB

   Plasmid

   #220330

   Purpose

   encodes three Fluorescent Protein FLP-OUT cassettes responsive to three different recombinases (mFLP5, B3 and KD)

   Depositor

   Erdem Bangi

   Inserts

   B3RT-STOP-B3RT-TagBFP-3xHA
   KDRT-STOP-KDRT-GFP-HSV
   FRTm71-STOP-FRTm71-mCherry
   

   Expression

   Insect

   Promoter

   UAS

   Available Since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO-N-FLAG-hBirA*-TLK1-M566T

   Plasmid

   #218832

   Purpose

   Expresses N-terminal BirA*-tagged human TLK1 variant 5 mutant M566T for BioID experiments.

   Depositor

   Travis Stracker

   Insert

   TLK1 M566T (TLK1 Human)
   

   Tags

   FLAG-BirA*

   Expression

   Mammalian

   Mutation

   M566T

   Promoter

   CMV

   Available Since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO-N-FLAG-hBirA*-TLK1-Q479E

   Plasmid

   #218833

   Purpose

   Expresses N-terminal BirA*-tagged human TLK1 variant 5 mutant Q479E for BioID experiments.

   Depositor

   Travis Stracker

   Insert

   TLK1 Q479E (TLK1 Human)
   

   Tags

   FLAG-BirA*

   Expression

   Mammalian

   Mutation

   Q479E

   Promoter

   CMV

   Available Since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PE-SUMO CRYGA

   Plasmid

   #217672

   Purpose

   For expression of SUMO tagged human gamma A crystallin

   Depositor

   Larry David

   Insert

   CRYGA (CRYGA Human)
   

   Tags

   6XHis and Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-frt-DIO-hM3D(Gq)-mCherry

   Plasmid

   #217397

   Purpose

   Expresses the hM3D(Gq) excitatory DREADD fused to mCherry, in a Cre-ON and FLP-OFF dependent manner, under the control of hSyn promoter

   Depositor

   Oscar Marin

   Insert

   hM3D(Gq)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   human Synapsin 1

   Available Since

   May 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-frt-DIO-hM4D(Gi)-mCherry

   Plasmid

   #217398

   Purpose

   Expresses the hM4D(Gi) inhibitory DREADD fused to mCherry, in a Cre-ON and FLP-OFF dependent manner, under the control of hSyn promoter

   Depositor

   Oscar Marin

   Insert

   hM4D(Gi)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   human Synapsin 1

   Available Since

   May 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. eSpCas9(1.1)_NoFlag_ATP1A1_G3_SLBP_G1_Dual_sgRNA

   Plasmid

   #191528

   Purpose

   Vector for tandem expression of SLBP 3'UTR G1 sgRNA in combination with ATP1A1 G3 sgRNA from two independent U6 promoters to facilitate SLBP endogenous tagging by marker free coselection using ouabain

   Depositor

   Yannick Doyon

   Insert

   SLBP 3'UTR G1 sgRNA + ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR; Co-selection via hdr using ouabain

   Expression

   Mammalian

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDest_U6_A2_CG2_T1snp_gRNA

   Plasmid

   #213789

   Purpose

   pDEL114; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type I (SNP) sgRNA

   Depositor

   William Talbot

   Insert

   nrg1 type I SNP sgRNA (nrg1 Zebrafish)
   

   Use

   Tol2 destination vector

   Available Since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cas9-T2A-H2B mCitirine

   Plasmid

   #210471

   Purpose

   Coexpression of Cas9 and H2B mCitrine

   Depositor

   Amro Hamdoun

   Insert

   Cas9-T2A-H2B mCitirine
   

   Use

   CRISPR and Synthetic Biology

   Tags

   mCitrine on C terminal of H2B

   Expression

   Mammalian

   Mutation

   Cas9 contains 3xFLAG tag and nuclear localization…

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAttB-Tub-HA-Wnk

    Plasmid

    #203968

    Purpose

    Fly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expression

    Depositor

    Andreas Jenny

    Insert

    Wnk (most similar to RO variant) (Wnk Fly)
    

    Tags

    HA

    Expression

    Insect

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAttB-Tub-HA-WnkDeltaNT

    Plasmid

    #203969

    Purpose

    Fly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expression

    Depositor

    Andreas Jenny

    Insert

    Wnk (Wnk Fly)
    

    Tags

    HA

    Expression

    Insect

    Mutation

    Nterm deletion

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAttB-Tub-HA-WnkDeltaAI

    Plasmid

    #203970

    Purpose

    Fly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expression

    Depositor

    Andreas Jenny

    Insert

    Wnk (Wnk Fly)
    

    Tags

    HA

    Expression

    Insect

    Mutation

    deletion of autoinhibitory domain

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAttB-Tub-HA-WnkDeltaMID

    Plasmid

    #203971

    Purpose

    Fly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expression

    Depositor

    Andreas Jenny

    Insert

    Wnk (Wnk Fly)
    

    Tags

    HA

    Expression

    Insect

    Mutation

    deletion of middle part

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAttB-Tub-HA-WnkDeltaCC

    Plasmid

    #203972

    Purpose

    Fly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expression

    Depositor

    Andreas Jenny

    Insert

    Wnk (Wnk Fly)
    

    Tags

    HA

    Expression

    Insect

    Mutation

    deletion of coiled coil domains 1 and 2

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAttB-Tub-HA-WnkDeltaCC1

    Plasmid

    #203973

    Purpose

    Fly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expression

    Depositor

    Andreas Jenny

    Insert

    Wnk (Wnk Fly)
    

    Tags

    HA

    Expression

    Insect

    Mutation

    deletion of coiled coil domain 1

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAttB-Tub-HA-WnkDeltaCC2

    Plasmid

    #203974

    Purpose

    Fly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expression

    Depositor

    Andreas Jenny

    Insert

    Wnk (Wnk Fly)
    

    Tags

    HA

    Expression

    Insect

    Mutation

    deletion of coiled coil domain 2

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAttB-Tub-HA-WnkDeltaCT

    Plasmid

    #203975

    Purpose

    Fly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expression

    Depositor

    Andreas Jenny

    Insert

    Wnk (Wnk Fly)
    

    Tags

    HA

    Expression

    Insect

    Mutation

    C-terminal deletion after RFxV motif

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. 3xFlag-CEP83

    Plasmid

    #200442

    Purpose

    Expresses 3xFlag-tagged mouse CEP83 in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Insert

    CEP83 (CCDC41 Human)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSBtet-Bla/mCardinal-TurboID-GAI

    Plasmid

    #198218

    Purpose

    Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with mCardinal, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling

    Depositor

    Eric Davis

    Insert

    mCardinal
    

    Use

    Transposon

    Tags

    3’ part of TurboID, starting with G257 and amino …

    Expression

    Mammalian

    Mutation

    None

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGB-05-08-Vps15

    Plasmid

    #187990

    Purpose

    Plasmid for the protein expression of Vps15 in a bacterial and insect expression system. Internal_Reference: SMC1324

    Depositor

    Sascha Martens

    Insert

    Vps15 (PIK3R4 Human)
    

    Expression

    Bacterial and Insect

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. Ef1a-DIO-Aion-citrine

    Plasmid

    #192571

    Purpose

    AAV-vector for optogenetic long-term silencing of Cre-positive neurons

    Depositor

    Simon Wiegert

    Insert

    Aion
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

    Promoter

    Ef1-alpha

    Available Since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)

    Plasmid

    #166422

    Purpose

    Express pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)

    Depositor

    Kalle Gehring

    Insert

    Plasmodium falciparum PRL
    

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lac1

    Available Since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCW57.1-RFP_mmu-miR-292b-3p_sponge

    Plasmid

    #132623

    Purpose

    Expresses a TurboRFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA sponges

    Depositor

    Constance Ciaudo

    Insert

    stop codon, mmu-miR-292b-3p sponge sequence and polyA signal
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Grp

    Plasmid

    #184292

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Grp

    Depositor

    Bernardo Sabatini

    Insert

    sgRNA-Grp
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Oprl1

    Plasmid

    #184291

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Oprl1

    Depositor

    Bernardo Sabatini

    Insert

    sgRNA-Oprl1
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Rxfp3

    Plasmid

    #184290

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Rxfp3

    Depositor

    Bernardo Sabatini

    Insert

    sgRNA-Rxfp3
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adrb1

    Plasmid

    #184295

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adrb1

    Depositor

    Bernardo Sabatini

    Insert

    sgRNA-Adrb1
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. Akaluc VLP

    Plasmid

    #182212

    Purpose

    YIp for expressing MPyV deltaVP1 (GAL1 promoter) and Akaluc tagged with VP2C (GAL10 promoter).

    Depositor

    Claudia Vickers

    Inserts

    VP2C-Akaluc
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology; Tem imaging

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MDKA-c009

    Plasmid

    #175463

    Purpose

    Protein expression/secretion in mammalian cells. Entire CDS with ectopic signal peptide, V21-D143

    Depositor

    Opher Gileadi

    Insert

    MDK (MDK Human)
    

    Tags

    His6 and PTPRS (Gg) SP

    Expression

    Mammalian

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EZRA-c004

    Plasmid

    #175461

    Purpose

    Protein expression in bacterial cells. FERM domain, M1-Q322.

    Depositor

    Opher Gileadi

    Insert

    EZR (EZR Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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