We narrowed to 6,717 results for: SIM;

1. mCherry-p2A-CreERT2-FRT-kan-FRT

   Plasmid

   #108454

   Purpose

   This plasmid can be used to recombine the mCherry-p2a-CreERT2-pA cassette (or only CreERT2-pA) into a BAC. Also to produce mCherry-p2A-CreERT2 mRNA using SP6.

   Depositor

   Nadia Mercader Huber

   Article

   Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

   Insert

   mCherry-p2a-CreERT2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. L2_lacZgRNA-Cas9-CsA

   Plasmid

   #136138

   Purpose

   Plasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCSN068

   Plasmid

   #101749

   Purpose

   Single copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.

   Depositor

   Rene Verwaal

   Article

   Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

   Inserts

   Cpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
   KanMX marker expression cassette.
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   Heterologous TEF1 promoter from A. gossypii. and …

   Available since

   Jan. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV EF1a DIO iChloC 2A dsRed

   Plasmid

   #70762

   Purpose

   An improved chloride-conducting channelrhodopsin for light-induced inhibition of neuronal activity in vivo. Cre inducible expression (double floxed inversed ORF)

   Depositor

   Thomas Oertner

   Article

   Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

   Inserts

   Channelrhodopsin-2
   red fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   E83Q,E90R,E101S,D156N,T159C

   Promoter

   EF1a

   Available since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-CAG-ArchT-tdTomato

   Plasmid

   #29778

   Purpose

   AAV-mediated expression of ArchT-tdTomato for neuronal inhibition

   Depositor

   Edward Boyden

   Article

   Han et al Front Syst Neurosci. 2011;5:18. Epub 201

   Insert

   ArchT-tdTomato
   

   Use

   AAV; Adeno-associated virus

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available since

   July 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FLAG-hIes2

   Plasmid

   #15355

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

   Insert

   PAP-1 binding protein (INO80B Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pGAPTrap TALEN 2

   Plasmid

   #83369

   Purpose

   Partner to pGAPTrap-TALEN1, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors

   Depositor

   Ed Stanley

   Article

   Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

   Insert

   GAPDH_TALEN2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Oct. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pGAPTrap-TALEN1

   Plasmid

   #83368

   Purpose

   Partner to pGAPTrap-TALEN2, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors

   Depositor

   Ed Stanley

   Article

   Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

   Insert

   GAPDH_TALEN1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Oct. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-CamKII-ArchT-GFP (PV2527)

   Plasmid

   #99039

   Purpose

   AAV-mediated expression of ArchT under the CamKII promoter

   Depositor

   Edward Boyden

   Article

   Han et al Front Syst Neurosci. 2011;5:18. Epub 201

   Has Service

   AAV Retrograde, AAV1, and AAV5

   Insert

   ArchT-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. R702-X31-566: His6-MBP-tev-G-Hs.PDE6D(2-150)

    Plasmid

    #159689

    Purpose

    E. coli protein expression of His6-MBP-tev-G-Hs.PDE6D(2-150)

    Depositor

    Dominic Esposito

    Article

    Dharmaiah et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    His6-MBP-tev-G-Hs.PDE6D(2-150) (PDE6D Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-TRE3G-BE3RA-PGK-Puro

    Plasmid

    #110846

    Purpose

    Lentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    TRE3G

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. plenti6.3/hCx43-stop

    Plasmid

    #27383

    Depositor

    Robin Shaw

    Article

    Smyth et al J Clin Invest. 2010 Jan 4. 120(1):266-79

    Insert

    Gja1 (GJA1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pAX198

    Plasmid

    #173042

    Purpose

    Custom vector for sgRNA library construction

    Depositor

    Brittany Adamson

    Article

    Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

    Insert

    HBB Gene - Second and third exons of ENST00000647020.1 (no intron) and part of the 3' UTR (HBB Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMT1-24X-NOG(CmYLCV)

    Plasmid

    #202014

    Purpose

    T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the CmYLCV promoter.

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Inserts

    dCas9-24XGP41
    NbGP41P-sfGFP-VP64-GB1
    

    Use

    Tags

    GB1 and sfGFP

    Expression

    Plant

    Mutation

    Promoter

    CmYLCV

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21b-Caspase-3(D3A: D175A D28A D9A)

    Plasmid

    #90090

    Purpose

    Expresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cells

    Depositor

    Clay Clark

    Article

    Bose et al Biochemistry. 2003 Oct 28;42(42):12298-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D175A D28A D9A

    Promoter

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-SynI-FlpOn-CreOff-MCS-P2A-mCherry

    Plasmid

    #176277

    Purpose

    Viral vector for co-expression of a CDS of interest and mCherry in cells expressing Flp AND NOT Cre driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    MCS-P2A-mCherry
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin I

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CamKII-ArchT-GFP

    Plasmid

    #37807

    Purpose

    Viral expression of ArchT-GFP for optogenetic inhibition

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Insert

    ArchT-GFP
    

    Use

    AAV; Adeno-associated virus

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETM33_WDR5_WD

    Plasmid

    #178491

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    WDR5_WD (WDR5 Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GW1-mito-roGFP1-mApple

    Plasmid

    #107218

    Purpose

    Mammalian expression plasmid to target the roGFP1-mApple sensor to the mitochondrial matrix.

    Depositor

    Mathew Tantama

    Article

    Norcross et al ACS Sens. 2017 Nov 22;2(11):1721-1729. d

    Insert

    roGFP1-mApple
    

    Use

    Tags

    4xCox8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-BE3-FNLS(RA)

    Plasmid

    #112671

    Purpose

    CMV expression vector for BE3-FNLS construct (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3-FNLS
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    NLS sequence at the N-terminus and D10A

    Promoter

    CMV

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge