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pBWB162

Plasmid

#140634

Purpose

pBAV1k-lacI-Trc-mCherry base, lacI repression vector for ADP1

Depositor

Keith Tyo

Article

Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-Tornado-split-nLuc

Plasmid

#212612

Purpose

For the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

Tornado-CVB3-split-nLuc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

C373T mutation in CVB3

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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EF06R

Plasmid

#42940

Depositor

Eline Luning Prak

Article

Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

Insert

Human L1 element (L1RE1 Human)

Use

Tags

EGFP (opposite strand)

Expression

Mammalian

Mutation

Promoter

CMV MIE, L1 5'UTR

Available since

April 25, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-GR11

Plasmid

#89105

Purpose

mammalian expression of full-length human glucocorticoid receptor in pCMV5

Depositor

Elizabeth Wilson

Article

Kupfer et al J Biol Chem. 1993 Aug 15;268(23):17519-2

Insert

human glucocorticoid receptor (NR3C1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Broccoli-aptNFkB#5

Plasmid

#124362

Purpose

Overexpression of bifunctional circular RNA (Broccoli fluorescence and NFkB binding)

Depositor

Samie Jaffrey

Article

Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

Insert

Tornado-F30-Broccoli-aptNFkB#5

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6+27

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pRNA2-(A)128

Plasmid

#174006

Purpose

Used to generate stable mRNA for heterologous expression experiments (includes polyA tail)

Depositor

Stephen Ikeda

Article

Williams et al Front Neurosci. 2010 Nov 4;4:181. doi: 1

Insert

eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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miniCMV-(mNeonGreen)4-tDeg

Plasmid

#129402

Purpose

(mNeonGreen)4-tDeg fluorogenic protein

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

(mNeonGreen)-tDeg

Use

Tags

Expression

Mammalian

Mutation

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pKJ0377

Plasmid

#228712

Purpose

Mammalian expression of human codon-optimized enhanced Bxb1 (epBxb1 mutant T166R)

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Science 0, eadr6006

Insert

Bxb1_T166R

Use

Tags

Expression

Mammalian

Mutation

T166R

Promoter

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-mutTornado-split-nLuc

Plasmid

#212707

Purpose

Tornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

mutTornado-CVB3-split-nLuc

Use

Tags

Expression

Mutation

Deleted the partial 3'ribozyme sequence in T…

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ0463

Plasmid

#228715

Purpose

Bacterial expression of epT7 RNAP; for protein production and purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Science 0, eadr6006

Insert

Sumo_epT7

Use

Tags

Expression

Bacterial

Mutation

T3M/G47A/E643G

Promoter

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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