Addgene

We narrowed to 17,298 results for: Eomes

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pAav-MCS-PQS1-3xFLAG

Plasmid

#84883

Purpose

rAAV-based template for genome engineering of protein C-termini containing PQS1 and 3xFLAG tags and a selection cassette

Depositor

Sven Eyckerman

Article

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Insert

Multicloning sites and selection cassette

Use

AAV

Tags

Expression

Mutation

Promoter

PGK

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pBT259_(pRosa26-G-tTA2)

Plasmid

#36878

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron
Neo
diphteria toxin A

Use

Tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS2236

Plasmid

#111889

Purpose

High copy number plasmid containing Ple321 (TUBB3 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple321 (TUBB3 Human)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Ple321 (TUBB3 MiniPromoter)

Available since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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PX4

Plasmid

#223340

Purpose

Mapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

ABCD1-EGFP-miniTurboID-3HA (PXA1 Mustard Weed)

Use

Tags

EGFP-miniTurboID-3HA

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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PX3

Plasmid

#223339

Purpose

Mapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

ABCD1-miniTurboID-EGFP-3HA (PXA1 Mustard Weed)

Use

Tags

miniTurboID-EGFP-3HA

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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PX2

Plasmid

#223338

Purpose

Mapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

ABCD1-EGFP-TurboID-3HA (PXA1 Mustard Weed)

Use

Tags

EGFP-TurboID-3HA

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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PX1

Plasmid

#223337

Purpose

Mapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

ABCD1-TurboID-EGFP-3HA (PXA1 Mustard Weed)

Use

Tags

TurboID-EGFP-3HA

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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MT2

Plasmid

#223330

Purpose

Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

3HA-TurboID-EGFP-OMP25 (Synj2bp Rat)

Use

Tags

3HA-TurboID-EGFP

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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MT3

Plasmid

#223331

Purpose

Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

3HA-EGFP-miniTurboID-OMP25 (Synj2bp Rat)

Use

Tags

3HA-EGFP-miniTurboID

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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MT4

Plasmid

#223332

Purpose

Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

3HA-miniTurboID-EGFP-OMP25 (Synj2bp Rat)

Use

Tags

3HA-miniTurboID-EGFP

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-IDH2-WT

Plasmid

#87926

Purpose

Expresses IDH2 (wild-type) in mammalian cells

Depositor

Reproducibility Project Cancer Biology

Article

Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

Insert

isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-FRT-stop-FRT(FSF)- loxP-stop-loxP(LSL)-SP-HA-HRPtm-WPRE-bGHpolyA

Plasmid

#197044

Purpose

In situ cell-surface proteome extraction by extracellular labeling (iPEEL)

Depositor

Liqun Luo

Article

Shuster et al Neuron. 2022 Dec 7;110(23):3882-3896.e9.

Inserts

pCAG-FSF-LSL
SP-HA-HRPtm
WPRE-bGHpolyA

Use

Tags

HA, myc

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBT272_(pRosa26-GTET)

Plasmid

#36882

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator
diphteria toxin A

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS2242

Plasmid

#111897

Purpose

ssAAV genome with Ple321 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple321 (TUBB3 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Ple321 (TUBB3 MiniPromoter)

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF6-Lck-TurboID

Plasmid

#159433

Purpose

expresses Lck-TurboID in mammalian cells

Depositor

Arthur Salomon

Article

Chua et al J Proteome Res. 2020 Nov 13. doi: 10.102

Inserts

Lck (LCK Human)
TurboID (BirA mutant)

Use

Tags

HA

Expression

Mammalian

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

EF-1a

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEMS2274

Plasmid

#111900

Purpose

ssAAV genome with Ple344 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple344 (TUBB3 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Ple344 (TUBB3 MiniPromoter)

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS2273

Plasmid

#111899

Purpose

ssAAV genome with Ple343 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple343 (TUBB3 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Ple343 (TUBB3 MiniPromoter)

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBT270_(pCA-G-intron(Neo)-tTA2-iiTRE-tdT3Mycii)

Plasmid

#36881

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS2272

Plasmid

#111898

Purpose

ssAAV genome with Ple342 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple342 (TUBB3 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Ple342 (TUBB3 MiniPromoter)

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-IDH2-R172K

Plasmid

#87927

Purpose

Expresses IDH2 (R172K mutant) in mammalian cells

Depositor

Reproducibility Project Cancer Biology

Article

Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

Insert

isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)

Use

Tags

none

Expression

Mammalian

Mutation

R172K

Promoter

CMV

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge