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Plasmid#227636Purposec-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.DepositorInsertktrA-yfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUdOm2.0
Plasmid#210300PurposeEmpty backbone with ampicillin resistance for high expression in mammalian cells with an added 5'UTRDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMpGWBhis03-Citrine-NLS
Plasmid#188558PurposeBinary vector for the expression of Citrine-NLS with MpIGPDm selective marker in Marchantia polymorpha (igpd mutants) .DepositorInsertCitrine-NLS and MpIGPDm
UseCRISPR; Entry vectorTagsExpressionMutationPromoterAvailable sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pR
Plasmid#193746PurposeExpresses LuxR under the control of BBa_J23102 constitutive promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertLuxR
UseTagsExpressionBacterialMutationPromoterBBa_J23102Available sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTA2EGFP
Plasmid#193756PurposeExpresses EGFP under the control of PLTetO-1 promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertEGFP
UseTagsExpressionBacterialMutationPromoterPLTetO-1Available sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJA2LUXR(F)
Plasmid#193755PurposeExpresses LuxR in forward direction under the control of BBa_J23102 constitutive promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertLuxR
UseTagsExpressionBacterialMutationPromoterBBa_J23102Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcloDF-amp
Plasmid#184844PurposeBackbone that confers Ampicillin resistance, contains cloDF oriDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a_Msm_RbpA
Plasmid#87928PurposeMycobacterium smegmatis RpbA in pET21a (ampR) used to co-express with Msm SigA for crystallization purposesDepositorInsertRbpA
UseTagsExpressionBacterialMutationPromoterT7PAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
36INS-pTet_GB
Plasmid#78681PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [G:36INS-pTet:B]DepositorInsertInsulated pTet Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
ARK2a
Plasmid#81017PurposeE.coli BW25113 + JBEI-9310 (pA5c-MevT(O)-T21-MKco, JBx_039538) + JBEI-9314(pTrc99a-PMDsc, JBx_039366)DepositorInsertsMevTo-BBa1002-pTrc-Mkco
PMD
UseTagsExpressionMutationPromoterLacUV5 and Trc promoterAvailable sinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS-pTet_FB
Plasmid#78680PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [F:36INS-pTet:B]DepositorInsertInsulated pTet Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS-pTet_EB
Plasmid#78679PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [E:36INS-pTet:B]DepositorInsertInsulated pTet Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS-pTet_AB
Plasmid#78678PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [A:36INS-pTet:B]DepositorInsertInsulated pTet Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS1-pBAD_GB
Plasmid#78673PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [G:36INS1-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS1-pBAD_FB
Plasmid#78672PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [F:36INS1-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS1-pBAD_EB
Plasmid#78671PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [E:36INS1-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
p11-LacY-wtx1
Plasmid#69056PurposeThe reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.DepositorUseTagsA177CExpressionMutationPromoterpBAD and pLacAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPANI2A2E2-Crimson
Plasmid#193758PurposeExpresses E2-Crimson under the control of PANI2 synthetic promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertE2-Crimson
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPANI2 synthetic promoter carrying binding sites f…Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAB1
Plasmid#62547Purposebroad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.DepositorInserteGFP
UseBroad host range plasmidTagsExpressionBacterialMutationPromoterpTrcAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP Zeo (637-7)
Plasmid#17449Purpose3rd gen lentiviral eGFP expression vector, CMV promoter, ZeoDepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only