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pCDNA4/TO-GFP-SPOP (R354H)

Plasmid

#197010

Purpose

Mammalian inducible expression vector for GFP-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

R354H

Promoter

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-GFP-SPOP (R221C)

Plasmid

#197009

Purpose

Mammalian inducible expression vector for GFP-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

R221C

Promoter

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV R354H SPOP 28-359

Plasmid

#197011

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Human)

Use

Tags

6His-SUMO-TEV

Expression

Bacterial

Mutation

R354H

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-SPOP-myc (R121Q)

Plasmid

#197007

Purpose

Mammalian inducible expression vector for myc-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

R121Q

Promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-SPOP-myc (R354H)

Plasmid

#197006

Purpose

Mammalian inducible expression vector for myc-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

R354H

Promoter

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-SPOP-myc (R221C)

Plasmid

#197005

Purpose

Mammalian inducible expression vector for myc-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

R221C

Promoter

Available since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-SPOP-myc (BACK)

Plasmid

#197021

Purpose

Mammalian inducible expression vector for myc-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Y353E

Promoter

Available since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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nAChR beta2 WT

Plasmid

#24272

Depositor

Henry Lester

Article

Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

Insert

nAChR beta2 WT (Chrnb2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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nAChR alpha4 WT

Plasmid

#24271

Depositor

Henry Lester

Article

Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

Insert

nAChR alpha4 WT (Chrna4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only
pMZ376

Plasmid

#74213

Purpose

Combined sgRNA/Cas9 vector with KanMX marker

Depositor

Mikel Zaratiegui

Inserts

Cas9
rrk1:sgRNA

Use

CRISPR

Tags

Expression

Yeast

Mutation

Silent muation of the CspCI site

Promoter

adh1 and rrk1

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pMCh-1468

Plasmid

#82418

Purpose

Plasmid for expression of HA-tagged Nematostella vectensis GW182 in mammalian cells

Depositor

Marina Chekulaeva

Article

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

nvGW182

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMCh-1469

Plasmid

#82419

Purpose

Plasmid for expression of NHA-tagged Nematostella vectensis GW182 in mammalian cells

Depositor

Marina Chekulaeva

Article

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

nvGW182

Use

Tags

NHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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Twitch-1 pcDNA3

Plasmid

#49812

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-1

Use

Tags

ECFP and cpCit174

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only
Twitch-4 pcDNA3

Plasmid

#49533

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-4

Use

Tags

ECFP and cpCit174

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only
pMCh-1016

Plasmid

#92255

Purpose

Plasmid for expression of HA-tagged human TNRC6C CED in mammalian cells

Depositor

Witold Filipowicz, Marina Chekulaeva

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

TNRC6C CED (TNRC6C Human)

Use

Tags

HA

Expression

Mammalian

Mutation

.C-terminal effector domain (CED): aa 1369-1690

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMCh-133

Plasmid

#92256

Purpose

Plasmid for expression of NHA-tagged human TNRC6C CED in mammalian cells

Depositor

Witold Filipowicz, Marina Chekulaeva

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

TNRC6C CED (TNRC6C Human)

Use

Tags

NHA

Expression

Mammalian

Mutation

.C-terminal effector domain (CED): aa 1369-1690

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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Twitch-2B pcDNA3

Plasmid

#49531

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2B

Use

Tags

ECFP and cpCit174

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
Twitch-3 pcDNA3

Plasmid

#49532

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-3

Use

Tags

ECFP and cpCit174

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMCh-658

Plasmid

#92257

Purpose

Plasmid for expression of NHA-tagged human TNRC6C CED in mammalian cells with mutated PAM2 motif

Depositor

Witold Filipowicz, Marina Chekulaeva

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

TNRC6C CED (TNRC6C Human)

Use

Tags

NHA

Expression

Mammalian

Mutation

C-terminal effector domain (CED): aa 1369-1690 wi…

Promoter

CMV

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMCh-1474

Plasmid

#82420

Purpose

Plasmid for expression of HA-tagged N-terminal region of Nematostella vectensis GW182 (aa 1-1158) in mammalian cells

Depositor

Marina Chekulaeva

Article

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

nvGW182 NED

Use

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 1159-1698 from nvGW182

Promoter

CMV

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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