We narrowed to 589 results for: pcDNA3 expression vector

1. pTS2307-Tier1-2xcHS4-PPGK-NLuc_TB

   Plasmid

   #169550

   Purpose

   Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a tandem repeat of a 5' cHS4 insulator sequence (2xcHS4-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   cHS4-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTS2304-Tier1-Tactb-PPGK-NLuc_TB

   Plasmid

   #169549

   Purpose

   Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   Tactb-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTS2387-Tier1-PhCMV-p2a_mTagBFP2

   Plasmid

   #169524

   Purpose

   Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven P2A-fused monomeric Tag blue fluorescent protein 2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTS2396-Tier1-PhCMV-4xCOX8_Ypet

   Plasmid

   #169534

   Purpose

   Tier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet localized to the mitochondria (PhCMV-4xCOX8-YPet-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven miochondria-localized yellow fluorescent protein Ypet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTS2410-Tier1-PhCMV-Myr_mTagBFP2

   Plasmid

   #169535

   Purpose

   Tier-1 vector encoding PhCMV-controlled plasma membrane-tethered mTagBFP2 (PhCMV-myr-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven membrane-localized monomeric Tag blue fluorescent protein 2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA

   Plasmid

   #194725

   Purpose

   based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFB

   Depositor

   Rudolf Jaenisch

   Article

   Haiting et al bioRxiv

   Insert

   AsCpf1 (MAFB, AAVS1 Acidaminococcus sp.)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D908A

   Promoter

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTS2388-Tier1-PhCMV-p2a_Ypet

   Plasmid

   #169525

   Purpose

   Tier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet fused to a "self-cleaving" peptide p2a (PhCMV-p2a_YPet-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven P2A-fused yellow fluorescent protein Ypet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTS2389-Tier1-PhCMV-p2a_mRuby

   Plasmid

   #169526

   Purpose

   Tier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mRuby2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven P2A-fused enhanced yellow fluorescent protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTS1024-Tier1-PRPBSA-YPet-p2A-PuroR

   Plasmid

   #169499

   Purpose

   Tier-1 vector encoding PRPBSA-driven YPet in combination with a marker for puromycine selection (PRPBSA-YPet-p2A-PuroR-pA)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven YPet-P2aA-PuroR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PRBSA

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTS1047-Tier1-PhCMV-YPet-p2A-PuroR

    Plasmid

    #169498

    Purpose

    Tier-1 vector encoding PhCMV-driven YPet in combination with a marker for puromycin selection (PhCMV-YPet-p2A-PuroR-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven YPet-P2aA-PuroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTS2308-Tier1-pGL3-PPGK-NLuc_TB

    Plasmid

    #169551

    Purpose

    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    pGL3-insulator-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pVH333-1-Tier1-PhCMV-dCas9-3xNLS-VP64

    Plasmid

    #169597

    Purpose

    Tier-1 vector encoding PhCMV-driven dCas9-3xNLS-VP64 expression (PhCMV-dCas9-3xNLS-VP64-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    dead S.pyogenes Cas9 - VP64 fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pVH19-Tier1-PhCMV-p300core

    Plasmid

    #169572

    Purpose

    Tier-1 vector encoding PhCMV-driven histone acetyltransferase p300 core domain expression (PhCMV-p300core-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven P300core (EP300 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDEST-ORF-V2

    Plasmid

    #73638

    Purpose

    Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 2 (V2).

    Depositor

    Darren Saunders

    Article

    Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

    Type

    Empty backbone

    Use

    Tags

    Venus fragment 2 (V2)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDEST-ORF-V1

    Plasmid

    #73637

    Purpose

    Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 1 (V1)

    Depositor

    Darren Saunders

    Article

    Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

    Type

    Empty backbone

    Use

    Tags

    Venus fragment 1 (V1)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHM024Env

    Plasmid

    #137691

    Purpose

    Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

    Depositor

    Anjali Joshi

    Article

    Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

    Insert

    HIV-1 Envelope Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHM123DEnv

    Plasmid

    #137668

    Purpose

    Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

    Depositor

    Anjali Joshi

    Article

    Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

    Insert

    HIV-1 Envelope Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHM130Env

    Plasmid

    #137665

    Purpose

    Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

    Depositor

    Anjali Joshi

    Article

    Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

    Insert

    HIV-1 Envelope Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHM033Env

    Plasmid

    #137672

    Purpose

    Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

    Depositor

    Anjali Joshi

    Article

    Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

    Insert

    HIV-1 Envelope Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHM019Env

    Plasmid

    #137685

    Purpose

    Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

    Depositor

    Anjali Joshi

    Article

    Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

    Insert

    HIV-1 Envelope Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge