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pGP-AAV-CAG-iGABASnFR2n-WPRE

Plasmid

#218884

Purpose

AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Insert

iGABASnFR2n

Use

AAV

Tags

Expression

Mammalian

Mutation

S99A F104H R168P

Promoter

CAG

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-CAG-iGABASnFR2-WPRE

Plasmid

#218883

Purpose

AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Insert

iGABASnFR2

Use

AAV

Tags

Expression

Mammalian

Mutation

S99A F102Y F104Y L178S

Promoter

CAG

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.0-Pct5.1-crRNA(AarI)

Plasmid

#191633

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer), entry plasmid for crRNA cloning

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pVRa20_992

Plasmid

#49673

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF20_992

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.9RT2-Pct5.1-crRNA(cadR)-RT(ΔcadR)

Plasmid

#191647

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cadR-targeting spacer) cadR-deleting repair template,used for cadR deletion

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-GFAP-iGABASnFR2n-WPRE

Plasmid

#218872

Purpose

AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Insert

iGABASnFR2n

Use

AAV

Tags

Expression

Mammalian

Mutation

S99A F104H R168P

Promoter

GFAP

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10RFP-Pct5.1-crRNA(AarI)-RFP

Plasmid

#192273

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(AarI)-RFP, entry plasmid for crRNA cloning

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.1RT1-Pct5.1-crRNA(dadR)-RT(ΔdadR)

Plasmid

#191635

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer) dadR-deleting repair template, used for dadR deletion

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMA3379

Plasmid

#46874

Purpose

A lentiviral vector for the doxycycline-inducible expression of soluble guanylate cyclase1 alpha3

Depositor

Troy Stevens, Mikhail Alexeyev

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

soluble guanylate cyclase 1alpha3 (Gucy1a3 Rat)

Use

Lentiviral

Tags

Myc

Expression

Mutation

Promoter

TRE-Tight

Available since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pEJS481-pCDest2-AcrE2-mTagBFP2-IRES

Plasmid

#85749

Purpose

Expresses anti-CRISPR protein from Type I-E and mTagBFP2

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Inserts

Type I-E anti-CRISPR
mTagBFP2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pMA3431

Plasmid

#46876

Purpose

A lentiviral vector for the doxycycline-inducible expression of soluble guanylate cyclase1 alpha3 mutant R592Q

Depositor

Troy Stevens, Mikhail Alexeyev

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

soluble guanylate cyclase 1alpha 3 (Gucy1a3 Rat)

Use

Lentiviral

Tags

Myc

Expression

Mutation

R592Q

Promoter

TRE-Tight

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pVRa11_3726

Plasmid

#49662

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF11_3726

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pPIP1;4-NLS-3xmCitrine

Plasmid

#192524

Purpose

Fluorescent reporter for plant responses to bacterial infection

Depositor

Gitta Coaker

Article

Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

Insert

PLASMA MEMBRANE INTRINSIC PROTEIN 1;4 (PIP1;4 Mustard Weed)

Use

Tags

3xmCitrine and NLS

Expression

Plant

Mutation

Promoter

PIP1;4

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pS238D1::tse5-CT

Plasmid

#192958

Purpose

For expression of Tse5-CT (1169-1317) in P. putida for biological function studies

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

tse5-CT

Use

Tags

Expression

Bacterial

Mutation

encodes for tse5 (1169-1317)

Promoter

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.6RT4-Pct5.1-crRNA(hcdR)-RT(ΔhcdR)

Plasmid

#191646

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(hcdR-targeting spacer) hcdR-deleting repair template, used for hcdR deletion

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pPodA11

Plasmid

#162843

Purpose

Expression protein designed PodA10 from a TEV cleavable IPTG inducible plasmid with ampicillin resistance

Depositor

Dianne Newman

Article

VanDrisse et al Proc Natl Acad Sci U S A. 2021 Mar 23;11

Insert

Protein Designed Pyocyanin demethylase

Use

Tags

TEV-cleavable 6x-His tag

Expression

Bacterial

Mutation

A53N, I73T, A87V, M99V, A129T from WT PodA

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pVRa32_3724

Plasmid

#49690

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF32_3724

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRa26_837

Plasmid

#49679

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF26_837

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA3411

Plasmid

#46877

Purpose

A retroviral vector for the constitutive expression of soluble guanylate cyclase1 beta3 double mutant (E473K and C541D)

Depositor

Troy Stevens, Mikhail Alexeyev

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

soluble guanylate cyclase1 beta 3 (Gucy1b3 Rat)

Use

Retroviral

Tags

Expression

Mutation

E473K, C541D

Promoter

LTR

Available since

Aug. 14, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pMF440

Plasmid

#62550

Purpose

Broad host range plasmid for constitutive expression of mCherry. Designed for expression in Pseudomonas aeruginosa.

Depositor

Michael Franklin

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

trc

Available since

April 1, 2015

Availability

Academic Institutions and Nonprofits only

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